Vertical Electrophoresis System LVES-T2013

Introduction

Vertical Electrophoresis System features a glass plate dimension of 216 × 220 mm, providing sample space for high-resolution gel separation. It includes an auto lid switch-off mechanism that automatically powers down the system when the lid is open, enhancing user safety. The stable pH with cooling ensures consistent results by maintaining optimal conditions throughout the process. It’s safe lid button design prevents accidental openings, adding an extra layer of protection during operations. Our system allows for quick glass plate installation, minimizing setup time and streamlining workflows.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Vertical Electrophoresis System  is used to separate proteins or nucleic acids based on their size and charge through a vertical gel matrix. It is used in molecular biology, and clinical research for protein analysis, / separation, and genetic studies.

Specifications

Glass Plate Dimensions	216 × 220 mm
Gel Dimensions	186 × 205 mm
Gel Thickness	1.0 mm
Number of Gels	1 to 2 pcs
Maximum Buffer Volume	3500 ml
Sample Throughput	25 to 104
Dimension (L × W × H)	290 × 170 × 250 mm
Weight	5.6 kg

Features

Low Buffer Consumption

Enhanced Sample Loading Precision

Heat Dissipation Efficiency

Corrosion-Resistant Components

Minimal Sample Leakage Risk

FAQs

1. How do the electrodes in Vertical Electrophoresis System ensure uniform migration?

Vertical Electrophoresis System is equipped with high-quality platinum electrodes that ensure uniform electric field distribution across the gel. These electrodes minimize voltage fluctuations and maintain consistent current flow, which is essential for the even migration of proteins or nucleic acids. The design reduces potential artifacts and ensures accurate and reproducible band patterns. system also provides easy electrode alignment and replacement, allowing for hassle-free maintenance and reliable performance.

2. What should be done if migration is inconsistent or bands appear distorted?

If migration is inconsistent or bands appear distorted in Vertical Electrophoresis System, several factors should be checked. This includes ensuring that the gel is properly polymerized, the buffer is fresh, and the voltage settings are appropriate for the sample type. Additionally, verifying that the comb was inserted correctly and that there are no air bubbles in the gel can prevent irregularities. It provides troubleshooting guidelines and user manuals to help identify and rectify such issues, ensuring smooth and consistent runs.

3. Can Vertical Electrophoresis System be configured for two-dimensional electrophoresis (2D-)?

Yes, Vertical Electrophoresis System can be configured for two-dimensional electrophoresis (2D-), allowing for the separation of complex protein mixtures. In 2D-, the first dimension involves isoelectric focusing (), followed by - in the second dimension. system supports the integration of modules and provides detailed instructions on preparing and running 2D gels, enabling researchers to obtain high-resolution separation of proteins based on their isoelectric points and molecular weights.

4. Can different comb sizes be used in Vertical Electrophoresis System to adjust sample loading capacity?

Yes, Vertical Electrophoresis System offers multiple comb size options to accommodate varying sample volumes and experimental needs. Users can choose combs with different well configurations to load a higher or lower number of samples per gel. The system’s flexible design allows researchers to optimize their workflows by selecting the appropriate comb size based on the number of samples and desired resolution. combs are made from high-quality materials, ensuring durability and consistent sample loading.

5. How can researchers ensure proper gel polymerization in a Vertical Electrophoresis System?

To ensure proper gel polymerization in Vertical Electrophoresis System, researchers should follow the recommended protocols for preparing and casting gels. This includes using fresh acrylamide and bisacrylamide solutions, maintaining the correct concentration ratios, and adding polymerization catalysts such as and ammonium persulfate () in precise amounts. system offers a leak-proof casting assembly that facilitates uniform polymerization and prevents gel deformation. Clear guidelines provided by It help users achieve consistent and high-quality gels every time.

6. How can air bubbles be avoided while pouring gels in Vertical Electrophoresis System?

Air bubbles can affect gel integrity and disrupt sample migration. To avoid air bubbles when pouring gels in Vertical Electrophoresis System, it is essential to degas the acrylamide solution before pouring and to pour the gel slowly along the sides of the casting unit. casting clamps and spacers are designed to minimize the formation of bubbles during polymerization, ensuring smooth and uniform gels. It also provides user manuals with step-by-step instructions to help researchers avoid common pitfalls during gel preparation.

7. How can band resolution be improved in Vertical Electrophoresis System?

To improve band resolution when using Vertical Electrophoresis System, researchers should optimize several factors, including gel concentration, buffer quality, and voltage settings. Using gradient gels can also enhance resolution by providing better separation of molecules with similar molecular weights. system ensures uniform current flow and consistent voltage delivery, reducing band smearing and distortion. Detailed troubleshooting guidelines provided by It help users identify potential issues and optimize conditions for achieving sharp and well-defined bands.