Introduction

Inverted Fluorescence Microscope includes motorized autofocusing mechanism with 0.1μm repeat positioning accuracy for clear imaging. It provides a magnification range of 100× to 400×, ensuring detailed fluorescence observation. The NIS60 infinite optical system enhances clarity and resolution for accurate analysis. A motorized 6-hole nosepiece with a slot allows transmitted and reflected imaging. Designed for fluorescence imaging, phase contrast studies, and live cell analysis in research laboratories.

This microscope is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Our Inverted Fluorescence Microscope is ideal for observing cells and microorganisms, supporting continuous monitoring and capturing images for cytology, immunology, and genetic research.

Specifications

Viewing Head Trinocular head with Bertrand lens, inclined at 45°, Interpupillary 47-78mm, 3-position beam split ratio: 50/50, 100/0, 0/100
Optical System NIS60 Infinite optical system
Eyepiece SW10×/25mm, φ30mm
Objective Infinite Plan semi- phase contrast objective with:
10× NA=0.3, WD=8.1mm, Cover glass 1.2mm
20× NA=0.45, WD=7.5 to 8.8mm, Cover glass 0 to 2mm
40× NA=0.60, WD=3 to 4.4mm, Cover glass 0 to 2mm
Nosepiece Motorized 6-hole nosepiece with slot (for transmitted and reflected)
Condenser Motorized long working distance condenser, NA 0.55, WD 26mm, with 6-position plate
Transmitted Illumination 5W illumination (service life: minimum 50,000 hours)
Focusing Motorized auto-focus, independent left/right hand wheels, three-speed adjustment, focusing range 9mm, repeat positioning accuracy: 0.1μm, motorized escape and recovery
Internal Magnification 1×, 1.5×
Side Video Port Switchable by turning plate, 3 modes:
Left side port/eyepiece = 50/50
Right side port/eyepiece = 20/80
Left & right side port/eyepiece = 0/100
Stage Motorized three-layer mechanical stage, size: 340×230mm, movement range: 130(X)×100(Y)mm, flexible knob, attachable small stages
Fluorescent Attachment Epi-fluorescence with 5W lamp, B/G/UV filters (100V), field diaphragm, center adjustable
Motorized Filter Block Multi-model, 6 positions, removable from the main frame for easy cube replacement
C-mount Adapter 1× C-mount Adapter

Features

Motorized condenser adjustment

Trinocular head with Bertrand lens

Long working distance condenser

Epi-fluorescence illumination

Motorized three-layer stage

Multiple magnification settings

Independent focus control

Switchable side video port

Removable filter cube design

FAQs

1. What are the key factors to consider when selecting this microscope for laboratory use?

When selecting this microscope, key factors include: 1) The instrument's precision and accuracy to meet experimental requirements; 2) Compatibility with the types of samples and applications you will be working with; 3) Ease of use and maintenance requirements; 4) Safety features to protect operators and samples; 5) Calibration and certification requirements; 6) Cost-effectiveness and long-term reliability; 7) Technical support and service availability from the supplier. Evaluating these factors ensures that the instrument meets your laboratory's specific needs.

2. How often should this microscope be calibrated to ensure accurate results?

It is recommended to calibrate this microscope at least once every 6-12 months under normal usage conditions. More frequent calibration (every 3-6 months) may be necessary if: 1) The instrument is used continuously for extended periods; 2) It is exposed to harsh environmental conditions (temperature fluctuations, humidity, dust); 3) It is used for critical applications requiring high precision; 4) The instrument has been moved or subjected to physical shock; 5) Calibration checks indicate significant deviations from standard values. Always follow the manufacturer's calibration guidelines.

3. What are the common maintenance procedures required for this microscope?

Common maintenance procedures include: 1) Regular cleaning of the instrument's exterior and sample contact surfaces to prevent contamination and ensure proper operation; 2) Inspection of moving parts (if applicable) for wear and lubrication as recommended by the manufacturer; 3) Replacement of consumable parts (filters, bulbs, reagents) according to usage guidelines; 4) Verification of performance parameters (temperature, pressure, voltage) regularly; 5) Storage of the instrument in a clean, dry environment when not in use; 6) Keeping a maintenance log to track service activities and identify potential issues early; 7) Following the manufacturer's specific maintenance schedule for optimal performance and longevity.

4. Can this microscope be used with different types of samples, and are there any compatibility considerations?

Yes, this microscope can be used with a variety of sample types, but compatibility considerations are important: 1) Check if the instrument's materials are compatible with the chemical composition of your samples (avoid corrosive or reactive substances that may damage the instrument); 2) Ensure that the sample volume and concentration are within the instrument's recommended range; 3) For biological samples, consider sterility requirements and potential cross-contamination risks; 4) For volatile or hazardous samples, ensure proper ventilation and safety precautions are in place; 5) Consult the manufacturer's documentation for specific sample compatibility guidelines, and perform compatibility tests with new sample types before regular use.

5. What should be done if the microscope malfunctions or produces inconsistent results?

If malfunctions or inconsistent results occur: 1) First, check the instrument's user manual for troubleshooting guidelines specific to the issue; 2) Verify that the instrument is properly calibrated and within its calibration period; 3) Inspect for obvious issues such as loose connections, damaged parts, or incorrect settings; 4) Clean the instrument thoroughly to remove any contamination that may affect results; 5) Repeat the experiment with a known standard sample to determine if the issue is with the instrument or the sample; 6) If the problem persists, discontinue use of the instrument to prevent further damage or inaccurate results; 7) Contact technical support or a qualified service technician for diagnosis and repair, providing details of the issue and any troubleshooting steps already performed.

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