Real Time PCR LRTP-T2010

Introduction

Real Time has a capacity of 96 wells × 0.1 ml and efficiently handles high-throughput analysis. It provides high signal sensitivity, ensuring precise data acquisition. The system enables fast protocol execution, reducing testing time. Its efficient temperature gradient ensures uniform heat distribution. Our has enhanced fluorescence detection, improving signal clarity for complex quantitative and qualitative analysis.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Real Time is a molecular biology technique used to amplify and quantify or in real time. It is widely applied in clinical diagnostics, research, and biotechnology for detecting pathogens, gene expression analysis, and mutation detection.

Specifications

Sample Capacity	96 wells × 0.1 ml
Reaction Capacity	10 to 50 µl
Block Temperature Range	0℃ to 105℃
Maximum Heating Rate	6℃/sec
Maximum Cooling Rate	5℃/sec
Temperature Uniformity	≤ ±0.2℃ at 90℃
Temperature Accuracy	≤ ±0.1℃
Display Resolution	0.1℃
Heat Lid Temperature Range	30℃ to 112℃
Temperature Control Mode	Block & Calculated Sample
Gradient Range	30℃ to 105℃
Temperature Differential Range	0.1℃ to 42℃
Fluorescence Excitation	Long life lamps
Fluorescence Detection	CCDs
Dynamic Range	1 to 10¹⁰
Sensitivity	≥ 1 copy
Calibrated Dyes at Installation	F1: , F2:, , , , CY3,
Fluorescence Excitation Range	300 to 800 nm
Fluorescence Detection Range	500 to 800 nm
Tubes Option	96-well plate with optical flat cap
Heating & Cooling Technology	New generation Peltier technology, 1,000,000 cycles
Display	10
Maximum Number of Programs	Maximum 15,000 onboard, unlimited via flash drive
Power Consumption	600 W
Power Supply	100 to 240 V, 50 to 60 Hz
Dimensions (L × W × H)	334 × 280 × 365 mm
Weight	13 kg

Features

Non-Fiber Optical System

Enhanced Signal Sensitivity

Temperature Gradient Function

Adjustable Touchscreen Display

Simultaneous Multi-Well Detection

FAQs

1. How does a Real-Time quantify or ?

Real-Time quantifies or by measuring the fluorescence emitted during each cycle of amplification. The intensity of the fluorescence signal increases proportionally with the amount of target generated, allowing the system to quantify the nucleic acid concentration in real time. Real-Time systems use high-precision optical detection systems that monitor fluorescence signals accurately, enabling reliable quantification of nucleic acids across a wide dynamic range. The software automatically generates standard curves for absolute or relative quantification.

2. Can Real-Time be used for multiplexing?

Yes, Real-Time can perform multiplexing, where multiple target sequences are amplified and detected simultaneously in a single reaction. Multiplexing uses different fluorescent dyes or probes for each target, allowing for the detection of multiple genes or pathogens in one assay. Real-Time systems support multiplexing capabilities with high fluorescence channel sensitivity, ensuring accurate and reproducible results even in complex sample matrices. This feature enhances efficiency and reduces the time and cost associated with running multiple tests.

3. What is the sensitivity and detection limit of a Real-Time system?

The sensitivity and detection limit of a Real-Time system depend on factors such as assay design, template quality, and reaction conditions. high-quality qPCR systems can detect as few as 1–10 copies of the target or . Real-Time systems offer high sensitivity and a broad dynamic range, ensuring reliable detection of low-abundance targets with minimal background noise. The system’s optimized thermal cycling protocols and fluorescence detection enhance assay sensitivity and reproducibility.

4. Can Real-Time be used for quantifying gene expression?

Yes, Real-Time is a powerful tool for quantifying gene expression by measuring mRNA levels after reverse transcription to cDNA. Real-Time systems are compatible with one-step or two-step reverse transcription protocols, ensuring efficient conversion of to cDNA. With advanced software for relative quantification using reference genes and normalization, systems provide accurate and reproducible gene expression analysis for various applications, including biomarker discovery and drug development.

5. Can Real-Time be used for pathogen detection in clinical samples?

Yes, Real-Time is extensively used for pathogen detection in clinical samples, including bacteria, viruses, and fungi. It is particularly effective for detecting infectious agents such as -CoV-2, , and with high sensitivity and specificity. Real-Time systems are equipped with rapid thermal cycling and multiplexing capabilities, enabling the simultaneous detection of multiple pathogens from a single sample. Their accuracy and reliability make them suitable for routine clinical diagnostics and epidemiological surveillance.

6. Can Real-Time be used for mutation detection and genotyping?

Yes, Real-Time can be used for mutation detection and (Single Nucleotide Polymorphism) genotyping by employing allele-specific probes or melting curve analysis. Real-Time systems offer high-resolution melting () analysis and TaqMan genotyping assays that enable precise differentiation of alleles and mutations. These features are essential for applications such as cancer research, personalized medicine, and pharmacogenomics, where detecting genetic variations is critical.

7. Can Real-Time be used in food safety and detection?

Yes, Real-Time is a highly effective tool for food safety testing and detection, enabling the identification of genetically modified organisms, pathogens, and allergens in food products. Real-Time systems provide high sensitivity and specificity in detecting target sequences from food samples. The system’s rapid cycling and multiplexing capabilities allow for the simultaneous detection of multiple targets, ensuring compliance with international food safety standards and regulations.