Vertical Electrophoresis System LVES-T2010

Introduction

Vertical Electrophoresis System a glass plate of 216 × 110 mm, providing sample space for high-quality gel electrophoresis. It ensures fast gel plate installation, minimizes setup time, and enhances user convenience. The secure lid button design guarantees a tight seal, preventing leakage and ensuring safe operation. It supports multi-gel preparation capability, allowing simultaneous processing of multiple samples for increased efficiency. Our system delivers high-resolution gel separation, enabling precise analysis of nucleic acids and proteins.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Vertical Electrophoresis System  is used to separate proteins or nucleic acids based on their size and charge through a vertical gel matrix. It is used in molecular biology, and clinical research for protein analysis, / separation, and genetic studies.

Specifications

Glass Plate Dimensions	216 × 110 mm
Gel Dimensions	186 × 95 mm
Gel Thickness	1.0 mm
Number of Gels	1 to 2 pcs
Maximum Buffer Volume	1800 ml
Sample Throughput	25 to 104
Dimension (L × W × H)	290 × 170 × 155 mm
Weight	4.1 kg

Features

Auto Lid Switch-Off

Stable pH with Cooling

Safe Lid Button Design

Multi-Gel Modular Design

Quick Glass Plate Installation

FAQs

1. How is a vertical system different from a horizontal electrophoresis system?

The key difference lies in the gel orientation and separation applications. Vertical Electrophoresis System is commonly used for polyacrylamide gels, ideal for protein separation in applications like - and Western blotting, whereas horizontal systems use agarose gels primarily for and separation. In a vertical system, the gel is sandwiched between two glass plates and placed vertically, offering better resolution for protein samples.

2. What types of gels can be used with Vertical Electrophoresis System?

Vertical Electrophoresis System supports polyacrylamide gels () for separating proteins and other biomolecules and native gels for studying protein complexes and nucleic acids. Both hand-cast and commercially available pre-cast gels can be used with system, allowing flexibility in experimental protocols. Hand-cast gels allow users to customize the gel composition and thickness, whereas pre-cast gels provide convenience and consistency.

3. How many samples can Vertical Electrophoresis System handle in one run?

Vertical Electrophoresis System is highly versatile and can handle multiple sample configurations in a single run, typically supporting 10 to 15 wells per gel, depending on the comb size and format. dual-gel compatibility allows for simultaneous processing of two gels, doubling throughput without compromising performance. This feature is especially useful for high-throughput research applications where multiple samples need to be analyzed concurrently.

4. What buffer systems are recommended for Vertical Electrophoresis System?

It recommends using Tris-Glycine, Tris-Tricine, and Bis-Tris buffer systems based on the specific application. Tris-Glycine buffers are commonly used for - and native , while Tris-Tricine buffers are suitable for the separation of low molecular weight proteins. Bis-Tris buffers offer superior separation of proteins with narrow molecular weight ranges. It provides detailed buffer preparation protocols to ensure consistent ion strength and pH, which are essential for achieving reproducible and high-resolution results in electrophoresis runs.

5. How does temperature control during electrophoresis runs?

Temperature control is critical for preventing overheating and distortion of the gel. Vertical Electrophoresis System is equipped with effective heat dissipation mechanisms, including a heat-resistant design and a cooling system that maintains stable running temperatures. For high-voltage or extended runs, It recommends using an external cooling system or an ice bath to regulate the buffer temperature and prevent the gel from overheating. This ensures that samples migrate consistently, maintaining the integrity of the separation process.

6. How does system prevent buffer leakage during runs?

Vertical Electrophoresis System is equipped with leak-proof gel cassettes and buffer chambers that prevent leakage during electrophoresis runs. The system features a robust sealing mechanism with durable gaskets and clamping plates that maintain a tight seal throughout the process. This design helps maintain buffer consistency, minimizes ion depletion, and ensures optimal gel performance, reducing variability in results.

7. Can Vertical Electrophoresis System be used for Western blot transfer?

Yes, Vertical Electrophoresis System is fully compatible with Western blot transfer protocols. After electrophoresis, proteins can be transferred onto nitrocellulose or membranes using compatible blotting systems. It ensures efficient and uniform transfer, preserving protein integrity for downstream immunodetection and analysis. The system’s design supports both wet and semi-dry transfer methods, allowing researchers to choose the most suitable protocol for their application.