Double Beam UV-Vis Spectrophotometer LUS-T2064

Introduction

Double Beam UV-Vis Spectrophotometer offers a wavelength range of 320 to 1100 nm for stable measurements across the UV-Vis spectrum. It provides high photometric accuracy for reliable detection of even low-concentration analytes in complex samples. The spectrophotometer is equipped with advanced a silicon photodiode detector, ensuring stable and sensitive measurement of light intensity. Our double beam uv-vis spectrophotometer features stable baseline performance for accurate absorbance readings with minimal signal drift.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Double Beam UV-Vis Spectrophotometer splits light into two samples and references to provide real-time correction for fluctuations in light. It plays a key role in chemistry, biochemistry, clinical, environmental, water testing, food and beverage sector.

Specifications

Spectral Bandwidth	2.0nm
Wavelength Range	320 to 1100 nm
Optical System	Quasi double beam ratio monitoring optical path, holographic diffraction grating
Wavelength Accuracy	± 0.5nm
Wavelength Repeatability	0.2nm or less
Wavelength Setting	Automatic Wavelength Setting
Wavelength Resolution	0.1 nm
Luminosity Range	0 to 200% T, -0.3 to 3A, 0 to 9999 C, 0 to 9999 F
Photometric Accuracy	A (0 to 0.5A), 0.02mm ± 0.04 A 0.5 to1 (A), ±0.3%TT (0 to100%)
Photometric Repeatability	≤0.001A (0 to 0.5A), ≤0.002A (0.5 to 1A), T (0 to 100% to 0.15% or less)
Stray Light	≤0.05%T (220/360nm)
Baseline Drift	≤0.001A/h (500nm,0A)
Noise	± 0.1%T (0%T line) ±0.3%T (100%T line) ±0.0005A (500nm)
Baseline Flatness	±0.0015A
Scan Speed	High, medium and low gears are adjustable
Detector	Imported silicon photodiode
Light Source	Imported long-life tungsten and deuterium lamps
Display	6 "320×240 dot matrix display
Keyboard	Digital light touch film keys
Data Interface	Port
Printout	Microprinter, PC (online)
Power Supply	AC 90 to 250V, 50 /60Hz
Overall Dimensions	545×468 ×245mm
Weight	18 kg

Features

Dual Beam Optics

Clear Graphic Display

Calibration Recovery System

Digital Light Touch Film Keys

Wide Measurement Wavelength Range 

FAQs

1. What are common applications of double beam UV-Vis spectrophotometer ?

Double beam UV-Vis spectrophotometer are versatile instruments used across various scientific fields. In biochemistry, they quantify nucleic acids and proteins by measuring absorbance at specific wavelengths. Environmental laboratories use them to detect pollutants in water samples. In pharmaceuticals, they assess drug purity and concentration. The simultaneous measurement of sample and reference enhances accuracy, making them suitable for kinetic studies, quality control, and research applications requiring precise and reproducible data.

2. How does the double beam design improve measurement stability and accuracy in double beam UV-Vis spectrophotometer ?

double beam UV-Vis spectrophotometer configuration compensates for fluctuations in light source intensity and detector sensitivity by simultaneously measuring the sample and reference beams. This real-time comparison allows the instrument to correct for any variations, resulting in more stable and accurate absorbance readings. It minimizes the impact of external factors, such as temperature changes or lamp aging, which can affect single beam instruments. Consequently, double beam spectrophotometers provide higher precision, especially important in quantitative analyses and long-term studies.

3. What precautions should be taken when using a double beam UV-Vis spectrophotometer ?

To ensure accurate and reliable measurements, several precautions are necessary. Proper calibration should be performed regularly to maintain wavelength and photometric accuracy. Cuvettes must be clean and free from scratches, as imperfections can scatter light and affect readings. Samples should be free of bubbles and particulates that can interfere with light transmission. The instrument's optical components should be kept clean, and the environment should be free from vibrations and temperature fluctuations. Additionally, allowing the instrument to warm up before use ensures stable light source output.

4. Why is baseline correction still necessary in a double beam UV Vis spectrophotometer ?

double beam UV Vis spectrophotometer systems correct for source and detector drift, baseline irregularities still arise due to slight differences in cuvette optics, beam path alignment, or lamp switching at UV–visible junctions. A baseline or “zero” correction—measuring both sample and reference with identical blanks—accounts for these residual offsets. This ensures that any absorbance reading truly reflects the analyte alone, not instrument artifacts. Without periodic baseline correction, spectral shape distortions or absorbance offsets may mimic real spectral features. Modern instruments often automate this step, simplifying workflows while preserving data integrity. Running a baseline correction is especially critical when using mismatched cuvette pairs or scanning across multiple lamp wavelengths.

5. What role does spectral bandwidth play in measurement accuracy in double beam UV-Vis spectrophotometer ?

It supplies advanced Double Beam UV-Vis spectrophotometer Spectral bandwidth-the wavelength range passing through the sample at once—must be narrow enough to resolve fine absorption peaks, especially for sharp, narrow-band analytes. If bandwidth exceeds peak width, absorbance readings will be lowered and broadened, compromising quantitative precision. However, narrowing bandwidth also reduces signal intensity and increases noise, requiring longer integration times. Research-grade double beam instruments often offer adjustable bandwidth (e.g. 0.1–2?nm), letting users optimize the balance between resolution and sensitivity. Calibrating bandwidth settings against known standards ensures accurate extinction coefficient determination—crucial for strict pharmacopoeial compliance.