Real Time PCR LRTP-T2011

Introduction

Real-Time with a reaction capacity ranging from 10 to 50 µl, accommodates diverse sample volumes. It features high-speed thermal cycling, enabling rapid amplification. The system delivers accurate quantification results for quantitative analysis. Its multiplex detection capability allows simultaneous analysis of multiple targets. Our real-time data analysis provides immediate insights into reaction progress.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Real Time is a molecular biology technique used to amplify and quantify or in real time. It is widely applied in clinical diagnostics, research, and biotechnology for detecting pathogens, gene expression analysis, and mutation detection.

Specifications

Sample Capacity	96 wells × 0.1 ml
Reaction Capacity	10 to 50 µl
Block Temperature Range	0℃ to 105℃
Maximum Heating Rate	6℃/sec
Maximum Cooling Rate	5℃/sec
Temperature Uniformity	≤ ±0.2℃ at 90℃
Temperature Accuracy	≤ ±0.1℃
Display Resolution	0.1℃
Heat Lid Temperature Range	30℃ to 112℃
Temperature Control Mode	Block & Calculated Sample
Gradient Range	30℃ to 105℃
Temperature Differential Range	0.1℃ to 42℃
Fluorescence Excitation	Long life lamps
Fluorescence Detection	CCDs
Dynamic Range	1 to 10¹⁰
Sensitivity	≥ 1 copy
Calibrated Dyes at Installation	F1: , F2: , , , , CY3, F3: , -, F4: CY5, Quasar670
Fluorescence Excitation Range	300 to 800 nm
Fluorescence Detection Range	500 to 800 nm
Tubes Option	96-well plate with optical flat cap
Heating & Cooling Technology	New generation Peltier technology, 1,000,000 cycles
Display	10
Maximum Number of Programs	Maximum 15,000 onboard, unlimited via flash drive
Power Consumption	600 W
Power Supply	100 to 240 V, 50 to 60 Hz
Dimensions (L × W × H)	334 × 280 × 365 mm
Weight	13 kg

Features

High Signal Sensitivity

Fast Protocol Execution

Efficient Temperature Gradient

Dust-Resistant Optical System

Enhanced Fluorescence Detection

FAQs

1. What are the thermal cycling capabilities of Real-Time systems?

Real-Time systems are equipped with advanced Peltier heating and cooling technology, ensuring rapid and uniform thermal cycling. The systems offer a broad temperature range with precise control and minimal temperature variation across the sample block, enabling high efficiency in denaturation, annealing, and extension steps. This ensures reliable and consistent amplification, making the system suitable for a wide range of assays, including standard, fast, and high-resolution protocols.

2. Does Real-Time system support multiple reaction formats?

Yes, Real-Time systems are compatible with various reaction formats, including 96-well, 384-well plates, and strips. The system can accommodate different reaction volumes, allowing users to customize assay conditions based on their experimental requirements. This flexibility makes it suitable for both low-throughput research studies and high-throughput diagnostic applications, offering seamless adaptability across diverse laboratory settings.

3. Can Real-Time system perform high-resolution melting () analysis?

Yes, Real-Time systems offer High-Resolution Melting () analysis capabilities, which allow for the detection of subtle sequence variations, such as single nucleotide polymorphisms (SNPs) and methylation changes. The system’s high-resolution optical detection and advanced data analysis software provide accurate melting curve analysis, enabling the differentiation of sequences with high precision. analysis is particularly useful for applications such as mutation scanning, genotyping, and epigenetic studies.

4. How does the accuracy and reproducibility of Real-Time results?

Real-Time systems ensure high accuracy and reproducibility through optimized thermal cycling profiles, precise fluorescence detection, and robust quality control processes. The systems undergo rigorous calibration and validation procedures to minimize variability between runs and across different instruments. Additionally, It provides comprehensive assay optimization guidelines and technical support to help users achieve consistent and reliable results.

5. What measures does to prevent cross-contamination in Real-Time systems?

Real-Time systems are equipped with contamination prevention features such as sealed reaction chambers, UV sterilization, and filtration to minimize the risk of cross-contamination. Additionally, the system’s automated workflow reduces manual handling of samples, further preventing contamination. These features ensure the integrity of assay results, particularly in clinical and diagnostic applications where accuracy is critical.

6. Does temperature gradient functionality in its Real-Time systems?

Yes, Real-Time systems feature temperature gradient functionality, allowing users to optimize annealing temperatures across different zones of the reaction block. This feature is particularly useful during assay development, enabling researchers to determine the ideal conditions for maximum amplification efficiency. By running gradient , users can fine-tune reaction parameters and improve overall assay performance.

7. Can Real-Time system be used for environmental monitoring and microbial detection?

Yes, Real-Time systems are well-suited for environmental monitoring and microbial detection, allowing for the identification and quantification of microorganisms in water, soil, and air samples. The systems offer high sensitivity and multiplexing capabilities, enabling the detection of multiple pathogens in a single assay. Their robust design and accurate detection make them ideal for use in environmental research, public health monitoring, and regulatory compliance.