Introduction
An anaerobic jar is a laboratory container used to create and maintain an anaerobic environment for the culture of anaerobic microorganisms, which grow only in the absence of oxygen, and it is used in microbiology laboratories for the cultivation and study of anaerobic bacteria. The anaerobic jar consists of a sealed jar or container with a lid, and it is used with an anaerobic gas-generating system, such as an anaerobic gas pack or an envelope that, when activated, generates hydrogen and carbon dioxide; the hydrogen combines with the oxygen in the jar, in the presence of a catalyst, typically palladium, to form water, removing the oxygen and creating an anaerobic environment, and a redox indicator, such as methylene blue, is placed in the jar to confirm the anaerobic conditions, and the inoculated culture plates or tubes are placed in the jar, which is then sealed and incubated at the appropriate temperature; the anaerobic jar provides a simple and convenient method for anaerobic culture without the need for an anaerobic chamber, and the atmosphere in the jar is monitored with the indicator. Anaerobic jars are used in clinical and research microbiology laboratories for culturing anaerobic bacteria from clinical specimens, for studying anaerobic microorganisms, for antibiotic susceptibility testing of anaerobes, for quality control in food microbiology, and for many other applications, and they are available in various sizes, with features such as sealed lids, gas pack holders, and indicator clips. With their provision of an anaerobic environment, anaerobic jars are essential for anaerobic culture in microbiology.
Anaerobic Jar LAJ-T2010
Anaerobic Jar with a portable, lightweight design and a 1.5 clamp, providing strong and reliable securement. This lab products is designed to meet the rigorous requirements of modern lab...
Anaerobic Jar LAJ-T2011
Anaerobic jar has a pumping time of 3 to 5 minutes each time and a capacity of 2.5L for maintaining an oxygen-free environment. It comes with a snap-shut coupling for easy system connect...
Anaerobic Jar LAJ-T2013
Anaerobic Jar a 6L capacity and is engineered to maintain optimal anaerobic conditions. It features a robust design that resists physical impacts and temperature changes, ideal for long-...
Anaerobic Jar LAJ-T2012
Anaerobic Jar a capacity of 3.5L and requires 3 to 5 minutes of pumping time peruse to ensure optimal anaerobic conditions. It features a high-quality O-ring that ensures a tight seal be...
Anaerobic Jar LAJ-T2010
Anaerobic Jar is a pumping type and takes 3 to 5 minutes for air evacuation. It is equipped with advanced another side tube for evacuation and of gases. This unit features a...
Anaerobic Jar LAJ-T2011
Anaerobic Jar comes with a hermetic-type design and contains 3 stacks of 8 dishes (ф9-10 cm) for cultivating anaerobic bacteria. It features a lightweight design with convenient portabi...
Anaerobic Jar LAJ-T2020
Anaerobic Jar contents include a single stack of 12 dishes (ɸ90 to 100 mm), with a time taken of 4 hours for optimal anaerobic conditions. It features material with an extremely smooth ...
Standards
Complies with the above standards, but is not limited to them.
Features
1. Anaerobic jar for creating anaerobic atmosphere for culturing anaerobic microorganisms.
2. Sealed jar with gas pack or evacuation-replacement system for anaerobic incubation.
3. Suitable for microbiology, clinical, food, environmental, pharmaceutical and research labs.
4. Includes sealed jar, lid with catalyst, gas pack holder, pressure indicator and operation manual.
5. Supports anaerobic culture incubation for isolation and identification of anaerobes.
FAQ
Q1: What testing scenarios is this jar mainly applied to?
A: The anaerobic jar is used in microbiology laboratories, clinical diagnostics, food testing, environmental monitoring, pharmaceutical QC, water testing and research to create an anaerobic (oxygen-free) or microaerophilic atmosphere inside a sealed jar for culturing anaerobic, microaerophilic or capnophilic microorganisms for isolation, identification and susceptibility testing. It supports clinical microbiology anaerobic culture (isolation of Clostridium, Bacteroides, Fusobacterium, Peptostreptococcus from clinical specimens), food microbiology anaerobic testing (Clostridium perfringens, Clostridium botulinum in food), environmental anaerobic culture (soil, water, sediment samples), pharmaceutical QC anaerobic testing (sterility testing, microbial limits), water testing (sulfate-reducing bacteria, anaerobic coliforms), and research on anaerobic microorganisms. The anaerobic jar provides a convenient, compact method for creating anaerobic conditions without requiring an anaerobic chamber (glove box), suitable for low-to-medium volume anaerobic culture work in clinical and research laboratories.
Q2: What standards does the jar comply with?
A: The anaerobic jar follows relevant ISO, ASTM, CLSI and GB/T standards for anaerobic culture, including ISO 14461 (milk and milk products - anaerobic spore-forming bacteria), ISO 7937 (Clostridium perfringens enumeration), ASTM standards for anaerobic culture, CLSI M40 (quality control for antimicrobial susceptibility testing, including anaerobes), and relevant GB/T standards for food and clinical microbiology. The hardware complies with applicable safety standards for pressure vessels (the jar is under slight positive pressure during anaerobic gas generation). Gas packs (gas generating sachets) should be from qualified suppliers per standard. Catalyst (palladium-coated alumina pellets) should be active and properly maintained. Users should verify the specific standard for their application (clinical, food, environmental).
Q3: What is the warranty period for the whole jar and key components?
A: The whole anaerobic jar is covered by a 12-month warranty. The jar body (polycarbonate or stainless steel), lid, sealing gasket, catalyst holder and pressure indicator are covered, while gaskets, catalysts (palladium), gas packs, O-rings and pressure indicators are consumables or accessories. Damage from improper use, dropping, gasket damage, overpressure or unauthorized repair is excluded. Extended warranty may be available. Polycarbonate jars can become cloudy or brittle over time (especially with repeated autoclaving - check manufacturer autoclave rating).
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained microbiology laboratory personnel familiar with anaerobic culture techniques, aseptic technique and biosafety. We provide operation manuals, training and application support. Proper jar preparation (clean, dry, gasket in good condition), catalyst activation (palladium catalyst should be active - reactivate by heating if deactivated), gas pack selection (anaerobic, microaerophilic, capnophilic - select based on organism requirements), inoculation and loading (plates should be inoculated before loading, do not overload), sealing and incubation (proper temperature, typically 35-37 degrees C for clinical anaerobes), result interpretation and biosafety procedures are important for successful anaerobic culture.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: No formal calibration required, but regular performance verification is essential: catalyst activity check (use methylene blue or resazurin anaerobic indicator strip in each jar run - if indicator does not turn colorless/white, the atmosphere is not anaerobic; reactivate or replace catalyst), gasket inspection (cracks, wear, deformation - replace if damaged; a faulty gasket causes leaks and fails to maintain anaerobic atmosphere), pressure indicator check (verify it moves/functions properly), jar body inspection (cracks, cloudiness for polycarbonate - replace if damaged), and routine verification with an anaerobic indicator organism (e.g., Clostridium sporogenes or Bacteroides fragilis) per schedule. Full jar performance verification annually or when issues arise. Catalyst replacement/reactivation per manufacturer (typically every 6-12 months or when indicator shows failure - palladium catalyst can be deactivated by sulfur compounds, water, or age; reactivate by heating at 160 degrees C for 2 hours, or replace).
Q7: Are supporting consumables universal?
A: Gas packs (anaerobic gas generating sachets - BD GasPak, Oxoid AnaeroGen, or equivalent), catalysts (palladium-coated alumina pellets), anaerobic indicators (methylene blue, resazurin strips), gaskets, O-rings and cleaning supplies are available from microbiology suppliers, though gas pack type should match the jar size and desired atmosphere. Long-term consumable cost includes gas packs (one per jar run) and catalyst replacement/reactivation.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes jar size (capacity in liters or number of plates - typically holds 10-100 Petri dishes), material (polycarbonate for light weight/transparency, stainless steel for durability/autoclavability), atmosphere type (anaerobic, microaerophilic, capnophilic - determined by gas pack selection), catalyst type, pressure indicator type, rack/plate holder configuration and accessories. Additional racks, gas packs and catalysts can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: No installation is required for anaerobic jars. The package includes the jar, lid, catalyst holder and instruction manual. On-site training is available at additional cost for clinical microbiology setup.
Q10: What types of cultures are compatible with this equipment?
A: The anaerobic jar incubates anaerobic microorganisms (obligate anaerobes: Clostridium, Bacteroides, Fusobacterium, Peptostreptococcus, Prevotella, Porphyromonas; aerotolerant anaerobes), microaerophilic microorganisms (Campylobacter, Helicobacter - require reduced oxygen, typically 5-10% O2), and capnophilic microorganisms (Neisseria, Streptococcus pneumoniae - require increased CO2, typically 5-10% CO2) on agar plates or in broth tubes within the jar capacity, atmosphere type and specification. Different organisms require specific atmospheres: obligate anaerobes require 0% O2, 5-10% CO2, balance N2/H2 (use anaerobic gas pack); microaerophiles require 5-10% O2, 5-10% CO2 (use microaerophilic gas pack); capnophiles require 5-10% CO2 in air (use CO2 gas pack or candle jar, though candle jar is less precise). Users should select appropriate gas pack for the target organism (anaerobic, microaerophilic, capnophilic - check gas pack specifications for O2 and CO2 percentages), use active catalyst (palladium catalyst combines H2 from gas pack with residual O2 to form water, removing oxygen - catalyst MUST be active; deactivated catalyst causes anaerobic failure - verify with indicator strip), include an anaerobic indicator in each jar run (methylene blue strip turns colorless when anaerobic, resazurin turns white - if indicator remains colored, the atmosphere is not anaerobic and cultures may not grow; do not report results from a failed jar run), inoculate plates properly (use fresh cultures, proper inoculation technique - for clinical specimens, inoculate immediately after collection; anaerobes are sensitive to oxygen exposure - minimize oxygen exposure during specimen handling and inoculation), load plates properly (inoculated plates should be placed inverted (agar side up) in the jar, do not overload - leave space for gas circulation, typically 10-50 plates depending on jar size), seal the jar properly (ensure gasket is clean and in good condition, tighten lid evenly, verify pressure indicator shows proper seal), incubate at proper temperature (35-37 degrees C for most clinical anaerobes, 42 degrees C for Campylobacter, 25-30 degrees C for some environmental anaerobes - verify per organism), incubate for proper duration (anaerobes grow slowly - typically 48-72 hours for primary isolation, some slow-growing anaerobes may require 5-7 days; do not open jar too early - opening exposes cultures to oxygen and may kill obligate anaerobes), use appropriate media (anaerobes require enriched media: blood agar (supplemented with hemin, vitamin K1), Brucella blood agar, CDC anaerobe blood agar, Schaedler agar; use selective media for specific organisms: Bacteroides bile esculin (BBE) agar for Bacteroides fragilis group, kanamycin-vancomycin laked blood (KVLB) agar for Prevotella/Porphyromonas, cycloserine-cefoxitin-fructose agar (CCFA) for Clostridioides difficile), and for clinical use, follow proper anaerobic culture protocols (CLSI guidelines, include appropriate controls: anaerobic control organism such as Bacteroides fragilis ATCC 25285, aerobic control to verify anaerobic conditions - an obligate aerobe should not grow in a properly functioning anaerobic jar). The jar should be kept clean and dry between uses (residual moisture can affect gasket seal and catalyst activity; clean with mild detergent, rinse, dry completely). The gasket should be inspected regularly and replaced when worn (a faulty gasket is the most common cause of anaerobic jar failure). The catalyst should be reactivated or replaced per schedule (palladium catalyst can be deactivated by sulfur-containing compounds (some media contain cysteine, which can deactivate catalyst), water, or age; reactivate by heating at 160 degrees C for 2 hours in a dry heat oven, or replace with fresh catalyst). The jar should not be over-pressurized (gas packs generate gas - do not use more than the recommended number of gas packs for the jar size; over-pressurization can cause jar failure or leaks). For polycarbonate jars, verify autoclave rating (some polycarbonate jars are autoclavable at 121 degrees C, but repeated autoclaving can cause cloudiness and brittleness over time - check manufacturer instructions; some jars should be disinfected rather than autoclaved). The jar should be handled carefully (dropping can crack polycarbonate or deform stainless steel, compromising the seal). For long-term anaerobic culture work, consider an anaerobic chamber (glove box) for higher throughput and better control, but the anaerobic jar is sufficient for low-to-medium volume and is more economical and space-efficient. Waste (used gas packs, contaminated plates, cultures) should be disposed of per biohazard regulations (autoclave contaminated materials before disposal).
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