Pulsed Field Gel Electrophoresis System LPFGES-T2010

Introduction

Pulsed Field Gel Electrophoresis System gel dimensions of 120 × 120 mm for routine electrophoresis applications requiring moderate throughput. It allows for efficient sample loading, ensuring minimal handling time and increased workflow productivity. It features programmable run modes that offer flexibility for various protocols. The safe electrode shielding ensures operator protection and minimizes the risk of electrical hazards. Our pulsed field gel electrophoresis system has low power consumption, making it energy-efficient without compromising performance.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Pulsed Field Gel Electrophoresis System is a technique used to separate large molecules by applying an alternating electric field. It is widely used in microbiology, epidemiology, and genetic research for bacterial strain typing and genome mapping.

Specifications

Gel Dimensions (W × L)	120 × 120 mm
Buffer Volume	3000 ml
Sample Capacity	9 to 13
Temperature Range	10℃ to 30℃, Tolerance <±0.5℃
Temperature Detection	<±0.1°C
Separation Fragment	100 bp to 6 Mb
Voltage Gradient	0.1 V/cm to 9.5 V/cm
Maximum Current	0.5 A
Pulse Angle	±120°
Pulse Width	1 to 9999 secs programmed pulse or 1 to 20 s of line-increase pulse
Module Timing	1 min to 99 hrs 59 mins
Power Consumption	300 W
Power Supply Dimensions	280 × 240 × 110 mm
Pulse Tank Dimensions	465 × 445 × 142 mm
Output Control Allocation Dimensions	465 × 445 × 100 mm
Constant Temperature Circulator	350 × 200 × 480 mm
Net Weight	50 kg
Gross Weight	69 Kg

Features

High-Resolution Separation

Uniform Pulse Control

Advanced Cooling System

Reliable Fragmentation

Consistent Band Sharpness

FAQs

1. What is a Pulsed Field Gel Electrophoresis () system used for?

A system is used to separate extremely large molecules, often in the range of 50 kb to over 10 Mb, which are too large to resolve using standard agarose gel electrophoresis. This technique is essential in fields like molecular epidemiology, bacterial strain typing, and genome structure analysis. For example, it's commonly used in tracking outbreaks of foodborne pathogens such as E. coli or Salmonella. It provides systems with robust gel units and programmable pulse controllers that allow precise manipulation of the electric field direction, enabling high-resolution separation of large fragments necessary for complex molecular investigations.

2. How does Pulsed Field Gel Electrophoresis differ from conventional agarose gel electrophoresis?

differs fundamentally in the way it applies the electric field. While conventional electrophoresis uses a constant unidirectional field, applies a periodically switching field at different angles (usually 120°), allowing large molecules to reorient themselves and snake through the gel. This method improves the resolution of molecules that would otherwise migrate together. systems feature advanced pulse controllers that let users set parameters like pulse interval, switch angle, and voltage gradients, giving researchers full control over the separation process for a wide range of sizes.

3. What types of samples are analyzed using Pulsed Field Gel Electrophoresis?

is most commonly used to analyze large genomic , including chromosomal from bacteria, yeast, fungi, or eukaryotic organisms. It is especially valuable for comparing bacterial strains during disease outbreak investigations and for genome mapping in molecular biology. systems are optimized to work with plug-embedded , maintaining integrity during the lysis and digestion steps. This approach ensures minimal shearing and supports high-fidelity results, which are essential in clinical diagnostics, food safety monitoring, and public health labs.

4. What are the safety considerations when using a Pulsed Field Gel Electrophoresis system?

Safety is a key concern when working with systems due to the high voltage used and the common use of UV light and staining chemicals. It incorporates multiple safety features into its systems, such as interlocking lids that prevent exposure to live currents, overload protection circuits, and automated shut-off functions in case of malfunctions. These ensure that the system can be operated safely in laboratory environments, even during prolonged runs. Additionally, optional blue-light imaging minimizes the use of UV exposure, promoting a safer workflow for users handling visualization.

5. What type of buffer is used in Pulsed Field Gel Electrophoresis, and how does it affect the results?

The most commonly used buffer in is 0.5X (Tris-Borate-), which provides optimal ionic strength and buffering capacity to facilitate the smooth migration of large fragments. The buffer also helps in maintaining a stable pH and reducing heat generation during long electrophoresis runs. The quality and composition of the buffer are crucial for maintaining integrity and ensuring consistent migration. systems are designed with efficient buffer circulation and temperature control, which minimizes buffer degradation and helps maintain optimal electrophoresis conditions throughout extended runs, ensuring clear and reproducible band patterns.