Introduction
Microplate Reader is a bench top unit, used in ultraviolet-visible spectrophotometer measurement of 96 well microplate in 10 seconds and 384 well microplate in 30 seconds, it offers linear plate shaking motion. Designed with flashing xenon lamp source, long life and stable illumination, the wavelength ranges from 200 nm to 1000 nm and offers 0 to 4.0 OD measurement range. It offers free wavelength selection, so being an ideal tool for virtually any photometric research application, such as , , protein analysis and more it offers exceptional usability through its intuitive, powerful PC Reader It-II Software. It has been designed to deliver high performance and high quality results. The special light absorption detection board can be used for calibration of the equipment also used in powerful data analysis software, microplate analysis and workstation.
This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.Applications
Our Microplate Reader is essential for life science research, including / analysis, cell proliferation and cytotoxicity tests, bacterial concentration analysis, and pharmaceutical plant studies. It is also used for protease, kinase, phospholipase, , and activity tests, as well as end-point method /, offering a variety of fitting curves for comprehensive analysis.
Specifications
| Wavelength range | 200 nm to 1000 nm with 1 nm steps |
| Wavelength accuracy | ±2 nm |
| Wavelength selection | Monochromator |
| Half-bandwidth of filter | ˂ 2.5 nm |
| Repetition | ±0.2 nm |
| Measurement range | 0 to 4.0 OD |
| Detector | Two silicon photoelectric detector, one for measurement, another for reference |
| Linearity at 450 nm | 0 to 2.5 Abs, ±2% (96 well plates) |
| Accuracy at 450 nm | 1.0 % + 0.005 Abs (0 - 2.0 Abs), 2.0 % [2.0 to 2.5 Abs) |
| Precision at 450 nm | CV < 0.5 % accurate model, CV < 1.0 % fast mode |
| Test speed 01 | 10 seconds with 96-well plate (fast mode), 30 seconds with 96-well plate (accurate model) |
| Plate shaking | Linear, three options for speed |
| Test speed 02 | 15 seconds with 96-well plate (single wavelength), 30 seconds with 96-well plate (double wavelength) |
| Ambient Temperature | +5℃ to 45℃ |
| Software | Integrated software or PC control software |
| Light source | Xenon flash lamp |
| Display | 10 inch touch screen |
| Analysis software | Reader It- II |
| User interface | Touch screen, android system, 10 inch touch screen, external keyboard mouse |
| Storage | 16 G memory, more than 10,000 test records can be stored |
| Ports | 3 ports, for PC, printer and -disk |
| Automated systems | Temporarily unable compatible with automated systems |
| Power Consumption | DC 24 V 6.5 A |
| Dimension | 300 × 500 × 290 mm |
| Packaging Dimension | 800 × 700 × 800 mm |
| Net Weight | 15.5 kg |
| Gross Weight | 36 Kg |
Features
Temperature control incubation system
Microplate UV-visible spectrophotometer
real-time spectral scanning
96 and 384 well microplates
10-inch touchscreen, stand-alone operation
Wavelength range: 200-1000 nm
Microplate oscillating mixing function
Cell proliferation, cytotoxicity test
Flashing xenon lamp source
data interface, instrument control
Absorbance, concentration, nucleic acids, proteins
Automated parameter setting, self-test
Reader It-II PC software, data validation
and Wi-Fi data access
FAQs
1. What capacity of well plate can be used with Microplate Reader ?
product is designed to accept both 96 and 384 well plates, which can be used in high throughput applications.
2. Are there any associated software with Microplate Reader ?
Microplate Reader comes with an integrated, user-friendly Reader It- II software to ease the process of data analysis. The data can be exported in .csv or .exe file formats.
3. What is a Microplate Reader?
A Microplate Reader, commonly called as plate reader or plate spectrophotometer, is an analytical instrument that reads or detects the physical, chemical, and biological events of the analyte, within the wells of the plate. It has numerous tiny wells where the sample analyte is introduced for analysis.
4. What is the difference between a spectrophotometer and a microplate reader?
A standard spectrophotometer measures the absorbance of one sample at a time. The sample is typically placed in a cuvette through which light is sent horizontally. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well vertically.
5. What is the working principle of a microplate reader?
Microplate-based measurements detect light signals produced by a sample, converted by a sample, or transmitted through a sample. In the plate reader, the signal is measured by a detector, usually a photomultiplier tube (). PMTs convert photons into electricity that is then quantified by the microplate reader. The output of this process is numbers by which a sample is quantified.
6. What is the light source typically used in plate readers?
Tungsten-halogen or xenon flash lamps are generally used. LEDs have also come into the picture in some modern readers.
7. What is the detection technology of a plate reader?
The detection technology can be of 3 types- Monochromator based detection which provides flexibility, convenience, and spectral scanning; Filter based detection which enables rapid switch between different wavelengths; Hybrid detection which has dual technological advantage of both flexibility and sensitivity.
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