Introduction
Real Time is a superior, high-performance benchtop open system with 5 fluorescent detection channels. It utilizes an . Its 3D-style design ensures even pressure distribution and heat retention. Fitted with a unique sliding track for convenient sample handling. It Includes advanced programming functions, including gradient and touch-down settings. Designed for low noise, energy efficiency, and longevity. This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.Applications
Our Real Time supports diverse applications like absolute/relative quantification and melting curve analysis. Applicable for Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA and Pathogen Detection.Specifications
| Fluorescence Detection Channel | 5 |
| Reactions Per Run | 96 |
| Block Format | 96-well, 0.2 ml |
| Blocks Compatibility | 96-well plates, 12-well strip tubes, and 8-well strip tubes |
| Color Combinations | UP to 5 |
| Light Source | High-brightness monochrome |
| Detector | Highly sensitive cold light |
| Dynamic Detection Range | 10² to 10¹º |
| Sensitivity | 1 copy |
| Reaction Volume | 15 to 100 µl |
| Excitation Source | White |
| Excitation Filters | Channel 1: 470 nm, Channel 2: 525 nm, Channel 3: 585 nm, Channel 4: 625 nm, Channel 5: 682.5 nm |
| Detection Filters | Channel 1: 520 nm, Channel 2: 570 nm, Channel 3: 620 nm, Channel 4: 675 nm, Channel 5: 725 nm |
| Fluorescent Dyes | Channel 1: / Channel 2: /////CY3 Channel 3: / Channel 4: CY5 Channel 5: CY5.5 |
| Block Material | Peltier |
| Temperature Range | 0°C to 100°C |
| Temperature Accuracy | ± 0.1 °C |
| Temperature Uniformity | ± 0.4 °C (10 sec after reaching 95°C) ± 0.2 °C (10 sec after reaching 55°C) |
| Temperature Control Mode | Block and Tube |
| Maximum Ramp Rate | 6°C/sec |
| Gradient Range | 30°C to 100°C |
| Chemistry | Open system for all available real-time chemistries including Taqman Hydrolysis probes, Green, dual-labelled and MB probes, probes, and Molecular Beacons |
| PC Operation System | Windows XP//Windows7/ Windows 8/ Windows 10 |
| Machine Operation System | Linux |
| A8 | |
| Network | /Wi-Fi |
| Application Available | Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection |
| Power Supply | 220V/50HZ |
| Dimension (W×D×H) | 592 × 440 × 280mm |
| Packaging Dimension (W×D×H) | 840 × 710 × 530mm |
| Weight | 60 Kg |
Features
High-quality Peltier plates for enhanced amplification
Features standard gold-plating to improve heat conduction
Constructed from 6mm thick aluminum alloy for durability
Smooth curves and a fresh color scheme with Adjustable footing
An independent and interactive software analysis module
Built on a Linux-based operating system
Equipped with an A8 for an enhanced control experience
Compatible with various reagents and consumables
FAQs
1. What are the key factors to consider when selecting this analytical equipment for laboratory use?
When selecting this analytical equipment, key factors include: 1) The instrument's precision and accuracy to meet experimental requirements; 2) Compatibility with the types of samples and applications you will be working with; 3) Ease of use and maintenance requirements; 4) Safety features to protect operators and samples; 5) Calibration and certification requirements; 6) Cost-effectiveness and long-term reliability; 7) Technical support and service availability from the supplier. Evaluating these factors ensures that the instrument meets your laboratory's specific needs.
2. How often should this analytical equipment be calibrated to ensure accurate results?
It is recommended to calibrate this analytical equipment at least once every 6-12 months under normal usage conditions. More frequent calibration (every 3-6 months) may be necessary if: 1) The instrument is used continuously for extended periods; 2) It is exposed to harsh environmental conditions (temperature fluctuations, humidity, dust); 3) It is used for critical applications requiring high precision; 4) The instrument has been moved or subjected to physical shock; 5) Calibration checks indicate significant deviations from standard values. Always follow the manufacturer's calibration guidelines.
3. What are the common maintenance procedures required for this analytical equipment?
Common maintenance procedures include: 1) Regular cleaning of the instrument's exterior and sample contact surfaces to prevent contamination and ensure proper operation; 2) Inspection of moving parts (if applicable) for wear and lubrication as recommended by the manufacturer; 3) Replacement of consumable parts (filters, bulbs, reagents) according to usage guidelines; 4) Verification of performance parameters (temperature, pressure, voltage) regularly; 5) Storage of the instrument in a clean, dry environment when not in use; 6) Keeping a maintenance log to track service activities and identify potential issues early; 7) Following the manufacturer's specific maintenance schedule for optimal performance and longevity.
4. Can this analytical equipment be used with different types of samples, and are there any compatibility considerations?
Yes, this analytical equipment can be used with a variety of sample types, but compatibility considerations are important: 1) Check if the instrument's materials are compatible with the chemical composition of your samples (avoid corrosive or reactive substances that may damage the instrument); 2) Ensure that the sample volume and concentration are within the instrument's recommended range; 3) For biological samples, consider sterility requirements and potential cross-contamination risks; 4) For volatile or hazardous samples, ensure proper ventilation and safety precautions are in place; 5) Consult the manufacturer's documentation for specific sample compatibility guidelines, and perform compatibility tests with new sample types before regular use.
5. What should be done if the analytical equipment malfunctions or produces inconsistent results?
If malfunctions or inconsistent results occur: 1) First, check the instrument's user manual for troubleshooting guidelines specific to the issue; 2) Verify that the instrument is properly calibrated and within its calibration period; 3) Inspect for obvious issues such as loose connections, damaged parts, or incorrect settings; 4) Clean the instrument thoroughly to remove any contamination that may affect results; 5) Repeat the experiment with a known standard sample to determine if the issue is with the instrument or the sample; 6) If the problem persists, discontinue use of the instrument to prevent further damage or inaccurate results; 7) Contact technical support or a qualified service technician for diagnosis and repair, providing details of the issue and any troubleshooting steps already performed.
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