Vertical Electrophoresis System LVES-T2011

Introduction

Vertical Electrophoresis System offers gel dimensions of 186 × 205 mm, providing ample space for reliable and consistent gel electrophoresis. It ensures leak-proof gel casting, preventing sample leakage and ensuring high accuracy. The system guarantees uniform band resolution, enabling precise visualization and analysis of nucleic acids and proteins. It supports versatile gel thickness options, allowing users to choose the appropriate thickness for specific applications. Our system has an efficient buffer circulation system that maintains uniform conditions throughout the process, ensuring optimal performance.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Vertical Electrophoresis System  is used to separate proteins or nucleic acids based on their size and charge through a vertical gel matrix. It is used in molecular biology, and clinical research for protein analysis, / separation, and genetic studies.

Specifications

Glass Plate Dimensions	216 × 220 mm
Gel Dimensions	186 × 205 mm
Gel Thickness	1.0 mm
Number of Gels	1 to 2 pcs
Maximum Buffer Volume	3500 ml
Sample Throughput	25 to 104
Dimension (L × W × H)	290 × 170 × 250 mm
Weight	6.2 kg

Features

Fast Gel Plate Installation

Secure Lid Button Design

Consistent Cooling and pH

High-Resolution Gel Separation

Multi-Gel Preparation Capability

FAQs

1. Does the system support gradient gels for protein separation?

Yes, Vertical Electrophoresis System supports the use of gradient gels, which provide superior resolution for complex protein mixtures by offering a gradual change in polyacrylamide concentration. Gradient gels allow for the separation of proteins over a wide molecular weight range, enhancing the resolution of closely migrating bands. It offers high-quality gel casting accessories that enable users to create custom gradient gels with precision and consistency.

2. What types of fluorescence dyes are compatible with Real-Time System?

Real-Time System is compatible with a wide range of fluorescent dyes and probes commonly used in qPCR applications. These include Green, TaqMan probes, EvaGreen, and other intercalating dyes or hydrolysis probes. system is equipped with advanced optical detection technology that ensures high sensitivity and accuracy across multiple fluorescence channels. The system supports multiplexing capabilities, allowing researchers to detect and quantify multiple targets simultaneously within the same reaction, thereby increasing experimental efficiency.

3. Can Real-Time System handle multiplex reactions?

Yes, Real-Time System is specifically designed to handle multiplex reactions, enabling the simultaneous detection of multiple target sequences in a single reaction. The system’s high-resolution optical detection allows for discrimination between different fluorophores, ensuring accurate quantification even in complex samples. software also provides tools for optimizing primer-probe designs and analyzing multiplex reactions, making it easier for researchers to perform high-throughput assays efficiently.

4. How does the system prevent cross-contamination during qPCR runs?

Real-Time System is designed with advanced contamination control mechanisms to minimize the risk of cross-contamination. The system’s enclosed design, combined with a sealed reaction environment, prevents aerosol or sample carryover between runs. Additionally, It recommends using (Uracil-N-Glycosylase) to degrade any previously amplified that may contaminate the reaction mix, further reducing the possibility of false positives.

5. What factors should be considered while designing primers and probes for system?

When designing primers and probes for Real-Time System, it is essential to consider melting temperature (Tm), GC content, amplicon length, and specificity to ensure efficient and accurate amplification. Primers should have a Tm between 55°C and 65°C, with a GC content of 40-60%, while amplicon lengths should ideally range between 80-200 bp for optimal performance. It provides detailed guidelines and software tools to assist in designing high-quality primers and probes, ensuring minimal non-specific amplification and maximum efficiency.

6. How does uniform temperature distribution during qPCR runs?

Real-Time System uses advanced Peltier heating and cooling technology to ensure precise and uniform temperature distribution across all wells during qPCR runs. The system’s thermal block design minimizes temperature variation, ensuring consistent amplification and reliable quantification of target sequences. real-time temperature monitoring further guarantees that the system maintains optimal conditions throughout the entire reaction, reducing variability between runs.

7. Can the system perform gradient for protocol optimization?

Yes, Real-Time System supports gradient functionality, allowing users to optimize reaction conditions by testing different annealing temperatures simultaneously. Gradient is particularly useful for primer optimization and troubleshooting of non-specific amplification. intuitive software provides easy-to-use controls for setting gradient ranges and analyzing results, enabling researchers to fine-tune their protocols for maximum efficiency.