Fluorometer-LFFM-T2010

Introduction

Fluorometer is the next generation of benchtop dual-channel Fluorometer, features 365 ± 20 nm and 460 ± 20 nm excitation filter and measures dsDNA concentration with high sensitivity lower detection limit of 0.5 ng/ul. It offers UV and blue channel fluorescence to measure fluorescence intensity of fluorescent reagent that combines with target molecule to read concentration of sample. Designed to accurately detect high-sensitive fluorescence while quantifying , , and protein. This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Fluorometer is used for processing scarce and difficult samples, for low quantity of , , or protein after extraction, also, in expensive experiments: q , cloning, transfection, the next generation sequencing and etc. Applied for Nucleic acid quantification, Plant reporter gene detection, Apoptosis detection.

Specifications

Dynamic range	Five orders of magnitude
Linear dynamic range	R2 >0.995
Processing time	3s (Once)
Light source	UV Blue
Excitation filters	365 ± 20 nm 460 ± 20 nm
Emission filters	420 to 480 nm (60 nm) 525 to 570 nm (45 nm)
Detector	Photodiode
Repeatability	<1.5 %
Stability	<1.5 %
Sensitivity	ds : 0.5 ng/ml
Dimension	194 × 155 × 72.5 mm
Weight	0.4 kg

Features

Designed with 4.3 inch touch screen, small and easy to use

Easy measurement within 3 sec for , , and protein

High sensitivity lowest detection limit is 0.5 pg/ul

Linear dynamic range with five orders of magnitude

Equipped with two fluorescence channels for nucleic acid, protein quantitation in one detection

Offers opening system

Adopts tube adapter

It can save at most 1,000 data and output data by port

FAQs

1. What is the Fluorometer , and what makes it unique compared to conventional fluorometers?

The Fluorometer is a next-generation benchtop dual-channel fluorometer designed to measure , , and protein concentrations with high accuracy and sensitivity. It features dual excitation filters of 365 ± 20 nm (UV) and 460 ± 20 nm (blue), along with corresponding emission filters for precise detection of fluorescence signals. One of its unique aspects is the ability to detect double-stranded at a lower detection limit of 0.5 ng/µl, which makes it highly sensitive for molecular biology applications. Unlike conventional models, it provides rapid results with a processing time of just 3 seconds per reading, making it ideal for both routine laboratory use and advanced research.

2. What types of biomolecules can be quantified using the Fluorometer ?

The Fluorometer is versatile in its application and can be used to accurately quantify nucleic acids such as and , as well as proteins when used with compatible fluorescent dyes. Its dual-channel design allows detection of both UV and blue fluorescence signals, enabling a wide range of molecular biology assays. This makes it suitable for laboratories involved in genomics, proteomics, and other life science fields that require precise and sensitive quantification of biomolecules.

3. What are the sensitivity and detection capabilities of the Fluorometer ?

The Fluorometer is engineered for high-sensitivity detection with a lower detection limit of 0.5 ng/µl for dsDNA. It provides a dynamic range across five orders of magnitude, with a linear dynamic range correlation coefficient (R²) greater than 0.995. These features ensure reliable and reproducible quantification of samples across a wide concentration range. Combined with its repeatability and stability of less than 1.5%, the device guarantees consistent and accurate results even when handling low-concentration samples.

4. What features are not available in the Fluorometer models?

While the Fluorometer offers advanced features for fluorescence quantification, certain functionalities are not available in this model. These include endotoxin () detection, 2 to 3 calibration point settings, WiFi or Ethernet connectivity for network-based operation, and an 8-tube strip format for batch sample processing. Laboratories requiring these specialized functions may need to consider alternative models or complementary equipment that provides these advanced options.

5. What are the typical applications of the Fluorometer in research and diagnostics?

The Fluorometer is widely used in molecular biology, genetic research, and clinical diagnostics. It is ideal for quantifying nucleic acids in extraction and purification workflows, quality assessment, protein quantification, and fluorescence-based biochemical assays. Its high sensitivity and reproducibility make it suitable for next-generation sequencing sample preparation, analysis, gene expression studies, and other advanced research applications where precise quantification of biomolecules is essential.