Performance inspection for Class II biological safety cabinets is implemented following biosafety standards. Key indicators including airflow velocity, airflow pattern, HEPA filter integrity, negative pressure sealing performance, noise level and illuminance are tested comprehensively. Standard testing methods are adopted to evaluate the biological protection performance of cabinets in biological experiments and microbiological research, safeguard operators, test samples and ambient environment, and meet the safety management requirements of biosafety laboratories.
Inspection Items
Airflow pattern, inflow airflow velocity, product protection performance, illuminance, drain pan leakage, HEPA filter integrity, temperature rise, cross-contamination protection, downflow airflow velocity, personnel protection performance, exterior inspection, safety alarm function, ultraviolet lamp inspection, noise level, ultraviolet irradiation intensity, cabinet leakage, airflow direction, alarm and interlock system, wind speed, average airflow velocity at work opening, glove port wind speed, cabinet air supply volume, static pressure difference inside cabinet, HEPA filter leak test, cleanliness class, leak rate of HEPA/ULPA filters, airflow smoke pattern test, ultraviolet radiation intensity, structural inspection, air volume, exterior material inspection, height of front operating opening, ultraviolet radiation test, cabinet leakage prevention, vibration test, ambient humidity adaptability, ambient temperature adaptability, cleanability, disinfection feasibility, airflow fluctuation alarm, cabinet anti-tilt performance, support feet and casters inspection, exhaust alarm for Class II A1/A2 cabinets, wind speed display function, sampling port inspection, drain pan inspection, interlock alarm for internal supply/exhaust fans, workbench anti-deformation performance, stability test, personnel/product/cross-contamination protection, motor and fan performance
Inspection Standards
1. YY 0569-2011 Class II Biological Safety Cabinets
2. JJF 1815-2020 Calibration Specification for Class II Biological Safety Cabinets, Clause 7.1
3. SN/T 3901-2014 Specification for Use and Management of Biological Safety Cabinets, Appendix B Clause 4.1.1
4. GB 50346-2011 Technical Code for Biosafety Laboratory Buildings
5. GB 19489-2008 Laboratories - General Requirements for Biosafety
6. ISO 14644-1:2015 Cleanrooms and Associated Controlled Environments - Part 1: Classification of Air Cleanliness by Particle Concentration
7. NSF/ANSI 49-2024 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification
8. EN 12469:2000 Biotechnology - Performance Criteria for Microbiological Safety Cabinets
Inspection Equipment
Airflow velocity tester, differential pressure gauge, HEPA filter integrity tester, noise meter, illuminance meter, particle counter, airflow visualization smoke generator
Key Indicators and Inspection Requirements
1. Airflow velocity: downflow velocity 0.38±0.025 m/s, inflow velocity 0.57±0.025 m/s
2. Airflow direction: no reverse flow, fluctuation ≤5%
3. Sealing performance: pressure decay ≤10% per minute under 500 Pa
4. Cleanliness class: not lower than ISO Class 5
5. Noise level: ≤67 dB(A)
6. Illuminance: ≥350 lx
Inspection Methods and Implementation Points
1. All tests are carried out in accordance with GB 19489-2008
2. Airflow velocity: grid measurement with a hot-wire anemometer
3. Airflow direction verification: smoke generator for flow visualization
4. Sealing test: pressure decay method
5. Cleanliness monitoring: particle counter sampling
6. Noise measurement: A-weighted sound level recording
Precautions
1. Fully clean and disinfect the cabinet interior prior to testing
2. Eliminate ambient airflow interference in the test area
3. Ensure the anemometer meets grade 2 or higher calibration standard
4. Fully close all door and opening interfaces of the cabinet
5. Restrict unnecessary personnel movement during testing
6. Carry out rectification and retest for non-compliant units
Note: All information above is for reference only. For further details and customized inspection solutions, please contact us at any time.
