Introduction
Gradient Thermal Cycler is designed to accommodate 32 microtubes or 0.2 ml tube strips, providing versatile sample handling options. Offers a maximum heating rate of 5°C per second and a cooling rate of 4°C per second. Intuitive touchscreen allows easy program setup, real-time monitoring. Ensures optimal annealing temperatures for multiple reactions in a single run. Gradient Thermal Cycler is ideal for diagnostic laboratories focused pathogen detection, and genetic disease screening.
Manufactured under strict quality control processes, complying with international laboratory equipment standards. Provides 1-year warranty for main components and lifetime technical support.
Applications
Gradient Thermal Cycler is ideal for gene amplification, cloning, and sequencing.
Widely applied in academic research laboratories, quality control departments of manufacturing enterprises, and testing institutions. Suitable for sample preparation, analysis, and storage in fields such as life sciences, materials science, environmental monitoring, and food safety.
Specifications
| Capacity | 32 × 0.2 ml (microtubes), 4 × 8 × 0.2 ml (tube strips) |
| Gradient Temperature Range | 30℃ to105℃ |
| Maximum. Heating Rate | 5℃/s |
| Heating Lid Temperature Range | 30℃ to110℃ |
| Maximum. Cooling Rate | 4℃/s |
| Block Temperature Range | 4℃ to 105℃ |
| Gradient Temperature Accuracy | ±0.3℃ |
| Temperature Display Accuracy | 0.1℃ |
| Block Temperature Uniformity | ≤ ±0.25℃ |
| Gradient Temperature Uniformity | ±0.3℃ |
| Temperature Control Ways | Block or Tube |
| Temperature Increasing/Decreasing | 9.9 to 9.9℃ for Touchdown PCR |
| Block Temperature Accuracy | ±0.2℃ |
| Max. Cycles | 99 |
| Max. Steps | 30 |
| Timer (Long PCR) | 599 to 599 s increasing/decreasing |
| Timer (General) | 1s to 59min to 59sec/0 Infinite duration |
| Gradient Set Span | Max. 30℃ |
| Display | 1s to 59min to 59sec/0 Infinite duration |
| Stored Programs | Touch screen LCD |
| Number of Peltier Elements | 200 |
| Pause Function | 2 |
| Power Supply | Yes |
| Power Failure Recovery | AC110V to 220V, 50 to 60Hz |
| Power Consumption | Yes |
| Size | 225W |
| Net Weight | 243×172×145 mm |
Features
Touchscreen Interface
Multiple Block Format
Program Storage
Rapid Thermal Cycling
Durable
FAQs
1. What is a Gradient Thermal Cycler
A Gradient Thermal Cycler is a high-precision PCR machine that uses gradient temperature control to enable efficient DNA amplification in research and diagnostic applications.
2. How does a Gradient Thermal Cycler work?
A Gradient Thermal Cycler operates on the principle of Peltier thermoelectric heating and cooling, allowing precise thermal cycling for DNA amplification through PCR.
3. Can Gradient Thermal Cycler assist in CRISPR-based gene editing validation?
Gradient Thermal Cycler is used to amplify edited genomic regions for screening or sequencing.
4. Does Gradient Thermal Cycler support high-fidelity PCR needed for synthetic gene synthesis?
Gradient Thermal Cycler maintains consistent cycling conditions critical for high-fidelity DNA polymerases.
5. Is Gradient Thermal Cycler compatible with clinical viral load assays for HIV or hepatitis viruses?
Gradient Thermal Cycler is used in clinical labs for pre-qPCR viral load testing of common pathogens.
6. Can Gradient Thermal Cycler support quality control testing in pharmaceutical production?
Gradient Thermal Cycler amplifies DNA/RNA to monitor microbial contamination or verify genetic identity in biopharmaceuticals.
7. Is Gradient Thermal Cycler applicable for plant pathogen detection in crops?
Gradient Thermal Cycler amplifies DNA from viruses, fungi, or bacteria infecting agricultural samples.
8. Is Gradient Thermal Cycler used for validating genetically modified strains in fermentation or production lines?
Gradient Thermal Cycler helps confirm genetic identity and stability of engineered strains used in industrial bioprocesses.
9. Is Gradient Thermal Cycler suitable for allergen detection in processed food products?
Gradient Thermal Cycler is used to detect trace allergen DNA such as peanut, soy, or gluten markers.
10. What kind of maintenance does Gradient Thermal Cycler require?
Routine maintenance includes regular cleaning, software checks, proper ventilation, and periodic calibration.
11. What is a Gradient Thermal Cycler?
A Gradient Thermal Cycler is a PCR machine designed for simultaneous testing of different annealing temperatures across a temperature gradient.
12. How does Gradient Thermal Cycler work?
It works by creating a temperature gradient across the block, enabling multiple PCR reactions to be run at slightly different temperatures in the same cycle.
13. Can Gradient Thermal Cycler be used for multiplex PCR?
It is ideal for multiplex PCR, as it helps determine a temperature that works efficiently for multiple primer sets in the same reaction.
14. How does Gradient Thermal Cycler improve PCR efficiency in research labs?
It improves PCR efficiency in research labs by reducing trial-and-error cycles and enabling faster optimization of reaction conditions.
15. Is Gradient Thermal Cycler useful in genotyping applications?
It is widely used in genotyping to optimize conditions for amplifying specific alleles or polymorphisms accurately.
16. How does Gradient Thermal Cycler support environmental DNA (eDNA) analysis?
It supports eDNA analysis by enabling rapid optimization of PCR conditions for complex, low-abundance DNA targets in environmental samples.
17. Is Gradient Thermal Cycler beneficial for forensic DNA analysis?
It is highly beneficial for forensic DNA analysis, where precise optimization is required to amplify minute or degraded DNA samples reliably.
18. How does Gradient Thermal Cycler support microbiome or metagenomics studies?
It supports microbiome and metagenomics studies by enabling precise amplification of complex microbial DNA mixtures with optimized conditions.
19. Can Gradient Thermal Cycler be used for pathogen detection in food safety testing?
It is commonly used in food safety testing to optimize PCR conditions for detecting bacterial, viral, or fungal pathogens in food samples.
20. How does Gradient Thermal Cycler support molecular diagnostics in clinical labs?
It supports molecular diagnostics by allowing rapid and accurate PCR optimization for disease-specific genetic markers.
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