Introduction
A liquid nitrogen freezer is a storage unit that uses liquid nitrogen to maintain an extremely low temperature, typically about -196 degrees Celsius, for the long-term storage and preservation of biological samples, and it is used in biobanks, research laboratories, clinical laboratories, and reproductive medicine for the storage of cells, tissues, embryos, sperm, and other biological materials. The freezer consists of a heavily insulated storage vessel, such as a Dewar, that holds the liquid nitrogen, and racks or canisters for the storage of the samples; the samples are stored in the vapor phase above the liquid nitrogen or immersed in the liquid nitrogen, at the ultra-low temperature that effectively stops all biological activity and preserves the samples for long periods, and the freezer maintains the low temperature automatically by the evaporation of the liquid nitrogen, with automatic refilling systems in many models, and it has a digital temperature display, alarms, and locks, and the samples are handled with proper safety precautions, since liquid nitrogen is extremely cold. Liquid nitrogen freezers are used for the long-term storage of cell lines, for the storage of tissues and organs for research and transplantation, for the storage of embryos, sperm, and eggs in reproductive medicine, for the storage of microorganisms, for biobanking, for the storage of vaccines and biological products, and for many other applications, and they are available in various capacities, from small laboratory dewars to large storage tanks, with features such as automatic refilling, alarms, and sample management systems. With their ultra-low temperatures, liquid nitrogen freezers are essential for the long-term preservation of biological materials.
Liquid Nitrogen Freezer LLNF-T2012
is a single-door cryogenic storage device. It operates on working temperature of -196 ℃ to +120 ℃ and has temperature control precision of ± 3 ℃. Designed with liquid nitrogen refrig...
Liquid Nitrogen Freezer LLNF-T2010
is a double door unit, made using food grade 304 stainless steel, thermal insulation. Special design of door seal with an automatic heating system, ensures convenient opening of the door...
Liquid Nitrogen Freezer LLNF-T2011
is a double door unit, made using food grade 304 stainless steel, thermal insulation. Special design of door seal with an automatic heating system, ensures convenient opening of the door...
Liquid Nitrogen Freezer LLNF-T2010
Liquid Nitrogen Freezer is a single door unit, which is suitable to freeze the small amount of food. This liquid nitrogen cabinet freezer is made using food grade 304 stainless steel, th...
Liquid Nitrogen Freezer LLNF-T2011
Liquid Nitrogen Freezer offers freezing capacity is about 200 kg per hour, making it suitable for industrial-scale food processing applications. It uses liquid nitrogen as the freezing m...
Liquid nitrogen freezer LLNF-T2012
has a capacity of 200 kg/h and an adjustable working temperature of ～-150℃. It is designed with 304 stainless steel thermal protection coating and thermal insulation. It is equipped with...
Standards
IEC 61010-2-011:2019-Particular requirements for refrigerating equipment
IEC 62552-1:2015-Household refrigerating appliances-Characteristics and test methods-Part 1: General requirements
ISO 20387:2018-Biotechnology-Biobanking-General requirements for biobanking
ISO/TS 20388:2021-Biotechnology-Biobanking-Requirements for animal biological material
Complies with the above standards, but is not limited to them.
Features
1. Liquid nitrogen freezer for cryogenic storage at -196 degrees C (liquid phase) or -190 degrees C (vapor phase).
2. Liquid nitrogen cooling for long-term cryopreservation of biological samples.
3. Suitable for biobanking, clinical, stem cell, pharmaceutical, biological and research labs.
4. Includes cryogenic tank, storage racks, liquid nitrogen supply, monitor and manual.
5. Supports long-term cryopreservation of cells, tissues, embryos and biological samples.
FAQ
Q1: What testing scenarios is this freezer mainly applied to?
A: The liquid nitrogen freezer is used in biobanks, clinical laboratories, stem cell banks, pharmaceutical storage, biological research, hospitals, fertility clinics and research to provide cryogenic storage at -196 degrees C (liquid phase) or -190 degrees C (vapor phase) using liquid nitrogen for long-term cryopreservation of biological samples including cells, tissues, embryos, stem cells, DNA, RNA, proteins and other precious biological materials. It supports long-term biobanking and sample archiving, stem cell and embryo cryopreservation (fertility clinics, regenerative medicine), clinical specimen storage, pharmaceutical biological product storage, cell line and microorganism preservation, research sample storage, and precious biological material preservation. The liquid nitrogen temperature (-196 degrees C) effectively stops all molecular motion and degradation, providing indefinite long-term storage of viable biological materials.
Q2: What standards does the freezer comply with?
A: The liquid nitrogen freezer complies with relevant ISO, GB/T standards for cryogenic storage equipment, applicable medical device regulations for clinical/pharmaceutical use, and relevant cryogenic safety standards. The hardware complies with applicable safety standards for cryogenic equipment. Liquid nitrogen handling follows applicable safety regulations (OSHA, GB). Biobanking follows ISBER best practices and AABB/FACT standards for stem cells/embryos. Users should verify compliance with specific facility requirements for cryogenic storage, including oxygen monitoring and ventilation.
Q3: What is the warranty period for the whole freezer and key components?
A: The whole liquid nitrogen freezer is covered by a 12-month warranty. The cryogenic tank, storage racks, monitor, controller and housing are covered, while storage boxes, canisters, liquid nitrogen and sensors are consumables or accessories. Damage from improper use, physical impact or unauthorized repair is excluded. Extended service contracts are available for this critical cryogenic storage equipment. Vacuum jacket warranty may have separate terms (typically 5-10 years).
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires specially trained personnel familiar with cryogenic storage, liquid nitrogen handling and safety. We provide comprehensive training, operation manuals and application support. Proper sample handling (cryogloves, face shield), liquid nitrogen filling, level monitoring, temperature monitoring, alarm response, inventory management and emergency procedures are critical for safe operation and sample protection. Liquid nitrogen can cause severe cryogenic burns and oxygen deficiency.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Temperature monitor calibration against a reference recommended every 6-12 months. Liquid nitrogen level sensor verification periodically. Alarm system tested. Vacuum jacket condition checked (frost on outside indicates vacuum loss). Routine inspection of tank and racks. Full system performance verification annually. Oxygen monitor calibration per schedule (critical for safety).
Q7: Are supporting consumables universal?
A: Storage boxes, canisters, cryogloves, face shields, liquid nitrogen and sensors are available from cryogenic suppliers, though box/canister size should match the tank. Long-term consumable cost includes liquid nitrogen (continuous consumption) and depends on tank size, usage and ambient temperature.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes capacity (number of samples/vials), storage type (liquid phase, vapor phase), storage configuration (boxes, canisters, racks), monitoring (temperature, level, alarms), data logging, remote monitoring, auto-fill capability and accessories. Additional storage boxes, racks and safety equipment can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, liquid nitrogen freezers include on-site installation, commissioning, safety assessment and operator training by certified cryogenic specialists. Site preparation guidance is provided before delivery, including liquid nitrogen supply, oxygen monitoring and ventilation requirements.
Q10: What types of samples are compatible with this equipment?
A: The liquid nitrogen freezer stores biological samples including cells, tissues, embryos, stem cells, microorganisms, DNA, RNA, proteins, specimens and pharmaceuticals requiring long-term cryogenic storage at -196 degrees C (liquid) or -190 degrees C (vapor). Samples should be in appropriate cryovials or cryo-bags rated for liquid nitrogen temperature, properly sealed and labeled (labels should be cryogenic-resistant). The freezer should not be overloaded. Users should maintain proper liquid nitrogen level (never let samples warm above -135 degrees C), monitor temperature continuously with alarms, use proper cryogenic PPE (cryogloves, face shield, lab coat) when handling samples and liquid nitrogen, ensure proper ventilation and oxygen monitoring in the storage room (oxygen deficiency hazard), implement sample inventory management (location tracking), have emergency procedures for liquid nitrogen spill or freezer failure, follow ISBER/AABB best practices for biobanking, and for clinical use, follow applicable regulatory requirements and chain of custody. The freezer should be placed in a well-ventilated room with oxygen monitor and alarm (liquid nitrogen evaporation can displace oxygen). Liquid nitrogen should be supplied by a qualified vendor with proper safety procedures. The vacuum jacket should be inspected regularly for frost (indicates vacuum loss). Samples should be stored in the vapor phase (-190 degrees C) if cross-contamination from liquid nitrogen is a concern (liquid phase can allow pathogen transmission via contaminated liquid nitrogen). For valuable samples, replication in multiple freezers is recommended. The freezer should have backup liquid nitrogen supply or auto-fill system. For transport of cryogenic samples, use proper dry shippers (validated for transport). Liquid nitrogen dewars and freezers should be handled carefully to avoid tipping or damage. The storage room should have emergency oxygen supply and proper exhaust. All personnel working with liquid nitrogen should be trained in cryogenic safety and first aid for cryogenic burns.
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