Real Time PCR LRTP-T2040

Introduction

Real Time a powerful, precise, modular design thermal cycler system with 4-fluorescence detection channel. Based on advanced Peltier-based technology with high amplification efficiency up to 5°C/s maximum ramp rate saves valuable time. A powerful optical system offers Automatic collection of data from all wells during the detection process using white camera. Tailored with 470 nm, 525 nm, 585 nm, 610 nm Excitation filters, and 525 nm, 570 nm, 610 nm, 678 nm detection filters alongside different fluorescent channels like Channel 1: /, Channel 2: /////CY3, Channel 3: /TexasRed, Channel 4: CY5, for various applications. Independent software analysis module enables easy experiment setup and delivers faster results. Ingenious design with the standard configuration of 96-well plate to carry out the quantitative study in gene expression, genetic variation, genotyping, etc. using small amounts of or as a nucleic acid sample. This device can be customized with an optional 5th channel based on customer requirements.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Real Time widely implemented in vitro method for multiplication/amplification /quantification of fluorescence dye tagged or nucleic acid. Real-time thermal cyclers are used to conduct the quantitative study in gene expression, genetic variation, genotyping, protein expression, etc. using small amounts of or as a nucleic acid sample. machine is in high demand across Genetic Engineering, Virology, Biotechnology, Medicine, Molecular Biology, Forensic Science, and Pharma Industry.

Specifications

Fluorescence Detection Channel	4
Reactions Per Run	96
Block Format	96-well, 0.2 ml
Light Source	High brightness monochrome
Detector	Highly sensitive cold light
Dynamic Detection Range	100 to 1010
Sensitivity	1 copy
Reaction Volume	15 to 100 µl
Excitation Source	White
Excitation Filters	Channel 1: 470 nm, Channel 2: 525 nm, Channel 3: 585 nm, Channel 4: 610 nm
Detection Filters	Channel 1: 525 nm, Channel 2: 570 nm, Channel 3: 610 nm, Channel 4: 678 nm
Fluorescent Dyes	Channel 1: /, Channel 2: /////CY3, Channel 3: /TexasRed, Channel 4: CY5
Block Material	Peltier
Temperature Range	0°C to 99°C
Temperature Accuracy	± 0.1 °C
Temperature Uniformity	± 0.4 °C (10 sec after reaching 95°C), ± 0.2 °C (10 sec after reaching 95°C)
Maximum Ramp Rate	5°C/sec
Gradient Range	30°C to 99°C
Chemistry	All real time based analysis, flexibility without passive reference dye.
PC Operation System	Windows XP/Vista/7/8/10
Machine Operation System	Linux
	A8
Network	/Wi-Fi
Application Available	Gene Expression, Genotyping, Copy Number Variation, Protein Detection, MicroRNA, Pathogen Detection
Dimension (W×D×H)	592×440×280 mm
Weight	15 kg

Features

3D design with well pressure distribution and heat preservation

Unique sliding track convenient for sample operation

Four different fluorescent channels namely, Channel 1: /, Channel 2: /////CY3, Channel 3: /TexasRed, Channel 4: CY5

Advanced Peltier-based technology with high amplification efficiency up to 5°C/s maximum ramp rate

Factor calibration for optical and thermal accuracy

6 mm aluminum alloy, durable design main body

Adjustable footing designed to maintain a steady balance

Linux operation system, equipped with A8 for better user experience

Connection with PC through Wi-Fi or

Low noise operation, low energy consumption, long life span

Complied with Good laboratory practice standards like 21 part 11

FAQs

1. What are the throughput and detection capabilities of the Real Time ?

The Real Time is designed with a standard block format that allows the simultaneous analysis of multiple samples within a single run, making it suitable for high-efficiency laboratory operations. It integrates four fluorescence detection channels, enabling multiplex detection of different targets in one experiment. The optical system utilizes a white excitation source with a highly sensitive camera, ensuring reliable signal capture from every well. Its broad dynamic detection capacity ensures that a wide range of nucleic acid concentrations can be quantified with precision. Together, these features make the Real Time an excellent choice for laboratories seeking accuracy and versatility in applications.

2. How does the Real Time ensure precise thermal cycling performance?

The Real Time is powered by advanced Peltier-based block technology that provides stable and consistent temperature control during amplification cycles. Its high ramp rate allows rapid heating and cooling while maintaining thermal accuracy, thereby reducing experiment time. Factor calibration ensures both optical and thermal accuracy, giving researchers confidence in the reproducibility of their results. The system’s uniform temperature distribution across the block prevents variability between wells. Overall, the Real Time delivers precise and dependable thermal cycling for a wide variety of applications.

3. What optical and detection technologies are integrated in the Real Time ?

The Real Time features a white as its excitation light source and a highly sensitive cold light as its detector. It is configured with multiple excitation and detection filters that support a wide range of fluorescent dyes including , , , , , and CY5. This multi-channel capability allows researchers to perform diverse applications such as gene expression studies, mutation analysis, and genotyping. Its optical design ensures minimal background interference and highly accurate fluorescence signal capture. As a result, the Real Time provides robust and consistent detection across various experimental workflows.

4. What software and user interface features does the Real Time provide?

The Real Time is designed with an independent analysis module that allows simple experiment setup and fast result generation. Its system is supported by a Linux operating environment powered with an A8 for smooth performance. Additionally, it offers connectivity to PC software via Wi-Fi or , giving researchers flexible options for remote operation and data management. The software includes tools for quantitative analysis, genotyping, and copy number variation studies, enabling versatile applications. With its user-friendly design and reliable interface, the Real Time ensures both ease of use and advanced functionality.

5. In which applications can the Real Time be effectively used?

The Real Time is highly adaptable and serves multiple domains in scientific research and applied diagnostics. It is commonly employed in genetic engineering, biotechnology, molecular biology, forensic science, and pharmaceutical research. Researchers use it for studies involving gene expression, genetic variation, microRNA analysis, and pathogen detection. Its design also supports applications in protein detection and copy number variation studies, broadening its utility across disciplines. In essence, the Real Time provides researchers with a dependable tool for precise nucleic acid quantification and molecular analysis.