Introduction
Microplate Reader features spectrum freedom with a wavelength range from 200 to 1000 nm in precise 1 nm steps. It helps achieve rapid, high-precision readings, completing a full 96-well plate in seconds. The reader offers a unique dual-detector system that ensures exceptional stability and accuracy for results you can trust. This microplate reader is built with a long-life xenon flash lamp and an automated self-calibration system. Our is ideal for nucleic acid/protein quantification, , enzyme activity & kinetic studies, cell proliferation & cytotoxicity assays, and microbial growth.
This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.Applications
Our Microplate Reader is ideally suited for quantifying nucleic acids and proteins, performing assays, and measuring enzyme activity. It is also extensively used in cell-based applications, such as cytotoxicity, proliferation, and apoptosis studies. Furthermore, it supports microbial identification and bacterial concentration analysis.
Specifications
| Light Source | Xenon flash lamp (10⁹ flashes lifespan) |
| Wavelength Range | 200 to 1000 nm |
| Wavelength Selection | Grating monochromator |
| Wavelength Increment | 1 nm |
| Wavelength Accuracy | ± 2 nm |
| Wavelength Repeatability | 0.2 nm |
| Bandwidth | < 2.5 nm |
| Detection System | Dual silicon photodiodes (measurement & reference) |
| Absorbance Range | 0 to 4.0 OD |
| Linearity (@450 nm) | R² > 0.999 (0.0 to 3.0 Abs) |
| Absorbance Accuracy (@450 nm) | ±(1.0% + 0.003 Abs) (0 to 2.0 Abs); ±2.0% (2.0 to 2.5 Abs) |
| Absorbance Repeatability (CV @450 nm) | ≤ 0.5% (Accurate mode); ≤ 1.0% (Fast mode) |
| Detection Modes | Endpoint, Kinetic, Spectral Scan, Well Scan |
| Read Time (96-well plate) | Fast Mode: 6 s; Accurate Mode: < 25 s |
| Temperature Range | RT +4 °C to 45 °C |
| Temperature Accuracy (@37°C) | ± 0.5 °C (well-to-well) |
| Temperature Uniformity | < 1.0 °C (96-well plate, without cover) |
| Type | Linear shaking |
| Speeds | 3 settings: Low (5 Hz), Medium (11 Hz), High (20 Hz) |
| Background Shaking | Supported during kinetic assays |
| User Interface | 10-inch capacitive touch screen (Android OS) |
| Operation | Independent use via built-in software or PC software |
| Robotic Arm | Temporarily incompatible |
| Cuvette Mode | Dedicated port with incubation & software |
| Data Export | One-click export to Excel, detailed report generation |
| Internal Storage | 16 GB (stores >20,000 data files) |
| Data Transfer | protocol for direct server upload |
| Ports | 1 x Type B, 2 x Type A |
| Network Port | 1 x |
| Power Supply | DC 24 V, 6.67 A |
| Dimensions | 300 x 500 x 250 mm |
| Net Weight | 15.5 kg |
Features
Built-in Software
Dedicated cuvette port
Dual silicon photodiodes
Long-life xenon flash lamp
Android-based operating system
Automatic self-test and calibration
Quantitative analysis & curve fitting
High-precision grating monochromator
Full-spectrum scanning in 1 nm increments
UV/Vis spectrophotometer with Cuvette port
FAQs
1. What is a Microplate Reader?
A Microplate Reader, commonly called as plate reader or plate spectrophotometer, is an analytical instrument that reads or detects the physical, chemical, and biological events of the analyte, within the wells of the plate. It has numerous tiny wells where the sample analyte is introduced for analysis.
2. What is the difference between a spectrophotometer and a microplate reader?
A standard spectrophotometer measures the absorbance of one sample at a time. The sample is typically placed in a cuvette through which light is sent horizontally. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well vertically.
3. What is the working principle of a microplate reader?
Microplate-based measurements detect light signals produced by a sample, converted by a sample, or transmitted through a sample. In the plate reader, the signal is measured by a detector, usually a photomultiplier tube (). PMTs convert photons into electricity that is then quantified by the microplate reader. The output of this process is numbers by which a sample is quantified.
4. What is the light source typically used in plate readers?
Tungsten-halogen or xenon flash lamps are generally used. LEDs have also come into the picture in some modern readers.
5. What is the detection technology of a plate reader?
The detection technology can be of 3 types- Monochromator based detection which provides flexibility, convenience, and spectral scanning; Filter based detection which enables rapid switch between different wavelengths; Hybrid detection which has dual technological advantage of both flexibility and sensitivity.
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