Introduction
A microplate reader, also known as a plate reader, is a laboratory instrument used to measure the absorbance, fluorescence, or luminescence of samples in microplates, such as 96-well, 384-well, and other plate formats, and it is used in molecular biology, biochemistry, clinical diagnostics, drug discovery, and many other fields for microplate-based assays. The reader consists of an optical system with a light source, such as a lamp or LED, for absorbance and fluorescence measurements, a monochromator or filters for wavelength selection, a detector, such as a photodiode or photomultiplier, and a plate holder that positions the microplate; in operation, the microplate with the samples and reagents is placed in the reader, and the instrument measures the absorbance, fluorescence, or luminescence of each well, providing quantitative data for the assays, and it can measure in various modes, including endpoint, kinetic, and spectral scanning, and it is used with data analysis software. Microplate readers are used for enzyme-linked immunosorbent assays (ELISA), for protein and nucleic acid quantification, for enzyme activity assays, for cell viability assays, for reporter gene assays, for drug screening, for colorimetric and fluorometric analysis, for clinical diagnostics, for food and environmental testing, and for many other applications, and they are available in various configurations, from single-mode readers to multi-mode systems, with features such as temperature control, shaking, and multiple measurement modes. With their versatility and high throughput, microplate readers are essential instruments for microplate-based assays.
Microplate Reader LMPR-T2010
Microplate Reader supports a wavelength range of 340 to 750 nm for versatile absorbance measurements. Equipped with a vibration plate function, both the time and speed of shaking can be...
Microplate Reader LMPR-T2010
Microplate Reader has a wavelength range of 400 to 750 nm for accurate processing of biological samples. It is designed with a 96-well plate for high-throughput analysis. Our unit employ...
Microplate Reader LMPR-T2021
Microplate Reader a high-resolution 10.4" color intelligent software simplifies test setup and results analysis. Our reader is ideal for diagnostic and pharma labs, enabling nucleic acid...
Microplate Reader LMPR-T2017
Microplate Reader 96/48-well microplates, offering flexible testing options. The photodetector uses a high-precision silicon photodiode. Its light source includes an 8V/50W controlled tu...
Microplate Reader LMPR-T2016
Microplate Reader within a wavelength range of 400nm to 800nm for optimal performance. The device can perform 12 different tests simultaneously on a single plate. It has auto lamp adjust...
Microplate Reader LMPR-T2011
Microplate Reader is compatible with 96-well plates and offers a readout range from 0.000 to 3.500 Absorbance. It features 3 shaking modes with adjustable timing for flexible sample prep...
Microplate Reader LMPR-T2030
Microplate reader is cost effective, efficient and reliable microplate reader. It is well equipped with comprehensive qualitative and quantitative data evaluation function, optical syste...
Microplate Reader LMPR-T2020
Microplate Reader offers a broad wavelength range of 400 to 850 nm and a filter wheel for expanded application flexibility. It provides an absorbance range from 0.000 to 4.000 Abs, with ...
Multi-Mode Microplate Reader LMMR-T2010
Multi-Mode Microplate Reader supports 6 to 384 well plates, including 96-well. It uses a xenon lamp, offers temperature control from RT+4 °C to 45 °C (±0.5 °C at 37 °C), and ensures...
Microplate Reader LMPR-T2010
Microplate Reader features spectrum freedom with a wavelength range from 200 to 1000 nm in precise 1 nm steps. It helps achieve rapid, high-precision readings, completing a full 96-well ...
Microplate Reader LMPR-T2015
Microplate Reader offers exceptional accuracy (±0.008A) and stability (±0.003A) across a broad wavelength range. It has high-speed reading (≤3s) and a built-in thermal printer to get ...
Microplate Reader LMPR-T2013
Microplate Reader reads both 48 well and 96 well microplates in the wavelength range of 400 nm to 850 nm and also it can perform 12 different test in one plate. Features 8 channel optica...
Microplate Reader LMPR-T2014
Microplate Reader is a bench top unit, used in ultraviolet-visible spectrophotometer measurement of 96 well microplate in 10 seconds and 384 well microplate in 30 seconds, it offers line...
Microplate Reader LMPR-T2012
Microplate Reader reads 96 well microplate in the wavelength range of 400 nm to 850 nm and also it can perform 12 different test in one plate. Features 8 channel optical fiber scanning, ...
Standards
IEC 61010-2-101:2018-Particular requirements for in vitro diagnostic (IVD) medical equipment
IEC 61010-2-081:2015-Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes
Complies with the above standards, but is not limited to them.
Features
1. Microplate reader for measuring absorbance, fluorescence and luminescence in microplates.
2. Multi-mode detection for high-throughput screening and quantitative analysis in 96/384-well plates.
3. Suitable for pharmaceutical, biotechnology, clinical, academic, industrial and research labs.
4. Includes optical system, plate handler, detector, controller, software and operation manual.
5. Supports high-throughput absorbance, fluorescence and luminescence assays.
FAQ
Q1: What testing scenarios is this reader mainly applied to?
A: The microplate reader is used in pharmaceutical research, biotechnology, clinical diagnostics, academic research, industrial quality control, food testing and environmental laboratories to measure absorbance, fluorescence and/or luminescence in microplates (96-well, 384-well, 1536-well) for high-throughput screening, quantitative analysis and diagnostic assays. It supports pharmaceutical drug screening (high-throughput screening of compound libraries, IC50/EC50 determination), molecular biology assays (ELISA, nucleic acid quantification, protein quantification), cell-based assays (cell viability, proliferation, cytotoxicity, apoptosis, reporter gene assays), clinical diagnostics (ELISA-based tests, immunoassays), enzyme activity assays (kinase, protease, dehydrogenase), food safety testing (mycotoxins, allergens, pathogens by ELISA), environmental testing (toxins, contaminants), and academic research assays. The microplate reader provides rapid, multi-sample measurement (96 or more samples simultaneously) with high sensitivity and accuracy, essential for high-throughput research and diagnostic applications.
Q2: What standards does the reader comply with?
A: The microplate reader follows relevant ISO, ASTM, CLSI and GB/T standards for laboratory photometric/fluorometric instruments, including IEC 61010 laboratory electrical safety standards, IEC 61326 EMC standards, and applicable in vitro diagnostic (IVD) regulations if used for clinical diagnostics (FDA, CE, NMPA). The hardware complies with applicable safety standards. For absorbance, follow relevant photometric accuracy standards (NIST-traceable calibration filters). For fluorescence/luminescence, use certified reference standards (fluorescein, rhodamine, luminescence standards). For clinical use, follow ISO 15189, CAP or CLIA as applicable, and the reader should be IVD-registered if used for diagnostic purposes. Software should comply with IEC 62304 (medical device software) and 21 CFR Part 11 for regulated use. Users should verify regulatory approval status for clinical applications.
Q3: What is the warranty period for the whole reader and key components?
A: The whole microplate reader is covered by a 12-month warranty. The optical system (lamps, filters, monochromator, detectors), plate handler, controller, software and housing are covered, while lamps (xenon, tungsten, deuterium), filters and fuses are consumables or accessories. Damage from improper use, lamp failure, optical contamination or unauthorized repair is excluded. Extended service contracts are standard for this analytical instrument (typically includes preventive maintenance, lamp replacement, calibration and software support).
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained laboratory personnel familiar with microplate assays, detection modes and data analysis. We provide comprehensive training, operation manuals and application support. Proper assay setup (plate type, reagent volumes, incubation), detection mode selection (absorbance, fluorescence, luminescence), wavelength/filter selection, calibration, quality control, data analysis (curve fitting, concentration calculation) and regulatory documentation are important for accurate, reliable assay results.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Absorbance calibration with NIST-traceable filters recommended every 6-12 months. Fluorescence calibration with certified reference standards (fluorescein, rhodamine) per schedule. Luminescence verification with certified light source or reference. Wavelength accuracy verification periodically. Lamp intensity monitoring (replace when output degrades). Routine quality control per batch for clinical use. Full system performance verification annually. Software updates and data integrity checks per regulatory requirements. Plate handler alignment verified.
Q7: Are supporting consumables universal?
A: Lamps (xenon flash, tungsten, deuterium), filters (if filter-based), microplates (96-well, 384-well - specific plate type may be required for optimal performance), calibration standards, cleaning supplies and fuses are available from laboratory suppliers, though lamp/filter type should match the reader. Long-term consumable cost depends on usage and lamp replacement frequency (xenon flash lamps last longer than continuous lamps).
Q8: Can I customize parameters or supporting accessories?
A: Customization includes detection modes (absorbance, fluorescence top/bottom, luminescence, time-resolved fluorescence, FRET, FP, AlphaScreen), wavelength selection (filters, monochromator, both), plate types (96, 384, 1536 well), temperature control, shaking, plate stacker/automation integration, injector system (for reagent addition), software features (data analysis, curve fitting, LIS/LIMS connectivity, 21 CFR Part 11), connectivity and accessories. Additional filters, injectors and accessories can be ordered separately. Custom orders extend delivery lead-time significantly.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, microplate readers include on-site installation, commissioning, calibration, software setup, validation support (IQ/OQ for regulated use) and operator training by certified specialists. Site preparation guidance is provided before delivery, including environmental requirements (temperature, humidity, dust, vibration).
Q10: What types of assays are compatible with this equipment?
A: The microplate reader measures absorbance (colorimetric assays: ELISA, MTT, protein assays like BCA/Bradford, nucleic acid quantification at 260/280 nm, enzyme activity), fluorescence (fluorometric assays: GFP/reporter assays, calcium assays, ROS detection, DNA quantification with dyes like PicoGreen, HTS with fluorescent probes), and luminescence (luciferase reporter assays, cell viability (CellTiter-Glo), cytotoxicity (LDH), BRET assays, chemiluminescent ELISA) in 96-well, 384-well or 1536-well microplates within the assay type, detection mode, wavelength and plate specification. Different assays require specific detection modes and wavelengths: ELISA (absorbance at 450 nm), MTT (absorbance at 570 nm), GFP (fluorescence ex 488/em 510), luciferase (luminescence, no filter needed). Users should select appropriate detection mode for the assay (absorbance for colorimetric, fluorescence for fluorometric, luminescence for light-emitting), use appropriate microplates (clear-bottom for absorbance, black/white for fluorescence/luminescence - black for fluorescence reduces cross-talk, white for luminescence maximizes signal), set correct wavelengths/filters (ex/em for fluorescence, single wavelength for absorbance), include proper controls (blank, negative, positive, standard curve), calibrate per schedule, run quality control per batch, follow proper assay protocol (incubation times, temperatures, reagent additions - use injector if timing is critical), analyze data with appropriate curve fitting (4-parameter logistic for ELISA, linear for standard curves), report results with assay method, detection mode and units specified, and for clinical/diagnostic use, follow validated assays and regulatory requirements (CLIA, CAP, ISO 15189). The reader should be placed in a temperature-controlled, low-vibration, dust-free environment (temperature fluctuations affect assay results, especially for cell-based assays; dust can contaminate optics). The optical system should be kept clean (dust on filters/monochromator reduces sensitivity - clean per manufacturer instructions). The plate handler should be maintained per schedule (alignment, lubrication). For fluorescence assays, minimize photobleaching (reduce exposure time, use lower lamp intensity if possible). For luminescence assays, ensure dark adaptation (no ambient light - close reader lid, avoid opening during measurement). For cell-based assays, use temperature control (37 degrees C) and CO2 control if available (or work quickly to maintain cell viability). The reader should be calibrated periodically with certified standards. For high-throughput screening, use plate stackers or automation integration for increased throughput. The software should be properly validated for regulated use (21 CFR Part 11 compliance: audit trails, electronic signatures, access control). Waste (used microplates, reagents) should be disposed of per biohazard/chemical waste regulations. The reader should not be moved frequently (can affect optical alignment - if moved, recalibrate). For assays requiring reagent injection (kinetic assays, flash luminescence), use the injector system with proper priming and calibration. The microplate should be properly seated in the reader (no edge effects - use proper plate type, avoid bubbles in wells). For quantitative assays, include a standard curve on each plate (intra-plate variation) and run replicates (inter-plate variation).
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