Introduction

Gradient thermal cycler enabled with Peltier heating units which can process more than 100 cycles and store data in the internal flash memory. It can accommodate 96×0.2 ml +77×0.5 ml sample in wells. User-friendly programming is achieved via 8-inches TFT color touch-screen with graphical display and windows operations system. This device can be applied across a temperature range of 0 to 100 °C with maximum ramping rate is 4.5°C/s. It is advantageous to use pre-installed 11 standard protocols and secure his data using password Login method. The files can be transferred via USB, printer support

Manufactured under strict quality control processes, complying with international laboratory equipment standards. Provides 1-year warranty for main components and lifetime technical support.

Applications

Used in Gene expression analysis, Pathogen detection, DNA methylation analysis, Genotyping and gene scanning, miRNA research etc

Widely applied in academic research laboratories, quality control departments of manufacturing enterprises, and testing institutions. Suitable for sample preparation, analysis, and storage in fields such as life sciences, materials science, environmental monitoring, and food safety.

Specifications

Capacity 96×0.2 ml+77×0.5 ml
Temperature Range 0 to 100 °C
Maximum. Heating Rate 4.5 °C/s
Maximum. Cooling Rate 4 °C/s
Uniformity ≤ ± 0.2 °C
Accuracy ≤ ± 0.1 °C
Display Resolution 0.1 °C
Temperature Control BlockTube
Ramping Rate Adjustable 0.1 to 4.5 °C
Gradient Uniformity ≤ ± 0.2 °C
Gradient Accuracy ≤ ± 0.2 °C
Gradient Temperature. Range 30 to 100 °C
Gradient Spread 1 to 30 °C
Hot Lid Temperature 30 to 110 °C
Hot Lid Height Adjustable Step less Adjustable
Number Of Programs 10000 +(USB FLASH)
Maximum. No. Of Step 30
Maximum. No. Of Cycle 100
Increment/Decrement Time 1 Second to 600 Second
Increment/Decrement Temperature 0.1 to 10.0 °C
Pause Function Yes
Auto Data Protection Yes
Holding 4 °C
Print Yes
LAN To Computer Yes
LCD 8 inch,800×600 pels, 260000 colors
Interface USB2.0 , LAN
Dimensions(L×W×H) 390×270×255mm
Weight 8.5 kg
Power Consumption 600 W
Power Supply 85 to 264 VAC , 47 to 63Hz

Features

Uniform heating with 3 different temperature of the samples is achieved with Peltier heating units with more than 100 cycles
It is well built with anodizing technology with aluminum block treatment which makes the durable and corrosion resistant
Maximum heating rate is 4.5 °C/s with temperature range of 0 to 100 °C
It has the capability to accommodate 96×0.2ml+77×0.5ml with moveable hot lid which can fit different sizes of tubes
It has the internal flash memory to store the 10000 files data for analysis
The connectivity between computer is via USB port and mini printer and can store the GLP data after every step to get reliable results
All the operations are controlled by a computer software system which instrument PCR protocols and also has in built 11 standard protocols
It has power of operation recovery option which helps in continuing the same command after a recovering from power cut
The user can access to his individual experimental data by setting a password and data management system
To satisfy the customers need it is installed with special feature like the user can add sample while the process is going on to encounter different variations
The user will be sent notifications via email about the process to alert when operation ends

FAQs

1. What is a Gradient Thermal Cycler

The Gradient Thermal Cycler is a high-performance PCR machine designed for precise temperature control and gradient functionality, enabling efficient DNA amplification for various molecular biology applications.

2. How does a Gradient Thermal Cycler work?

Gradient Thermal Cycler operates on the principle of thermal cycling, using precise temperature changes to facilitate DNA denaturation, primer annealing, and extension during PCR.

3. Is Gradient Thermal Cycler applicable in agricultural biotechnology?

Is Gradient Thermal Cycler used to detect genetically modified organisms (GMOs) and plant pathogens.

4. Can Gradient Thermal Cycler cycler be used in forensic DNA analysis?

Gradient Thermal Cycler amplifies STR markers from forensic samples for identity verification.

5. Is Gradient Thermal Cycler suitable for pathogen detection?

Gradient Thermal Cycler amplifies pathogen-specific DNA or RNA to identify infectious agents.

6. Is Gradient Thermal Cycler effective for detecting bacterial DNA?

Gradient Thermal Cycler amplifies specific bacterial genes for identification and antibiotic resistance testing.

7. Can Gradient Thermal Cycler device detect viruses in clinical samples?

Gradient Thermal Cycler is used to amplify viral RNA or DNA for diagnosis of infections like HIV, hepatitis, or COVID-19.

8. Can Gradient Thermal Cycler be used in pharmaceutical quality control?

Gradient Thermal Cycler detects microbial contamination or verifies genetic components in drug production.

9. Is Gradient Thermal Cycler applicable for CRISPR-related studies?

Gradient Thermal Cycler helps verify genome edits by amplifying target regions before sequencing.

10. What kind of maintenance does Gradient Thermal Cycler require?

Routine maintenance includes cleaning the thermal block, checking lid pressure, updating software, inspecting components, calibrating temperature, and keeping vents dust-free to ensure optimal performance.

11. What is a Gradient Thermal Cycler?

A Gradient Thermal Cycler is a PCR machine designed for simultaneous testing of different annealing temperatures across a temperature gradient.

12. How does Gradient Thermal Cycler work?

It works by creating a temperature gradient across the block, enabling multiple PCR reactions to be run at slightly different temperatures in the same cycle.

13. Can Gradient Thermal Cycler be used for multiplex PCR?

It is ideal for multiplex PCR, as it helps determine a temperature that works efficiently for multiple primer sets in the same reaction.

14. How does Gradient Thermal Cycler improve PCR efficiency in research labs?

It improves PCR efficiency in research labs by reducing trial-and-error cycles and enabling faster optimization of reaction conditions.

15. Is Gradient Thermal Cycler useful in genotyping applications?

It is widely used in genotyping to optimize conditions for amplifying specific alleles or polymorphisms accurately.

16. How does Gradient Thermal Cycler support environmental DNA (eDNA) analysis?

It supports eDNA analysis by enabling rapid optimization of PCR conditions for complex, low-abundance DNA targets in environmental samples.

17. Is Gradient Thermal Cycler beneficial for forensic DNA analysis?

It is highly beneficial for forensic DNA analysis, where precise optimization is required to amplify minute or degraded DNA samples reliably.

18. How does Gradient Thermal Cycler support microbiome or metagenomics studies?

It supports microbiome and metagenomics studies by enabling precise amplification of complex microbial DNA mixtures with optimized conditions.

19. Can Gradient Thermal Cycler be used for pathogen detection in food safety testing?

It is commonly used in food safety testing to optimize PCR conditions for detecting bacterial, viral, or fungal pathogens in food samples.

20. How does Gradient Thermal Cycler support molecular diagnostics in clinical labs?

It supports molecular diagnostics by allowing rapid and accurate PCR optimization for disease-specific genetic markers.

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