Introduction
Microplate reader is cost effective, efficient and reliable microplate reader. It is well equipped with comprehensive qualitative and quantitative data evaluation function, optical system with 8 channel optical fiber, auto plate centering system and stable memory.
This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.Applications
It finds wide application in Immunoassays (), enzyme assays, absorbance based microplate reader assay (Bradford assay), proliferation assays and toxicity.
Specifications
| Plate type | 48/96-well plate |
| Wavelength range | 400-850 nm |
| Standard filters | 405, 450, 492, 630 nm |
| Additional filters | Filter wheel for up to 8 filters |
| Reading range | 0.000 to 4.000 Abs |
| Reading speed | Single wavelength ≤ 3s; dual wavelength ≤ 6s |
| Resolution | 0.001 Abs |
| Accuracy | ± 0.008 Abs |
| Reproducibility | ≤ 0.2 % |
| Stability | ± 0.003 Abs |
| Shaking mode | Linear shaking, 3 speeds |
| Photodetector | Silicon photodiode |
| Light source | 8V/50W controlled tungsten halogen lamp |
| Interface connection | |
| Power supply | 100 to 240 V, 50 to 60 Hz |
| Dimension (LxWxH) | 460 × 350 ×197 mm |
| Net weight | 10 kg |
| Gross weight | 12 kg |
Features
Measures single or dual wavelength
Wide wavelength range of 400 to 850 nm
Reads both 48 and 96-well plate
Needs to be connected with computer to operate
Capable of performing 12 different tests in just one plate
Optical system with 8 channel optical fiber scanning
Auto plate centering system to position the center of the cell accurately
Auto-lamp adjustment and auto calibration function
Stable memory capable of storing 10,000,000 test data and 500 test results
Multiple formats of result output
port for easy data transfer
Consists Reader software
FAQs
1. How 48/96 well Microplate Reader does is beneficial over other high numbered well plate readers?
48 to 96 well plates can accommodate more sample volume and detect with great sensitivity in very less amount of time, thereby results in quick and easy analysis, as compared to other high numbered well plates such as 1536 to 3456 readers.
2. What is the advantage of a single mode operation?
Microplate Reader can be used for studies having one particular application which demands high repeatability and sensitivity. It is suitable for monitoring of microbial growth which takes more than one day, and thioflavin T experiments which takes up to 7 days.
3. What is a Microplate Reader?
A Microplate Reader, commonly called as plate reader or plate spectrophotometer, is an analytical instrument that reads or detects the physical, chemical, and biological events of the analyte, within the wells of the plate. It has numerous tiny wells where the sample analyte is introduced for analysis.
4. What is the difference between a spectrophotometer and a microplate reader?
A standard spectrophotometer measures the absorbance of one sample at a time. The sample is typically placed in a cuvette through which light is sent horizontally. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well vertically.
5. What is the working principle of a microplate reader?
Microplate-based measurements detect light signals produced by a sample, converted by a sample, or transmitted through a sample. In the plate reader, the signal is measured by a detector, usually a photomultiplier tube (). PMTs convert photons into electricity that is then quantified by the microplate reader. The output of this process is numbers by which a sample is quantified.
6. What is the light source typically used in plate readers?
Tungsten-halogen or xenon flash lamps are generally used. LEDs have also come into the picture in some modern readers.
7. What is the detection technology of a plate reader?
The detection technology can be of 3 types- Monochromator based detection which provides flexibility, convenience, and spectral scanning; Filter based detection which enables rapid switch between different wavelengths; Hybrid detection which has dual technological advantage of both flexibility and sensitivity.
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