Introduction
A double beam UV-visible spectrophotometer is an analytical instrument used to measure the absorption and the transmittance of the light by the samples over the ultraviolet and the visible wavelength regions, with the double beam optical design, and it is used in the chemistry, the biology, the clinical, the pharmaceutical, and the many other laboratories. The double beam UV-visible spectrophotometer measures the absorbance of the light by the sample, with the light beam split into the sample beam and the reference beam, and the ratio of the intensities measured, which compensates for the fluctuations of the light source and the drift, providing the stable and the accurate measurements; the double beam UV-visible spectrophotometer consists of a light source, a monochromator, a beam splitter, the sample and the reference compartments, a detector, and a control and a data analysis system, and it provides features such as the wavelength scanning, the kinetic measurements, and the standard curve analysis; the double beam UV-visible spectrophotometer is used for the quantitative and the qualitative analysis, for the measurement of the concentration of the samples, for the analysis of the nucleic acids and the proteins, for the quality control, for the research, and for many other applications, and it is an essential instrument for the UV-visible analysis. Double beam UV-visible spectrophotometers are used in the chemistry and the biology laboratories, in the clinical laboratories, in the pharmaceutical industry, in the food industry, and for many other applications. With their double beam design, double beam UV-visible spectrophotometers are essential for the accurate absorbance measurements.
Double Beam UV-Visible Spectrophotometer T-S221DBS
Double Beam UV-Visible Spectrophotometer is adopted with microcomputer technology and electronic control system, features holographic grating with 1200 lines/mm. Equipped with solid silic...
Double Beam UV-Visible Spectrophotometer T-S220DBS
Double Beam UV-Visible Spectrophotometer is a compact, tabletop, double beam and Czerny-Turner monochromator comprised Double Beam UV-Visible Spectrophotometer, comes with 20 mm of focal ...
Double Beam UV-Visible Spectrophotometer T-S213DBS
Double Beam UV-Visible Spectrophotometer is a light intensity measuring unit with deuterium lamp for prevention of ozone inhalation. The SiO2 coated optical mirror enables uninterrupted p...
Double Beam Uv-Visible Spectrophotometer T-S214DBS
Double Beam UV-Visible Spectrophotometer is a light intensity measuring unit with deuterium lamp for prevention of ozone inhalation. The SiO2 coated optical mirror enables uninterrupted p...
Double Beam UV-Visible Spectrophotometer T-S210DBS
Double beam UV-Visible Spectrophotometer is a double beam light intensity measuring unit with wavelength range 190 nm ~ 1100 nm. Tungsten and deuterium lamp as a light source with automat...
Double Beam UV-Visible Spectrophotometer T-S211DBS
Double Beam UV-Visible Spectrophotometer features a wavelength range of 190 to 1100 nm, ensuring precise and accurate spectral analysis of samples. It has double beam optical system and u...
Double Beam UV-Visible Spectrophotometer T-S212DBS
Double beam UV-Visible Spectrophotometer is a double beam light intensity measuring unit with wavelength range 190 nm ~ 1100 nm.It is equipped with Czerny Turner monochromator with reduce...
Standards
GB/T 26813-2010-Double-beam ultraviolet-visible spectrophotometers
European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible
ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
USP General Chapter <857>-Ultraviolet-Visible Spectroscopy
Complies with the above standards, but is not limited to them.
Features
1. Double-beam UV-visible spectrophotometer for accurate absorbance and transmittance measurement.
2. Dual-beam optical system compensates for light source drift and improves measurement stability.
3. Wide wavelength range with high resolution supports scanning and fixed-wavelength analysis.
4. Software supports spectral scanning, quantitation, kinetics and multi-wavelength measurement.
5. Suitable for pharmaceutical, chemical, environmental and educational laboratory applications.
FAQ
Q1: What testing scenarios is this instrument mainly applied to?
A: The double-beam UV-visible spectrophotometer is used in pharmaceutical, chemical, environmental, food and educational laboratories for quantitative and qualitative analysis by absorbance measurement in the ultraviolet and visible ranges. It supports drug content assay, impurity testing, enzyme activity measurement, nucleic acid and protein quantitation, water quality analysis and spectral scanning for compound identification. The double-beam design provides superior stability for long-duration scans and kinetic measurements by automatically compensating for light source intensity drift, making it suitable for regulated pharmaceutical testing and research requiring high accuracy.
Q2: What standards does the instrument comply with?
A: The instrument supports analytical methods referenced in USP, EP and ChP monographs, ASTM E169 for UV-Vis quantitative analysis, ISO 15529 for optical properties, and relevant GB/T standards for spectrophotometry. The hardware complies with IEC 61010 laboratory safety standards. Wavelength and absorbance accuracy should be verified with certified reference materials.
Q3: What is the warranty period for the whole machine and key components?
A: The whole spectrophotometer is covered by a 12-month warranty. The optical system, detector, light source and controller are covered, while lamps, cuvettes and calibration standards are consumables excluded from warranty. Extended warranty is available.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained analysts familiar with spectrophotometric principles. We provide operation manuals, application notes and structured training. Routine fixed-wavelength quantitation can be performed by trained operators with software wizards and standard procedures.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Wavelength and absorbance calibration with certified reference materials should be performed every 6-12 months for accredited laboratories. Routine verification before each analytical batch is recommended for regulated work. Lamp replacement should follow the manufacturer schedule.
Q7: Are supporting consumables universal?
A: Lamps, cuvettes and calibration standards are available from multiple qualified suppliers, though original lamps are recommended for optimal performance. Long-term consumable cost is moderate.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes wavelength range, resolution, sample compartment accessories and software modules. Additional accessories such as sippers, temperature controllers and microvolume adapters can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Standard delivery includes the spectrophotometer and accessories with an operation manual. Remote commissioning guidance is provided. On-site installation and training are not included for standard orders but can be arranged for laboratory setup projects.
Q10: What types of samples are compatible with this equipment?
A: The instrument analyzes liquid samples within the wavelength and absorbance range. Samples must be clear and homogeneous; turbid samples may cause scattering errors. Highly corrosive samples can damage cuvettes and require special material selection. Very high absorbance samples may require dilution to stay within the linear range.
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