Introduction
High performance liquid chromatography (HPLC) is an analytical technique used for the separation, the identification, and the quantification of the components in the mixtures, and it is used in the pharmaceutical, the clinical, the food, the environmental, the chemical, and the many other applications. In the HPLC, the sample is dissolved in the mobile phase and pumped through a column packed with the stationary phase, and the components are separated based on their interactions with the stationary phase, and they are detected as they elute from the column, providing the chromatogram; the HPLC system consists of a solvent delivery system with the pump, an injector, a separation column, a detector, such as the UV-visible, the fluorescence, or the refractive index detector, and a data system, and it provides features such as the gradient elution, the high resolution, and the automation; the HPLC is used for the analysis of the pharmaceuticals and the drugs, for the analysis of the food and the beverages, for the analysis of the environmental samples, for the analysis of the biomolecules, for the quality control, for the research, and for many other applications, and it is an essential analytical technique in the modern laboratories. HPLC systems are used in the pharmaceutical industry, in the clinical laboratories, in the food industry, in the environmental laboratories, in the chemical industry, and for many other applications. With their separation, HPLC systems are essential for the analysis of the complex mixtures.
High Performance Liquid Chromatography T-S221HPLC
High Performance Liquid Chromatography delivers a flow rate range of 0.001 to 10.000 mL/min, ensuring stable and accurate liquid transfer. It operates within a temperature range of 0 to 4...
High Performance Liquid Chromatography T-S210HPLC
High Performance Liquid Chromatography features a precise solvent flow range of 0.001 to 10.000 mL/min. It features a UV detector with a wavelength range of 190 nm to 700 nm and a deuteri...
Standards
USP General Chapter <621>-Chromatography
European Pharmacopoeia 2.2.46-Chromatographic separation techniques
WHO TRS 1025 Annex 4-Good chromatography practices
Complies with the above standards, but is not limited to them.
Features
1. High-performance liquid chromatography system for separation and quantitative analysis of compounds.
2. Modular design including pump, autosampler, column oven and detector for flexible configuration.
3. High-pressure binary or quaternary gradient pump delivers precise and reproducible mobile phase flow.
4. Supports UV-Vis, fluorescence, refractive index and mass spectrometry detection options.
5. Software supports method development, quantitation, regulatory compliance and data management.
FAQ
Q1: What testing scenarios is this instrument mainly applied to?
A: The HPLC system is used in pharmaceutical, chemical, environmental, food and clinical laboratories for separation, identification and quantitation of compounds in complex mixtures. It supports drug content and impurity analysis, pesticide residue testing in food, environmental pollutant monitoring, clinical biomarker measurement, natural product analysis and quality control of chemical products. The high-resolution separation capability makes it the gold standard for quantitative analysis of non-volatile, thermally labile compounds that cannot be analyzed by gas chromatography, with applications spanning R&D, quality control and regulatory testing.
Q2: What standards does the instrument comply with?
A: The system supports analytical methods referenced in USP, EP and ChP monographs, ISO and ASTM standards for specific analytes, and relevant GB/T standards for HPLC analysis. The hardware complies with IEC 61010 laboratory safety standards. Electronic records may be configured for 21 CFR Part 11 compliance. System suitability testing follows pharmacopoeial requirements.
Q3: What is the warranty period for the whole machine and key components?
A: Standard warranty is 12 months from commissioning. The pump, autosampler, detector and control system are covered, while columns, solvents, vials and seals are consumables excluded from warranty. Extended warranty and service contracts are available for this high-value system. Damage from improper solvent use or lack of maintenance is excluded.
Q4: Is this device difficult to operate? Is professional training required?
A: HPLC operation requires specialist training in chromatography principles, method development and instrument maintenance. We provide structured operator training, application support and method consultation. Routine use by trained analysts is supported by software wizards and standard operating procedures. Method development for complex samples requires experienced chromatographers.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: System suitability testing with reference standards should be performed before each analytical batch for regulated methods. Pump flow rate, detector wavelength and autosampler precision should be calibrated every 6-12 months. Annual preventive maintenance by a certified engineer is strongly recommended to maintain performance and extend column and seal life.
Q7: Are supporting consumables universal? What about the long-term procurement cost?
A: HPLC columns, solvents, vials and filters are available from multiple qualified suppliers, though original columns and instrument-specific seals are recommended for optimal performance. Long-term consumable and service cost is significant and should be included in the annual laboratory budget. Solvent and column costs dominate recurring expenses.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes pump type, detector configuration, autosampler capacity, column oven range and software modules. Custom orders require detailed application discussion and extend lead-time. Users should define target analytes, matrices, sensitivity requirements and throughput before configuration.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, HPLC systems include on-site installation, commissioning and operator training by a certified application specialist. Site preparation guidance is provided before delivery to ensure the laboratory meets power, solvent storage and ventilation requirements.
Q10: What types of samples are compatible with this equipment?
A: The system analyzes liquid-soluble organic and some inorganic compounds within the column and detector capabilities. Samples must be filtered and free of particulate matter to protect the column and system. Highly viscous, strongly acidic or basic samples may require dilution or special columns. Compounds that are volatile or thermally stable may be better suited to GC analysis. Users should verify column chemistry and mobile phase compatibility for each application.
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