Introduction
Real-time PCR, also known as quantitative PCR (qPCR), is a molecular biology technique that amplifies and simultaneously quantifies specific DNA sequences in real time, using fluorescent reporters to monitor the accumulation of amplified DNA during each cycle of the polymerase chain reaction, and it is widely used in research, clinical diagnostics, and biotechnology. In operation, the reaction mixture contains the DNA template, primers, a DNA polymerase, and a fluorescent probe or dye, and the thermal cycler amplifies the target sequence; after each cycle, the instrument measures the fluorescence, which increases in proportion to the amount of amplified DNA, and the cycle number at which the fluorescence exceeds a threshold, called the cycle threshold (Ct) value, is related to the initial amount of the target DNA, allowing quantitative measurement, and the fluorescence can be generated by intercalating dyes, such as SYBR Green, which bind to double-stranded DNA, or by sequence-specific probes, such as TaqMan probes, which provide higher specificity. Real-time PCR systems are used for gene expression analysis, for quantifying mRNA and microRNA, for pathogen detection and viral load measurement in clinical diagnostics, for genotyping, for copy number analysis, for detecting genetically modified organisms, for food safety testing, for environmental monitoring, and for many research applications, and they offer high sensitivity, wide dynamic range, and the ability to analyze multiple targets in a single run using multiplexing. With their quantitative capability, real-time PCR is an essential technique in molecular biology and diagnostics.
Real Time PCR LRTP-T2011
Real-Time with a reaction capacity ranging from 10 to 50 µl, accommodates diverse sample volumes. It features high-speed thermal cycling, enabling rapid amplification. The system delive...
Real Time PCR LRTP-T2012
Real Time offers a block temperature range from 0℃ to 105℃, providing precise thermal control. It incorporates a non-fiber optical system, ensuring minimal signal loss. The enhanced si...
Real Time PCR LRTP-T2010
Real Time has a capacity of 96 wells × 0.1 ml and efficiently handles high-throughput analysis. It provides high signal sensitivity, ensuring precise data acquisition. The system enable...
Real Time PCR LRTP-T2060
Real Time has a capacity of 48×0.2ml, making it compatible with both 8-tube strips and individual single-tubes. It can accommodate volumes from 20µl to 120µl, allowing flexibility for...
Real Time PCR LRTP-T2043
Real Time can scan 96 samples across 6 fluorescent channels within 5 seconds. It features a strong fluorescence signal with low background noise and high sensitivity. The long-lasting, a...
Real Time PCR LRTP-T2042
Real Time is a fluorescent quantitative system with a throughput of 96 wells and supports up to 6 channels. Offers a gradient temperature control range of 30 to 100°C with a maximum tem...
Real Time PCR LRTP-T2040
Real Time a powerful, precise, modular design thermal cycler system with 4-fluorescence detection channel. Based on advanced Peltier-based technology with high amplification efficiency u...
Real Time PCR LRTP-T2041
Real Time is a superior, high-performance benchtop open system with 5 fluorescent detection channels. It utilizes an . Its 3D-style design ensures even pressure distribution and heat ret...
Real-Time PCR LRTP-T2050
Real-Time is fully innovative in its unique modular and adjustable design for monitoring the amplification of individual molecules throughout the process. Configured with quantitative am...
Real Time PCR LRTP-T2031
Real Time adopts American Marlow customized Peltier module, double channel and double 8-wells blocks design with analysis software that can run two different design at a same time. It is...
Real Time PCR LRTP-T2030
Real Time is a portable unit comes with sample capacity of 16×0.2 ml (2×8 well, double block), offers emission wavelength ranges from F1: 520 to 540 nm and factory calibrated dyes of F...
Standards
ISO 20836:2021-Microbiology of the food chain-PCR for the detection of microorganisms-Thermal performance testing of thermal cyclers
ISO 22174:2024-Microbiology of the food chain-PCR for the detection and quantification of microorganisms-General requirements and definitions
ISO 20395:2019-Biotechnology-Requirements for evaluating the performance of quantification methods for nucleic acid target sequences-qPCR and dPCR
ISO 17822:2020-In vitro diagnostic test systems-Nucleic acid amplification-based examination procedures-Laboratory quality practice guide
Complies with the above standards, but is not limited to them.
Features
1. Real-time PCR (qPCR) system for nucleic acid amplification and quantification.
2. Fluorescence detection during amplification for quantitative and qualitative analysis.
3. Suitable for molecular biology, clinical diagnostics, genomics, forensics and research.
4. Includes thermal cycler, fluorescence detector, software and operation manual.
5. Supports gene expression, pathogen detection, genotyping and absolute quantification.
FAQ
Q1: What testing scenarios is this system mainly applied to?
A: The real-time PCR system is used in molecular biology, clinical diagnostics, genomics research, forensic science, pharmaceutical development, agricultural biotechnology, food safety and environmental monitoring to perform real-time polymerase chain reaction (qPCR) for nucleic acid amplification, quantification and detection. It supports gene expression analysis and quantification, pathogen detection and viral load measurement (COVID, HIV, HBV, etc.), genotyping and SNP analysis, genetically modified organism (GMO) detection, food safety pathogen testing, forensic DNA analysis, environmental microbial detection, and absolute/relative nucleic acid quantification. The real-time fluorescence detection enables quantification without post-PCR processing, providing fast, sensitive results. It is essential for molecular biology, clinical, forensic, agricultural, food and environmental laboratories requiring real-time nucleic acid amplification and quantification for gene expression, pathogen detection, genotyping, viral load measurement, GMO testing, food safety and research, where real-time fluorescence detection provides sensitive, specific, quantitative nucleic acid analysis without post-PCR processing, supporting clinical diagnostics, research, food safety, environmental monitoring and forensic applications requiring rapid, accurate nucleic acid detection and quantification.
Q2: What standards does the system comply with?
A: The real-time PCR system complies with relevant IEC 61010 laboratory electrical safety standards and applicable GB/T standards for molecular biology equipment. Clinical diagnostic applications follow validated laboratory procedures, applicable medical device regulations and quality management standards including ISO 15189 where accredited. The instrument complies with relevant electromagnetic compatibility standards. For diagnostic use, assays should be validated per applicable regulations.
Q3: What is the warranty period for the whole machine and key components?
A: The whole real-time PCR system is covered by a 12-month warranty. The thermal cycler, fluorescence detector, optics, controller and software are covered, while sample tubes, plates, fuses and accessories are consumables or accessories. Damage from improper use, sample spillage or unauthorized repair is excluded. Extended service contracts are available for this critical laboratory instrument.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained molecular biology personnel familiar with qPCR principles, assay design, fluorescence detection and data analysis. We provide comprehensive training, operation manuals and application support. Proper reaction setup, primer/probe design, cycle programming, fluorescence channel selection, contamination prevention and data interpretation are essential for successful qPCR.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Temperature calibration and fluorescence verification recommended every 6-12 months. Sample block condition checked periodically. Optical system alignment and calibration per manufacturer schedule. Routine decontamination recommended, particularly for clinical and forensic use. Software updates installed as available. Full system performance verification annually. Routine contamination monitoring for diagnostic use.
Q7: Are supporting consumables universal?
A: PCR tubes, plates, seals, reagents, enzymes and probes are available from multiple molecular biology suppliers, though tube/plate compatibility with the block should be verified. Long-term consumable cost depends on experiment volume and reagent choices.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes block format (96-well, 384-well), fluorescence channels, detection modes, fast cycling, connectivity and software features. Additional accessories and consumables can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, real-time PCR systems include on-site installation, commissioning, calibration and operator training by certified application specialists. Site preparation guidance is provided before delivery to ensure environmental and space requirements are met.
Q10: What types of samples are compatible with this equipment?
A: The real-time PCR system amplifies and detects nucleic acid samples (DNA, cDNA from RNA) in compatible tubes or plates within the block format and fluorescence specification. Samples should be properly extracted nucleic acids or crude samples with validated protocols. RNA requires reverse transcription before qPCR (RT-qPCR). Different fluorophores require specific fluorescence channels. Users should follow proper qPCR setup including master mix preparation, primer/probe design, template concentration, cycle parameters, fluorescence channel selection, use appropriate tubes/plates for the block, prevent contamination with dedicated pre- and post-PCR areas, include appropriate controls (NTC, standard curve, positive controls), and follow good molecular biology practices. For clinical diagnostic use, assays should be validated and performed per established SOPs in a quality-controlled environment. Data analysis should follow MIQE guidelines for research publications.
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