Real Time PCR LRTP-T2012

Introduction

Real Time offers a block temperature range from 0℃ to 105℃, providing precise thermal control. It incorporates a non-fiber optical system, ensuring minimal signal loss. The enhanced signal sensitivity improves detection accuracy. Its temperature gradient function allows for optimized reaction conditions across multiple samples. Our adjustable touchscreen display provides an intuitive interface for easy operation.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Real Time is a molecular biology technique used to amplify and quantify or in real time. It is widely applied in clinical diagnostics, research, and biotechnology for detecting pathogens, gene expression analysis, and mutation detection.

Specifications

Sample Capacity	96 wells × 0.1 ml
Reaction Volume	10 to 50 µl
Block Temperature Range	0℃ to 105℃
Maximum Heating Rate	6℃/sec
Maximum Cooling Rate	5℃/sec
Temperature Uniformity	≤ ±0.2℃ at 90℃
Temperature Accuracy	≤ ±0.1℃
Display Resolution	0.1℃
Heat Lid Temperature Range	30℃ to 112℃
Temperature Control Mode	Block & Calculated Sample
Gradient Range	30℃ to 105℃
Temperature Differential Range	0.1℃ to 42℃
Fluorescence Excitation	Long life lamps
Fluorescence Detection	CCDs
Dynamic Range	1 to 1010
Sensitivity	≥ 1 copy
Calibrated Dyes at Installation	F1: , F2: , , , , CY3, F3: , -, F4: CY5, Quasar670 F5: CY5.5 F6: Reserved
Fluorescence Excitation Range	300 to 800 nm
Fluorescence Detection Range	500 to 800 nm
Tubes Option	96-well plate with optical flat cap
Heating & Cooling Technology	New generation Peltier technology, 1,000,000 cycles
Display	10
Maximum Number of Programs	Maximum 15,000 onboard, unlimited via flash drive
Power Consumption	600 W
Power Supply	100 to 240 V, 50 to 60 Hz
Dimensions (L × W × H)	334 × 280 × 365 mm
Weight	13 kg

Features

High-Speed Thermal Cycling

Accurate Quantification Results

Real-Time Data Analysis

Multiplex Detection Capability

Advanced Optical System

FAQs

1. How can Real-Time system help optimize primer and probe design?

Real-Time systems come with assay optimization guidelines that assist users in designing primers and probes with high specificity and efficiency. The systems support software that recommends optimal primer melting temperatures (Tm), GC content, and amplicon length, minimizing the risk of primer-dimer formation and non-specific amplification. Additionally, technical team provides guidance on assay optimization, ensuring reliable and reproducible results.

2. Can Real-Time system be used for multiplex assays?

Yes, Real-Time systems support multiplex assays, enabling the simultaneous detection of multiple targets in a single reaction. The system is equipped with multiple fluorescence detection channels that allow for the detection of distinct fluorophores, minimizing cross-talk between channels. This feature makes it ideal for applications such as pathogen detection, gene expression analysis, and mutation screening, reducing time and resource consumption.

3. How does Real-Time system ensure high specificity in amplification?

Real-Time systems achieve high specificity by incorporating optimized thermal cycling profiles, primer-probe design validation, and precise temperature control. Additionally, the system’s software supports melt curve analysis to differentiate between specific and non-specific amplification products. These features ensure that target / sequences are accurately amplified, reducing the likelihood of false-positive results.

4. What is the detection sensitivity of Real-Time systems?

Real-Time systems offer high sensitivity, capable of detecting low target copy numbers with minimal background noise. The systems utilize advanced fluorescence detection technology, ensuring accurate and reliable quantification of nucleic acids, even at very low concentrations. This high sensitivity makes systems suitable for applications such as infectious disease diagnostics, gene expression analysis, and environmental monitoring.

5. Does Real-Time system support one-step and two-step RT-?

Yes, Real-Time systems are compatible with both one-step and two-step reverse transcription (RT-) protocols. The systems provide flexibility in analysis by allowing users to choose between these approaches based on their experimental needs. One-step RT- integrates reverse transcription and amplification in a single reaction, ideal for high-throughput applications, while two-step RT- offers more control over individual steps, making it suitable for assay optimization.

6. Can Real-Time system be used for digital (dPCR) applications?

While Real-Time systems primarily perform quantitative (qPCR), some models can be adapted for digital (dPCR) applications with appropriate modifications. Digital provides absolute quantification by partitioning the sample into thousands of individual reactions, enabling precise detection of rare mutations and low-abundance targets. technical team can assist with protocol adaptation for specific dPCR needs.

7. How does Real-Time system handle errors during runs?

Real-Time systems include built-in error detection mechanisms that alert users to potential issues during runs. The systems automatically monitor reaction conditions, fluorescence signals, and temperature variations. In case of errors, the system generates detailed error reports and provides troubleshooting recommendations through the software interface, helping users quickly identify and resolve issues.