Real Time PCR LRTP-T2042

Introduction

Real Time is a fluorescent quantitative system with a throughput of 96 wells and supports up to 6 channels. Offers a gradient temperature control range of 30 to 100°C with a maximum temperature difference of 40°C. Features automatic movement of the sample tray, facilitating integration with automated workstations and plate handlers. Our system combines an innovative thermal cycling system with precise photoelectric detection and advanced software.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Our real-time is used in gene expression analysis, microbial quantification, genetic variation analysis, viral load measurement, and forensic analysis.

Specifications

Fluorescence Detection Channels	6 channels
Reaction Per Run	96
Throughput	96well × 0.2ml (half-skirted/no-skirted plate)
Block Compatibility	96 well Plates, 12- well strips tube, and 8- well strip tube.
Programmable Span	999 cycles
Dynamic Range	1 to 10¹⁰copies
Detection Location	Top detection
Single Channel Scan Time	33s
Excitation Light Wavelength	400 to 800nm
Detection Wavelength	500 to 800nm
Fluorochrome Dye	Channel 1: / Green I Channel 2: ///IC Channel 3: //Cy3 Channel 4: /Texas Red Channel 5: Cy5 Channel 6: Cy5.5
Excitation Light Source	full-spectrum
Detector
Block Material	Peltier
Block Temperature Range	4 to 105°C
Block Temperature Accuracy	≤ ± 0.1°C
Block Temperature Precision	≤ ± 0.1℃
Block Temperature Uniformity	≤ ± 0.1℃
Block Ramping Rate	≥ 6 ℃/S
Temperature Control Mode	Block mode, Tube mode
Gradient Temperature Difference	1 to 40°C
Sample Volume Range	5 to 100 µL
Heated Lid Temperature Range	30°C to 110°C (default 105°C)
Fluorescence Intensity	CV ≤ 3%
Detection Repeatability	Yes
Display	10.1-inch touch screen
Scan Mode	Full plate scan or specified line scan
Software Functions	Absolute quantification, Relative quantification, Melting curve, genotyping, , Quick run, etc.
Operation System	PC software (Windows 8 or higher), Built-in software
Connectivity	Type-A port × 2, Type-B port, RJ45 port
Power-Off Protection Function	.
Dimensions (W× L × H)	320 × 525 × 420 mm
Net Weight	27 Kg
Gross Weight	50 Kg

Features

10.1-inch high-definition color touchscreen

Capable of restoring data after power on

Highly sensitive detection system

Built-in analysis software for easy operation

Temperature control for block or tube

It supports qPCR (Real-Time )

Utilizes a full-spectrum high-power

Highly sensitive photomultiplier tube

Designed ergonomically for user comfort

FAQs

1. What are the throughput and detection capabilities of the Real Time ?

The Real Time is designed to support large-scale laboratory testing needs by accommodating multiple samples in each run, making it ideal for high-volume workflows. It is equipped with several fluorescence detection channels that enable simultaneous analysis of multiple targets in a single reaction, which enhances efficiency. The wide detection capacity ensures both low-abundance and high-abundance genetic materials can be reliably identified. By incorporating a top detection system, the instrument minimizes background noise and delivers highly sensitive results. Together, these capabilities ensure that the Real Time consistently produces accurate and reproducible outcomes across a wide range of applications.

2. How does the Real Time ensure precise thermal cycling performance?

The Real Time incorporates an advanced Peltier block system that delivers stable and precise temperature control during every amplification cycle. Its ability to maintain highly accurate temperature settings ensures the integrity of and amplification processes. With rapid heating and cooling capabilities, the instrument enables faster cycling while maintaining precision. A gradient temperature feature allows users to optimize annealing conditions across various assays, improving experimental flexibility. Altogether, the Real Time ensures that researchers achieve reliable and reproducible results in every run.

3. What optical and detection technologies are integrated in the Real Time ?

The Real Time employs a full-spectrum excitation system paired with a highly sensitive photomultiplier tube detector for maximum accuracy. Its broad excitation and detection capability allow it to work with a wide variety of fluorescent dyes commonly used in molecular biology. By supporting multiple dye channels, the instrument enables applications such as absolute quantification, relative quantification, melting curve analysis, and genotyping. High sensitivity ensures consistent detection performance, even when working with challenging samples. Overall, the Real Time combines robust optical engineering with advanced detection technology to deliver precise and reproducible results.

4. What software and user interface features does the Real Time provide?

The Real Time is equipped with a large high-definition touchscreen that offers intuitive operation and clear visibility during experiments. Its compatibility with PC-based software allows users to seamlessly manage and analyse data across different platforms. The built-in software provides advanced analysis modes, including absolute and relative quantification, melting curve studies, and genotyping applications. The instrument also offers flexibility in scanning options, enabling both full plate scans and selective region scans depending on workflow needs. With its automated tray movement and user-friendly interface, the Real Time simplifies operation while maintaining advanced analytical capabilities.

5. In which applications can the Real Time be effectively used?

The Real Time is a highly versatile tool suitable for a broad range of research and diagnostic applications. It is frequently used in genetic studies, microbial analysis, viral detection, and forensic investigations where sensitivity and accuracy are essential. The instrument supports laboratories in conducting both basic research and applied testing with equal reliability. Its compatibility with automated systems makes it particularly well-suited for high-throughput environments where efficiency is critical. In summary, the Real Time serves as a dependable and efficient solution for molecular biology, clinical diagnostics, and life sciences research.