Introduction

Vertical Electrophoresis System features a glass plate dimension of 200 × 200 mm, offering sufficient space for accurate gel electrophoresis. It ensures error-free electrode connection, reducing the risk of faulty setups and ensuring consistent performance. The time-saving gel setup minimizes preparation time, enhancing overall laboratory efficiency. Its minimal heat generation prevents sample degradation, maintaining optimal conditions throughout the process. Our system supports flexible gel size options, allowing customization based on specific experimental needs.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Vertical Electrophoresis System  is used to separate proteins or nucleic acids based on their size and charge through a vertical gel matrix. It is used in molecular biology, and clinical research for protein analysis, / separation, and genetic studies.

Specifications

Glass Plate Dimensions 200 × 200 mm
Gel Dimensions 162 × 175 mm
Gel Thickness 1.0 mm
Number of Gels 1 to 2 pcs
Maximum Buffer Volume 4000 ml
Sample Throughput 21 to 70
Dimension (L × W × H) 295 × 170 × 279 mm
Weight 7.1 kg

Features

Rapid Gel Casting Process

Accurate Band Separation

Adjustable Running Parameters

Consistent Migration Pattern

Shockproof Lid Design

FAQs

1. Can different buffer systems be used in Vertical Electrophoresis System?

Yes, Vertical Electrophoresis System is compatible with various buffer systems, including Tris-Glycine, Tris-Tricine, and - buffers, depending on the type of electrophoresis being performed. The system allows flexibility in choosing the appropriate buffer to achieve optimal separation of proteins or nucleic acids. It provides detailed protocols and recommendations for buffer preparation, ensuring that users can select the right buffer system for their specific applications without compromising the quality of the results.

2. How is temperature managed during extended runs in Vertical Electrophoresis System?

Vertical Electrophoresis System includes temperature regulation features that prevent overheating during extended runs. Some models are equipped with cooling systems that maintain consistent temperatures, preventing thermal degradation of samples and buffer solutions. Additionally, system design facilitates efficient heat dissipation, ensuring that long-duration experiments are carried out without compromising the integrity of the samples. The system’s manuals include best practices for temperature management during electrophoresis.

3. How does Vertical Electrophoresis System ensure accurate and even sample loading?

Vertical Electrophoresis System is designed to ensure accurate and even sample loading by offering well-aligned combs and uniform gel wells. The system minimizes the risk of cross-contamination and sample leakage by maintaining precise comb placement during gel polymerization. It also provides a variety of comb configurations to accommodate different sample volumes, ensuring reproducibility and accuracy in band separation. Users can refer to detailed protocols for loading techniques and best practices.

4. How can users improve the interpretation of band patterns in Vertical Electrophoresis System?

To improve band pattern interpretation, Vertical Electrophoresis System provides consistent and reproducible migration patterns by ensuring uniform electric fields and high-quality gel casting. For enhanced visualization, It recommends using appropriate staining and destaining protocols. Additionally, It provides guidelines for optimizing running conditions and sample preparation, which helps researchers, achieve clear and distinct bands, making data interpretation more accurate and reliable.

5. How can users ensure proper alignment of gel plates in Vertical Electrophoresis System?

Proper alignment of gel plates is critical for preventing leakage and ensuring uniform gel thickness. Vertical Electrophoresis System is equipped with precision-aligned plate holders and casting clamps that maintain tight seals and prevent gel distortion. Users should follow instructions for assembling the gel plates, ensuring that spacers are evenly placed and that the clamps are securely fastened. maintenance guidelines also include regular checks for wear and tear in spacers and clamps to ensure smooth operation.

6. What safety precautions should be followed when using Vertical Electrophoresis System?

When using Vertical Electrophoresis System, users should adhere to standard laboratory safety protocols, including wearing protective gloves and goggles. Since the system involves the use of electrical currents and hazardous chemicals like acrylamide, It provides safety guidelines to minimize risks. The system also features insulated covers and secure clamps to prevent accidental contact with running buffers or electric fields. It encourages users to regularly inspect the system for leaks and faults to ensure safe operation.

7. Can Vertical Electrophoresis System be used for multiplexed gel electrophoresis?

Yes, Vertical Electrophoresis System can be configured for multiplexed gel electrophoresis, allowing multiple samples to be analyzed simultaneously. This feature is particularly useful in high-throughput research applications where multiple samples need to be compared under the same conditions. It offers combs with multiple well configurations to facilitate multiplexed analysis, ensuring consistent results and reducing variability between samples. Detailed instructions provided by It guide users in setting up and executing multiplexed runs effectively.

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