Introduction

Gradient PCR Machine works within range of 4 to 99.9°C. Its high-speed heating and cooling rates ensure efficient thermal cycling. Its user-friendly interface enables real-time monitoring and seamless operation management. It features multiple login management and large data storage, for secure access and protocol retrieval. Gradient PCR Machine is ideal for molecular biology, diagnostics, and research settings.

Manufactured under strict quality control processes, complying with international laboratory equipment standards. Provides 1-year warranty for main components and lifetime technical support.

Applications

Gradient PCR Machine is ideal for Molecular Biology, Genetics, Clinical Diagnostics, Biotechnology & Research Labs for DNA/RNA amplification, sequencing, genetic disorder analysis, gene expression studies and mutation analysis.

Selection Guide

Gradient PCR Machine is a versatile and reliable system suitable for research studies involving gene amplification, mutation analysis, genotyping, and cloning. Its advanced gradient functionality enables simultaneous testing of multiple annealing temperatures, making it especially useful for protocol optimization and assay development. With a sample capacity of 96 × 0.2 ml wells, a wide temperature range of 4°C to 99.9°C, and accurate thermal control, it supports various PCR techniques, including standard, hot-start, and touchdown PCR. The model is commonly used in genomic, clinical, forensic, agricultural, and environmental research due to its efficiency and flexibility. Equipped with user-friendly software and touchscreen controls, it streamlines workflow in high-throughput labs. Certified with CE and ISO standards, the system ensures safety, quality, and performance, making it a dependable choice for researchers seeking accuracy and reproducibility in their experiments.

Specifications

Single Step Time	1 to 59m59s
Temperature Range	4 to 99.9℃
Sample Capacity	0.2ml × 96
Gradient	12
Gradient Temperature Uniformity	±0.3℃
Gradient Temperature Accuracy	±0.3℃
Gradient Temperature Range	30 to 99.9℃
Gradient Temperature Difference Range	0.1 to 30℃
Maximum Heating Rate	4.5℃/s
Maximum Cooling Rate	4℃/s
Temperature Uniformity	±0.25℃
Temperature Accuracy	±0.20℃
Temperature Display Resolution	0.1℃
Hot Cover Temperature Range	30 to 110℃
Maximum Steps of the Program	30
Program Max. Cycle Number	99 to 599
Time Increment/Decrement	599s
Temperature Increase/Decrease	-9.9 to 9.9℃
Insulation Temperature	16℃
Program Storage Quantity	Greater than 100
Power Consumption	600W
Power Supply	100 to 240V, 50/60Hz
Dimensions	205 × 280 × 160 mm
Net Weight	4.3kg

Features

Semiconductor technology

Touchscreen Display

Block/Tube Temperature Control

Program Pause Function

USB Connectivity

FAQs

1. What is Gradient PCR Machine

A Gradient PCR Machine is an apparatus designed to perform polymerase chain reaction (PCR) with a gradient function that allows simultaneous optimization of annealing temperatures.

2. Can Gradient PCR Machine be used in diagnostic applications?

Gradient PCR Machine is used in research-based diagnostic applications where temperature optimization and precise amplification are critical, such as pathogen detection or genotyping.

3. Can Gradient PCR Machine be used for cloning experiments?

Gradient PCR Machine is used for cloning experiments by enabling high-fidelity amplification of gene fragments needed for insertion into cloning vectors.

4. How does Gradient PCR Machine enhance reproducibility in PCR-based workflows?

Gradient PCR Machine enhances reproducibility in PCR-based workflows through its consistent temperature control, uniform heat distribution, and ability to optimize conditions before running full-scale reactions.

5. How does Gradient PCR Machine aid in microbial diversity analysis?

Gradient PCR Machine aids in microbial diversity analysis by allowing precise amplification of 16S rRNA under different temperature conditions to reduce bias and improve specificity.

6. Can Gradient PCR Machine be used for forensic DNA analysis?

Gradient PCR Machine can be used for forensic DNA analysis by enabling amplification of degraded or low-quantity DNA with optimized conditions that reduce nonspecific amplification.

7. How is Gradient PCR Machine beneficial in epigenetics research?

Gradient PCR Machine is beneficial in epigenetics research for optimizing bisulfite-treated DNA amplification, which often requires highly specific and sensitive conditions.

8. Can Gradient PCR Machine be used in gene fusion detection for oncology research?

Gradient PCR Machine is used in gene fusion detection for oncology research by fine-tuning annealing conditions to amplify fusion breakpoints in cancer-related genes.

9. How is Gradient PCR Machine applied in foodborne pathogen detection?

Gradient PCR Machine is applied in foodborne pathogen detection by optimizing conditions for rapid amplification of species-specific genes from pathogens like Salmonella, Listeria, and E. coli.

10. How is Gradient PCR Machine applied in microbial resistance profiling in wastewater?

Gradient PCR Machine is applied in microbial resistance profiling in wastewater by enabling efficient amplification of resistance genes and mobile elements under variable temperature conditions.
11. What is a Gradient PCR?

A Gradient PCR is an apparatus designed for identifying the optimal annealing temperature for a specific PCR reaction.
12. Is Gradient PCR faster than running multiple individual PCR reactions?

It can test multiple temperatures in one experiment thereby saving time and providing quick results.
13. Can Gradient PCR be used for different sets of primers?

It is used for testing different sets of primers to find the best annealing temperature for each set.
14. Is Gradient PCR suitable for all types of DNA?

It can be used with various types of DNA templates such as genomic DNA, plasmid DNA, and cDNA.
15. Can Gradient PCR help with low-abundance templates?

It can identify the best conditions for amplification of low-abundance templates thereby improving the chances of getting good results.
16. Can Gradient PCR be used for amplifying large DNA fragments?

It can be used to amplify large DNA fragments.
17. Can Gradient PCR be used for DNA amplification from environmental samples?

It can be used for amplifying DNA from environmental samples such as soil, water or biological samples.
18. Can Gradient PCR be used for diagnostic PCR assays?

It can be used for diagnostic PCR assays.