Multi-Mode Microplate Reader LMMR-T2010

Introduction

Multi-Mode Microplate Reader supports 6 to 384 well plates, including 96-well. It uses a xenon lamp, offers temperature control from RT+4 °C to 45 °C (±0.5 °C at 37 °C), and ensures high sensitivity with a wide dynamic range. It can perform absorbance, fluorescence, and luminescence detection, with advanced options like fluorescence polarization and time-resolved fluorescence. Our multi-mode microplate reader is designed for medical, biological, and pharmaceutical research, it provides accurate analysis for molecular biology, biochemistry, and cytology.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Multi-Mode Microplate Reader is widely used in molecular biology, biochemistry, cell biology, drug discovery, and clinical research.

Specifications

Microplate Types	Supports 6 to 384 well plates; 96-well included
Light source	Xenon lamp
Temperature Control	RT +4 °C to 45 °C ± 0.5 °C at 37 °C
Dynamic Range	0 to 4 OD range supported
Shaking: Linear and orbital shaking	Linear, annular, double annular shaking modes available
Reading Speed	<15 s for 96-well plate fast mode
Precision	CV <1.0 % or SD <0.003
Accuracy	± (1.0% + 0.003Abs) at 0 to 2.0 Abs
Ratio accuracy	Data precision supported
Wavelength selection	Advanced monochromator system 200 to 1000 nm
Fully controlled by software (open/close)	Supported via ReaderIt-II software Optional automatic injector blocks available Compatible with Win7/Win10, external PC supported
Absorbance Detector	UV-silicon photodiode
Absorbance Wavelength	200 to 1000 nm
Absorbance Wavelength accuracy	±0.5 nm >315 nm, ±0.3 nm ≤315 nm
Wavelength reproducibility (Absorbance)	±0.5 nm >315 nm, ±0.3 nm ≤315 nm
low volume	Low-volume detection supported
Fluorescence Detector	detector
Excitation and Emission wavelength	EX 200 to 1000 nm; EM 270 to 850 nm
Fluorescence bottom reading sensitivity	Detection limit as low as 5 amol/well
Fluorescence intensity bottom Mono	Supports low
Fluorescence polarization sensitivity	FP detection sensitivity 5 mP
Excitation bandwidth	<5 nm ≤315 nm; <9 nm >315 nm
Emission bandwidth	<20 nm
Fluorescence Wavelength accuracy	±2.0 nm >315 nm; ±1.0 nm ≤315 nm
Fluorescence Wavelength reproducibility	±1.0 nm >315 nm; ±0.5 nm ≤315 nm
Dimension	420 × 550 × 386 mm
Packaging Dimension	500 × 600 × 450 mm
Net Weight	33 Kg
Gross Weight	40 Kg

Features

Rotating Touch Screen

Modular Filter

Flexible Software

Xenon lamp

FAQs

1. What type of light source does the system use, and how reliable is it?

The reader uses a UV Xenon flashlamp as its light source. This type of lamp ensures a stable, reliable, and consistent energy output across the full wavelength range of 200–1000 nm, supporting accurate and repeatable measurements in absorbance, fluorescence, and luminescence assays.

2. Does the offer temperature control, and what accuracy does it maintain?

Yes, the instrument offers advanced temperature control, ranging from room temperature +4 °C up to 45 °C. It maintains a very stable environment with an accuracy of ±0.5 °C at 37 °C. This precise temperature regulation is critical for assays that require consistent incubation conditions.

3. What are the optical performance specifications for absorbance mode?

For absorbance measurements, the system uses a UV-silicon photodiode detector and covers a wavelength range of 200–1000 nm. The wavelength accuracy is ±0.5 nm for wavelengths greater than 315 nm and ±0.3 nm for 315 nm or below. Similarly, wavelength reproducibility is ±0.5 nm above 315 nm and ±0.3 nm at or below 315 nm. The reader provides a dynamic range of 0–4 OD, with high precision at 260 nm (CV <1.0% or SD <0.003) and accuracy of ±(1.0% + 0.003 Abs) at 0–2.0 Abs. It also offers ratio accuracy of ±0.07 at 260/280 nm.

4. What are the optical performance specifications for fluorescence mode?

For fluorescence measurements, the reader uses a photomultiplier tube () detector that is UV- and red-sensitive, ensuring detection across a wide spectrum. The excitation wavelength range is 200–1000 nm, and the emission range is 270–850 nm. It achieves high sensitivity with bottom detection as low as 5 amol per well and an intensity detection limit of 45 PM. Fluorescence polarization is supported with sensitivity at 1 nM (SD <4 m), and FP detection is accurate up to 5 mP. Bandwidth control is also provided: excitation bandwidth is <5 nm at ?315 nm and <9 nm above 315 nm, while emission bandwidth is <20 nm. Wavelength accuracy is ±2.0 nm for >315 nm and ±1.0 nm for ?315 nm, with reproducibility of ±1.0 nm and ±0.5 nm respectively.

5. What shaking and mixing options are available in the reader?

The provides multiple shaking modes to suit different assay requirements. It supports linear shaking, annular shaking, and double annular shaking modes. These features ensure proper sample mixing, uniform reaction conditions, and reliable assay outcomes.

6. Does the reader support low-volume sample analysis, especially for detection?

Yes, the system is optimized for sensitive detection in low-volume samples. It supports detection down to 1 ng/µl in low-volume conditions, which makes it suitable for applications in molecular biology and genomics where sample amounts are often limited.

7. How is the system controlled, and does it meet regulatory compliance standards?

The instrument is fully controlled by software, allowing automated operation, including opening and closing of the system. It comes with ReaderIt-II software, which is 21 Part 11 compliant. This ensures secure data handling, audit trails, and compliance with regulatory requirements for laboratories working under / standards. Additionally, it is compatible with external PCs (Win7/Win10 with Core i5 or higher) and printers for flexible data management.

8. What additional features and support are available with the system?

The includes optional compatibility with automated liquid handling through injector blocks, allowing future scalability. Installation, qualification, and onsite training are typically provided, even though not explicitly listed in the catalog, ensuring smooth adoption in the lab. The system also provides broad connectivity and expandability options, making it a future-ready solution for modern laboratory workflows.

9. Does the Multi-Mode Microplate Reader support multiple assay modes, and how does it handle wavelength selection?

Yes, the supports absorbance, fluorescence, and luminescence assays, making it highly versatile for different experimental needs. It uses an advanced monochromator system for wavelength selection, which allows coverage from 200 to 1000 nm. This system provides two excitation and two emission options, ensuring precise wavelength selection for different assays.

10. What types of microplates are compatible with the reader, and how fast can it process them?

The is designed to support a wide variety of microplates ranging from 6 wells up to 384 wells, including standard 96-well plates. It is also capable of reading a full 96-well plate in less than 15 seconds, which makes it efficient for high-throughput applications.

11. What is a Microplate Reader?

A Microplate Reader, commonly called as plate reader or plate spectrophotometer, is an analytical instrument that reads or detects the physical, chemical, and biological events of the analyte, within the wells of the plate. It has numerous tiny wells where the sample analyte is introduced for analysis.

12. What is the difference between a spectrophotometer and a microplate reader?

A standard spectrophotometer measures the absorbance of one sample at a time. The sample is typically placed in a cuvette through which light is sent horizontally. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well vertically.

13. What is the working principle of a microplate reader?

Microplate-based measurements detect light signals produced by a sample, converted by a sample, or transmitted through a sample. In the plate reader, the signal is measured by a detector, usually a photomultiplier tube (). PMTs convert photons into electricity that is then quantified by the microplate reader. The output of this process is numbers by which a sample is quantified.

14. What is the light source typically used in plate readers?

Tungsten-halogen or xenon flash lamps are generally used. LEDs have also come into the picture in some modern readers.

15. What is the detection technology of a plate reader?

The detection technology can be of 3 types- Monochromator based detection which provides flexibility, convenience, and spectral scanning; Filter based detection which enables rapid switch between different wavelengths; Hybrid detection which has dual technological advantage of both flexibility and sensitivity.