Introduction
A double beam UV-Vis spectrophotometer is an analytical instrument that measures the absorption of ultraviolet and visible light by a sample as a function of wavelength, using a dual-beam optical design that simultaneously compares the light transmitted through the sample with a reference beam, providing high accuracy, stability, and reproducibility for quantitative and qualitative analysis. In operation, light from a deuterium or tungsten-halogen source passes through a monochromator, which selects a narrow band of wavelengths, and is then split by a beam splitter or rotating mirror into two beams; one beam passes through the sample cell and the other through a reference cell containing the solvent or blank, and the instrument measures the intensity of the two beams alternately or simultaneously, calculating the absorbance of the sample according to the Beer-Lambert law, which relates absorbance to the concentration of the absorbing species and the path length; the dual-beam configuration compensates for fluctuations in the light source, drift in the electronics, and variations in the optical path, so that measurements remain stable and accurate over time. Double beam UV-Vis spectrophotometers can scan a wavelength range, typically from about 190 to 1100 nm, covering the ultraviolet and visible regions, and they can produce absorption spectra that provide information about the identity, concentration, and purity of compounds. They are used in chemistry, biochemistry, molecular biology, pharmacy, environmental science, food science, and materials science for quantifying analytes, for determining protein and nucleic acid concentrations, for measuring color and turbidity, for verifying the purity of products, for studying reaction kinetics, and for method development. Features include wavelength scanning, high spectral resolution, automatic measurement, and data analysis software. With their accuracy, stability, and versatility, double beam UV-Vis spectrophotometers are essential analytical instruments in research and quality control laboratories.
Double Beam UV-Vis Spectrophotometer LUS-T2044
Double Beam UV-Vis Spectrophotometer offers fast performance, completing a full spectrum scan in 2 seconds at 30,000 nm/min. It operates with a minimum spectral bandwidth of 0.2 nm, ens...
Double Beam UV/Vis Spectrophotometer LUS-T2070
Double Beam UV/Vis Spectrophotometer offers a Czerny-Turner (CT) design with a 1200-line grating for superior accuracy and stability. It features ±0.3nm wavelength accuracy and ±0.3%T ...
Double Beam UV-Vis Spectrophotometer LUS-T2064
Double Beam UV-Vis Spectrophotometer offers a wavelength range of 320 to 1100 nm for stable measurements across the UV-Vis spectrum. It provides high photometric accuracy for reliable de...
Double Beam UV-Vis Spectrophotometer LUS-T2063
Double Beam UV-Vis Spectrophotometer features a spectral wavelength of 2.0nm, ensuring reliable absorbance measurements across UV and visible ranges. It has dual beam optics for high acc...
Double Beam UV-Vis Spectrophotometer LUS-T2039
Double Beam UV-Vis spectrophotometer offers 190 to 1100 nm and a spectral bandwidth of 2 nm. It has a 10.1-inch high-resolution -colored touch screen and for better user experience. This...
Double Beam UV-Vis Spectrophotometer LUS-T2029
Double beam UV-Vis spectrophotometer offers a wavelength range of 190 to 1100 nm. It offers a versatile spectral bandwidth range of 0.5, 1, 2, 4, and 5 nm, which enables seamless applica...
Double Beam UV-Vis Spectrophotometer LUS-T2028
Double beam UV-Vis spectrophotometer offers a wavelength range of 190 to 1100 nm and a spectral bandwidth of 1 nm. Its in-built memory, saving up to 1024 MB for test data and working cu...
Double Beam UV-Vis Spectrophotometer LUS-T2027
Double Beam UV-Vis Spectrophotometer offers a wavelength range of 190 to 1100 nm and a spectral bandwidth of 2 nm. It is equipped with advanced a 7-inch color screen and a Windows graphi...
Double Beam UV-Vis Spectrophotometer LUS-T2053
Double Beam UV-Vis Spectrophotometer offers a wavelength range of 190 to 1100 nm. It has a versatile spectral bandwidth range of 0.5, 1, 2, 4, and 5 nm, which enables seamless applicatio...
Double Beam UV-Vis Spectrophotometer LUS-T2022
Double Beam UV-Vis Spectrophotometer features a wavelength range of 190 to 1100 nm and a spectral bandwidth of 1 nm. It offers various scanning modes, high, medium, and low, with a maxim...
Double Beam UV-Vis Spectrophotometer LUS-T2021
Double Beam UV-Vis Spectrophotometer offers a wavelength range of 190 to 1100 nm with a spectral bandwidth of 2nm. It offers automatic wavelength settings ensures accurate and consistent...
Double Beam UV-Vis Spectrophotometer LUS-T2019
Double Beam UV-Vis Spectrophotometer offers a wavelength range of 190 to 1100 nm. It has a versatile spectral bandwidth range of 0.5, 1, 2, 4 and 5 nm, that flexibility enables seamless ...
Double Beam UV-Vis Spectrophotometer LUS-T2018
Double Beam UV-Vis Spectrophotometer offers a wavelength range of 190 to 1100 nm and a spectral bandwidth of 2 nm. It supports various scanning modes including high, med, and low with a ...
Double Beam UV-Vis Spectrophotometer LUS-T2014
Double Beam UV-Vis Spectrophotometer designed with a wavelength range of 190 to 1100 nm and a spectral bandwidth of 2 nm. It features automatic wavelength setting, ensures precise and c...
Double Beam UV-Visible Spectrophotometer LUS-T2037
Double Beam UV-Visible Spectrophotometer features a wavelength range of 190 to 1100 nm with spectral bandwidth of 1 nm. It has a large internal memory capacity of up to 1024M, that store...
Double Beam UV-Visible Spectrophotometer LUS-T2036
Double Beam UV-Visible Spectrophotometer offers a wavelength range of 190 to 1100 nm with spectral bandwidth of 2 nm. It is equipped with advanced in-house massive memory, capable of sav...
Double Beam UV-Visible Spectrophotometer LUS-T2038
Double Beam UV-Visible Spectrophotometer offers a wavelength range of 190 to 1100 nm. It features a flexible spectral bandwidth of 0.5, 1, 2, 4, and 5 nm, allowing for various analytical...
Double Beam UV-Vis Spectrophotometer LUS-T2035
Double beam UV-Vis Spectrophotometer features a wavelength range of 190 to 1100 nm. It supports different work modes including transmittance, absorbance, concentration and energy. The sp...
Double Beam UV-Vis Spectrophotometer LUS-T2034
Double Beam UV-Vis Spectrophotometer offers a wavelength range of 190 to 1100 nm and spectral bandwidths of 2 nm. It supports data storage and a port used for data transfer. The spectro...
Double Beam UV-Visible Spectrophotometer LUS-T2026
Double Beam UV-Visible Spectrophotometer offers a wavelength range of 190 to 1100 nm. It supports spectral bandwidths of 0.1, 0.2, 0.5, 1, 2, 4, and 5 nm. The spectrophotometer enables r...
Double Beam UV-Visible Spectrophotometer LUS-T2025
Double Beam UV-Visible Spectrophotometer features a wavelength range of 190 to 1100 nm. It offers different scanning speeds-high, medium, and low with a maximum speed of 6000 nm/min. Th...
Double Beam UV-Visible Spectrophotometer LUS-T2024
Double Beam UV-Visible Spectrophotometer features a wavelength range of 190 to 1100 nm. It offers adjustable scanning speeds at high, medium, and low settings, reaching a maximum speed o...
Double Beam UV-Vis Spectrophotometer LUS-T2062
Double Beam UV-Vis Spectrophotometer has selectable spectral bandwidths of 0.5 nm, 1 nm, 2 nm, 4 nm, and 5 nm for flexible measurements. Wavelength accuracy of ±0.3 nm across the full r...
Double Beam UV-Vis Spectrophotometer LUS-T2061
Double Beam UV-Vis Spectrophotometer features a fixed 2 nm spectral bandwidth, ensuring consistent optical resolution. It supports an extended photometric range of -0.3 to 4A, enabling a...
Double Beam UV-Vis Spectrophotometer LUS-T2060
Double Beam UV-Vis Spectrophotometer comes with a 0.5 to 5 nm variable bandwidth for flexible analysis. The 190 to 1100 nm wavelength range supports diverse spectral applications. A doub...
Double Beam UV-VIS Spectrophotometer LUS-T2052
Double Beam UV- Spectrophotometer utilizes a double beam optical system with a 1.0 nm bandwidth and wavelength accuracy of ±0.3 nm. Operating over a wide wavelength range of 190 to 1100...
Double Beam UV-Vis Spectrophotometer LUS-T2051
Double Beam UV-Vis Spectrophotometer covers a wavelength range of 190 to 1100 nm with a 5 nm spectral bandwidth. It features a dual silicon photodiode detector for precise light measurem...
Double Beam UV-Vis Spectrophotometer LUS-T2050
Double Beam UV-Vis Spectrophotometer features a spectral bandwidth of 6 nm with a wavelength accuracy of ±2 nm. It is capable of measuring time-based changes and calculating response ra...
Double Beam UV-Visible Spectrophotometer LUS-T2015
UV-Visible Spectrophotometer is a high sensitive double beam spectrophotometer with a wavelength of 190 to 1100 nm for absorbance spectra of chemical and biochemical compounds. Equipped ...
Double Beam UV-Vis Spectrophotometer LUS-T2017
Double beam UV-Vis Spectrophotometer has an optical system with 1.7 nm bandwidth and wavelength range of 190 to 1100 nm. Designed with import source and receiver system for high precisio...
Double Beam UV-Vis Spectrophotometer LUS-T2016
Double beam UV-Vis Spectrophotometer offers a wavelength range from 190 to 1100 nm to accommodate a wide variety of applications. It features high-performance imported light sources and ...
Double Beam UV-Vis Spectrophotometer LUS-T2013
Double Beam UV-Vis Spectrophotometer is a compact, tabletop double beam designed UV-Vis spectrophotometer comprising Silicon photodiode detector, Tungsten and Deuterium lamp as light sou...
Double Beam UV-Vis Spectrophotometer LUS-T2010
Double Beam UV-Vis Spectrophotometer is a compact, tabletop double beam designed UV-Vis spectrophotometer comprising Silicon photodiode detector, Tungsten and Deuterium lamp as light sou...
Double Beam UV-Vis Spectrophotometer LUS-T2033
Double Beam UV-Vis Spectrophotometer covers a wavelength range of 190 to 1100 nm with adjustable spectral bandwidths from 0.1 to 5 nm. It features a double beam optical system with a 120...
Double Beam UV-Vis Spectrophotometer LUS-T2043
Double Beam UV-Vis Spectrophotometer utilizes two separate beams, with a scanning rate of 800 nm/min for simultaneous measurement of the sample and reference. Boasts powerful capabilitie...
Double Beam UV-Vis Spectrophotometer LUS-T2023
Double Beam UV-Vis Spectrophotometer offers a wide wavelength range from 190 to 900nm ensuring compatibility with various fields of study. Fully automatic design simplifies measurement p...
Double Beam UV-Vis Spectrophotometer LUS-T2042
Double Beam UV-Vis Spectrophotometer has an adjustable spectral bandwidth from 0.5nm to 6nm with a variable in 0.1nm increments. Equipped with a dual-beam system that simultaneously meas...
Double Beam UV-VIS Spectrophotometer LUS-T2041
Double Beam UV- Spectrophotometer is a sturdy and long-lasting construction that is enhanced with a graphic display and a color-coded key for easy identification. Configured with highly ...
Double Beam UV-Vis Spectrophotometer LUS-T2012
Double Beam UV-Vis Spectrophotometer is a compact, tabletop unit designed with double beam monochromator, comprising automatic calibration and silicon photocell sensor. Features fixed 2 ...
Double Beam UV-Vis Spectrophotometer LUS-T2020
Double Beam UV-Vis Spectrophotometer is a double beam designed UV-Vis spectrophotometer, offers 190 to 1100 nm of wavelength range with C-T monochromator and 1600 L/mm holographic gratin...
Double Beam UV-Vis Spectrophotometer LUS-T2011
Double Beam UV-Vis Spectrophotometer is a compact, tabletop, wide screen double beam spectrophotometer comprising double beam long light path design, offers 190 to 1100 nm of wavelength ...
Double Beam UV-Vis Spectrophotometer LUS-T2040
Double Beam UV-Vis Spectrophotometer a bench top device with 190 nm to 1100 nm wavelength range, and offers spectral bandwidth of 0.5/1/2/4 nm. Equipped with 16 mm thick rigid aluminium ...
Double Beam UV-Vis Spectrophotometer LUS-T2032
Double Beam UV-Vis Spectrophotometer is bench top device with 190 nm to 1100 nm wavelength range, and offers spectral bandwidth of 0.5/1/2/4/5 nm. With T, A, C, E work mode it is designe...
Double Beam UV Vis Spectrophotometer LUS-T2031
Double Beam UV Vis Spectrophotometer equipped with a high-resolution 10.1-inch touch screen for smooth navigation and control. Its double-beam optical system ensures superior stability a...
Double Beam UV-Vis Spectrophotometer LUS-T2030
Double Beam UV-Visible Spectrophotometer equipped with 190 to1100 nm wavelength range for precise spectral analysis across multiple applications. Its advanced digital display enhances re...
Standards
GB/T 26813-2010-Double-beam ultraviolet-visible spectrophotometers
European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible
ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
USP General Chapter <857>-Ultraviolet-Visible Spectroscopy
Complies with the above standards, but is not limited to them.
Features
1. Double-beam UV-visible spectrophotometer for high-accuracy absorbance measurement.
2. Dual-beam optical system automatically compensates for light source drift.
3. Wide wavelength range with high resolution for spectral scanning and analysis.
4. Suitable for pharmaceutical, chemical, environmental, food and research laboratories.
5. Software supports scanning, quantitation, kinetics and regulatory compliance features.
FAQ
Q1: What testing scenarios is this spectrophotometer mainly applied to?
A: The double-beam UV-visible spectrophotometer is used in pharmaceutical quality control, chemical analysis, environmental monitoring, food safety testing, clinical diagnostics and research laboratories to perform high-accuracy qualitative and quantitative analysis by absorbance measurement in the ultraviolet and visible ranges. It supports drug content and impurity analysis per pharmacopoeial methods, compound identification by spectral scanning, enzyme kinetics, nucleic acid and protein quantitation, water quality testing, food additive analysis and method development. The double-beam design provides superior stability by continuously comparing sample and reference beams, automatically compensating for light source drift during long scans and kinetic measurements. It is the standard for regulated pharmaceutical quality control and research requiring the highest accuracy and reproducibility, particularly for spectral scanning, time-based kinetics and low-absorbance measurements where source instability would affect result quality in pharmaceutical, chemical, environmental and research applications.
Q2: What standards does the spectrophotometer comply with?
A: The instrument supports analytical methods referenced in USP, EP and ChP monographs, ASTM E169 for UV-Vis quantitative analysis, and relevant GB/T standards for spectrophotometry. The hardware complies with IEC 61010 laboratory safety standards. Electronic records may be configured for 21 CFR Part 11 compliance. Wavelength and absorbance accuracy should be verified with certified reference materials.
Q3: What is the warranty period for the whole machine and key components?
A: The whole spectrophotometer is covered by a 12-month warranty. The optical system, detector, light source and controller are covered, while lamps, cuvettes and calibration standards are consumables excluded from warranty. Extended warranty and service contracts are available for this high-value instrument.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained analysts familiar with spectrophotometric principles and method execution. We provide structured training, operation manuals and application support. Routine measurement and spectral scanning can be performed by trained operators with software guidance and standard operating procedures.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Wavelength and absorbance calibration with certified reference materials is recommended every 6-12 months for accredited laboratories. Routine verification before each analytical batch for regulated work. Lamp replacement and optical alignment follow the manufacturer schedule. System suitability testing per pharmacopoeial requirements.
Q7: Are supporting consumables universal?
A: Lamps, cuvettes and calibration standards are available from multiple qualified suppliers, though original lamps and optics are recommended for optimal performance. Long-term consumable cost is moderate depending on usage and light source type.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes wavelength range, resolution, detector type, sample compartment accessories and software modules. Additional accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Standard delivery includes the spectrophotometer and accessories with an operation manual. On-site installation, commissioning and training are not included for standard orders but can be arranged for laboratory setup projects. Remote guidance is provided.
Q10: What types of samples are compatible with this equipment?
A: The instrument analyzes liquid samples within the wavelength and absorbance range. Samples must be clear and homogeneous; turbid samples cause scattering errors. Highly corrosive samples require compatible cuvette material. Very high absorbance samples should be diluted to the linear range. The double-beam design requires proper reference cell placement and baseline correction for optimal performance. Users should allow lamp warm-up, perform system suitability verification, use matched cuvettes, and follow calibration and verification procedures to maintain measurement accuracy and traceability for regulated analytical work.
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