Double Beam UV-Vis Spectrophotometer LUS-T2063

Introduction

Double Beam UV-Vis Spectrophotometer features a spectral wavelength of 2.0nm, ensuring reliable absorbance measurements across UV and visible ranges. It has dual beam optics for high accuracy and stable absorbance measurements. The spectrophotometer provides an advanced digital display, enhancing real-time data monitoring. Our double beam UV-visible spectrophotometer offers imported tungsten and deuterium lamps for enhanced light source stability and extended wavelength coverage.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Double Beam UV-Vis Spectrophotometer measures sample and reference light paths for stable absorbance analysis. It plays a key role in biochemical analysis, environmental monitoring, and pharmaceutical industries in quality control.

Specifications

Spectral Bandwidth	2.0nm
Wavelength Range	190 to 1100 nm
Optical System	Quasi double beam ratio monitoring optical path, holographic diffraction grating
Wavelength Accuracy	± 0.5nm
Wavelength Repeatability	0.2nm or less
Wavelength Setting	Automatic Wavelength Setting
Wavelength Resolution	0.1 nm
Luminosity Range	0 to 200% T, -0.3 to 3A, 0 to 9999 C, 0 to 9999 F
Photometric Accuracy	A (0 to 0.5A), 0.02mm ± 0.04 A 0.5 to1 (A), ±0.3%TT (0 to100%)
Photometric Repeatability	≤0.001A (0 to 0.5A), ≤0.002A (0.5 to 1A), T (0 to 100% to 0.15% or less)
Stray Light	≤0.05%T (220/360nm)
Baseline Drift	≤0.001A/h (500nm,0A)
Noise	± 0.1%T (0%T line) ±0.3%T (100%T line) ±0.0005A (500nm)
Baseline Flatness	±0.0015A
Scan Speed	High, medium and low gears are adjustable
Detector	Imported silicon photodiode
Light Source	Imported long-life tungsten and deuterium lamps
Display	6 "320×240 dot matrix display
Keyboard	Digital light touch film keys
Data Interface	Port
Printout	Microprinter, PC (online)
Power Supply	AC 90 to 250V, 50 /60Hz
Overall Dimensions	545×468 ×245mm
Weight	12 kg

Features

High Photometric Accuracy

Stable Baseline Performance

Automated Wavelength Calibration

Multiple Measurement Modes

Low Stray Light

FAQs

1. What is the fundamental working principle of a double beam UV-Vis spectrophotometer ?

double beam UV-Vis spectrophotometer operates by splitting a single light source into two separate beams: one directed through the sample and the other through a reference. This configuration allows simultaneous measurement of both beams, enabling real-time comparison and correction for fluctuations in light source intensity or detector sensitivity. The instrument uses a monochromator to isolate specific wavelengths of light, which then pass through the sample and reference paths. Detectors measure the intensity of transmitted light, and the instrument computes absorbance values based on the ratio of sample to reference intensities. This design enhances measurement accuracy and stability, particularly important in quantitative analyses. The double beam setup is advantageous for detecting low-concentration analytes and for applications requiring high precision.

2. How does a double beam spectrophotometer differ from a single beam spectrophotometer?

The primary distinction lies in the light path configuration. A single beam spectrophotometer measures the sample and reference sequentially, requiring manual intervention to switch between them, which can introduce errors due to lamp fluctuations or detector drift. In contrast, a double beam spectrophotometer simultaneously directs light through both the sample and reference, allowing real-time comparison and automatic correction for any variations. This results in improved accuracy, stability, and ease of use, especially for long-term measurements or when analyzing multiple samples. Additionally, double beam instruments often feature advanced optics and automated capabilities, making them suitable for high-throughput laboratories.

3. What are the key components of a double beam UV-Vis spectrophotometer ?

double beam UV-Vis spectrophotometer comprises several essential components: a light source (typically a deuterium lamp for UV and a tungsten lamp for visible light), a monochromator to select specific wavelengths, beam splitters or choppers to divide the light into sample and reference paths, cuvette holders for the sample and reference solutions, detectors (such as photodiodes or photomultiplier tubes) to measure transmitted light, and a data processing system to compute absorbance values. The design ensures that both beams traverse similar optical paths, minimizing discrepancies and enhancing measurement reliability.

4. How is wavelength accuracy ensured in a double beam UV-Vis spectrophotometer ?

Wavelength accuracy is critical for reliable spectrophotometric measurements. Calibration is typically performed using standard reference materials with known absorbance peaks, such as holmium oxide solutions. The instrument scans these standards, and the observed peak positions are compared to certified values. Any discrepancies are adjusted through the instrument's software or hardware settings. Regular calibration, as part of routine maintenance, ensures that the spectrophotometer provides accurate and reproducible wavelength readings, essential for quantitative analyses and method validation.

5. What is the procedure for calibrating a double beam UV-Vis spectrophotometer ?

It supplies premium double beam UV-Vis spectrophotometer . Calibration involves several steps to verify the instrument's performance parameters. First, the wavelength accuracy is checked using standards like holmium oxide, ensuring that the instrument correctly identifies known absorbance peaks. Next, the photometric accuracy is assessed using solutions with known absorbance values, such as potassium dichromate. Stray light is evaluated by measuring absorbance at wavelengths where the sample should ideally absorb no light, using solutions like potassium chloride. Baseline stability and noise levels are also monitored. Regular calibration, following standard operating procedures, maintains the instrument's accuracy and compliance with analytical standards.