Uv vis spectrometer

Introduction

A UV Vis spectrometer is an analytical instrument used to measure the absorption and the transmittance of the light by the samples in the ultraviolet (UV) and the visible (Vis) wavelength regions, and it is used in the chemistry, the biology, the biochemistry, the clinical, and the many other industries for the analysis of the samples. The UV Vis spectrometer measures the absorbance of the light by the sample over the wavelength range, typically from about 190 to 1100 nanometers, covering the UV and the visible regions, and the spectrum provides the information about the electronic transitions of the molecules, and the concentration is determined by the Beer-Lambert law; the UV Vis spectrometer consists of a light source, a monochromator, a sample holder, a detector, and a control and a display unit, and it provides features such as the wavelength scanning, the multi-wavelength measurement, the kinetics, and the standard curve analysis; the UV Vis spectrometer is used for the quantitative and the qualitative analysis, for the measurement of the concentration of the solutions, for the analysis of the nucleic acids and the proteins, for the measurement of the enzyme activity, for the colorimetric analysis, for the quality control, and for many other applications, and it is available in various configurations, with the single-beam and the double-beam models; the UV Vis spectrometer is an essential instrument in the analytical laboratory. UV Vis spectrometers are used in the chemistry and the biology laboratories, in the clinical laboratories, in the pharmaceutical industry, in the food and the beverage industry, and for many other applications. With their UV Vis measurement, UV Vis spectrometers are essential for the comprehensive analysis.

UV-VIS Spectrometer LUVI-T2011

UV- Spectrometer offers a wavelength range of 240 to 900 nm, catering to measurements from ultraviolet to visible light. It is equipped with advanced a high-resolution linear detector an...

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UV-VIS Spectrometer LUVI-T2010

UV- Spectrometer offers a compact design that offers a wavelength range of 200 to 755nm. It features a fixed optical grating that maintains consistent signal stability during long-term u...

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Standards

European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible

ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers

USP General Chapter <857>-Ultraviolet-Visible Spectroscopy

Complies with the above standards, but is not limited to them.

Features

1. UV-Visible (UV-Vis) spectrometer for measuring absorption spectra across 190-1100 nm range.

2. Dual light source (deuterium + tungsten-halogen) with silicon detector for UV-Vis spectral analysis.

3. Suitable for chemical, biological, pharmaceutical, environmental, food, clinical and research labs.

4. Includes deuterium and tungsten lamps, monochromator, sample compartment, detector, software and manual.

5. Supports UV-Vis absorption spectroscopy for concentration measurement, kinetics and material characterization.

FAQ

Q1: What testing scenarios is this spectrometer mainly applied to?

A: The UV-Vis spectrometer is used in chemical analysis, biochemistry, molecular biology, pharmaceutical, environmental monitoring, food and beverage, clinical diagnostics, materials science and research to measure the absorption, transmission, or reflection spectra of samples across the ultraviolet (190-380 nm) and visible (380-780 nm) ranges for concentration determination, reaction kinetics, purity analysis, and material characterization. It supports chemical analysis (concentration of absorbing compounds, reaction kinetics, equilibrium studies), biochemistry (protein quantification (A280, Bradford, Lowry, BCA), nucleic acid quantification (A260, A260/A280 ratio), enzyme assays), pharmaceutical (API assay, impurity analysis, dissolution testing, content uniformity), environmental (water quality, heavy metals, nitrate, oil in water), and food (vitamins, color, preservatives).

Q2: What standards does the spectrometer comply with?

A: The UV-Vis spectrometer follows relevant ISO, ASTM, USP and GB/T standards including ISO 15519 (cuvettes), ASTM E131 (wavelength calibration), ASTM E2056 (multivariate calibration), USP <857> (UV-Vis spectrophotometry in pharmaceutical analysis), and GB/T standards for UV-Vis analysis. The hardware complies with IEC 61010 safety standards and EMC standards (IEC 61326). Calibration should use certified wavelength standards (holmium oxide glass, mercury-argon lamp) and photometric standards (NIST-traceable neutral density filters, potassium dichromate solution).

Q3: What is the warranty period for the whole spectrometer and key components?

A: The whole UV-Vis spectrometer is covered by a 12-month warranty. The deuterium lamp, tungsten-halogen lamp, monochromator, sample compartment, silicon detector, controller, software and housing are covered, while lamps (deuterium ~1000 hours, tungsten ~2000 hours), cuvettes (quartz for UV), calibration standards and fuses are consumables or accessories. Damage from improper use, lamp failure, optical contamination or unauthorized repair is excluded. Extended warranty may be available.

Q4: Is this device difficult to operate? Is professional training required?

A: Operation is relatively simple for basic use but requires trained personnel for accurate results and advanced applications. We provide operation manuals and training. Proper instrument preparation (warm-up 15-30 minutes, wavelength calibration, blank/reference measurement), cuvette handling (quartz for UV, matched set, wipe clear sides, no bubbles), measurement parameters (wavelength selection at lambda max, spectral scan, integration time), sample preparation (dilute to A=0.1-1.0, filter turbid samples), data interpretation (Beer-Lambert law, calibration curves, kinetics), and maintenance (lamp replacement, cleaning) are important for accurate results.

Q5: What is the typical lead-time for order delivery?

A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.

Q6: Is regular calibration required? What is the recommended calibration cycle?

A: Wavelength calibration recommended every 3-6 months using holmium oxide glass or mercury-argon lamp. Photometric/absorbance calibration every 6-12 months using NIST-traceable neutral density filters or potassium dichromate solution. Stray light check every 6-12 months (sodium nitrite solution). Routine wavelength and intensity verification before each use or per day. Full spectrometer performance verification annually. For regulated pharmaceutical use, follow USP <857> and GMP calibration schedules.

Q7: Are supporting consumables universal?

A: Lamps (deuterium D2, tungsten-halogen - specific to spectrometer model), cuvettes (quartz for UV-Vis, glass for Vis only, plastic disposable for Vis only; 1 cm standard, matched sets), calibration standards (holmium oxide glass, didymium glass, neutral density filters, potassium dichromate), sample holders (cuvette holder, test tube holder, sipper, auto-sampler, Peltier cell holder), printer and fuses are available from laboratory suppliers, though lamps and some accessories may be spectrometer-specific. Long-term consumable cost includes lamp replacement (deuterium lamps are more expensive) and cuvettes (breakable, quartz expensive).

Q8: Can I customize parameters or supporting accessories?

A: Customization includes instrument type (single-beam, double-beam, diode array), wavelength range (190-1100 nm standard, extend to 1400 nm with InGaAs), wavelength accuracy (+/- 0.1 to +/- 2 nm), photometric accuracy, resolution (0.1-5 nm), detector (silicon photodiode, CCD array, PMT), sample compartment accessories (auto-sampler, sipper, Peltier temperature controller, integrating sphere, solid sample holder, micro-volume adapter (for DNA/protein), fiber optic probe), software (basic, advanced (spectral scanning, kinetics, multi-wavelength, DNA/protein module, enzyme kinetics, data export, SDK, 21 CFR Part 11), language and accessories. Additional accessories can be ordered separately.

Q9: Does the purchase include on-site installation and commissioning service?

A: Standard delivery includes the spectrometer, power cord, dust cover, basic software, one pair of cuvettes (sometimes) and instruction manual. On-site installation, calibration and training are not included for standard orders but can be arranged at additional cost (recommended for double-beam, diode array, and regulated environments). Remote guidance is provided.

Q10: What types of samples are compatible with this equipment?

A: The UV-Vis spectrometer measures absorption/transmission of various samples including liquids (solutions, suspensions, emulsions - in quartz cuvettes for UV, glass for Vis only; clear homogeneous solutions for Beer-Lambert application; dilute to A=0.1-1.0; filter or centrifuge turbid samples), solids (thin films, coatings, glass, polymers, crystals - in solid sample holder or integrating sphere for diffuse reflectance), gases (in gas cells with quartz windows - UV-absorbing gases like ozone, NO2, SO2, benzene vapors), and biological samples (protein solutions (A280, colorimetric assays), nucleic acids (A260, A260/A280 purity ratio), cell suspensions (turbidity/OD600), enzyme assay mixtures) within the wavelength range (190-1100 nm), photometric range, and sample holder specification. Key considerations: UV measurements (below 380 nm) require quartz cuvettes (glass absorbs UV), deuterium lamp, and UV-transparent solvents (water, methanol, ethanol, acetonitrile - avoid solvents that absorb in UV (e.g., benzene, toluene, chloroform absorb strongly below 260 nm)); protein quantification (A280 for pure proteins with aromatic residues (Trp, Tyr, Cys); colorimetric assays (Bradford, Lowry, BCA) for complex samples; A205 for peptides); nucleic acid quantification (A260 for DNA/RNA concentration (1 A260 = 50 ug/mL dsDNA, 40 ug/mL RNA); A260/A280 ratio for purity (~1.8 for DNA, ~2.0 for RNA); A260/A230 ratio for contamination); Beer-Lambert linearity (A=0.1-1.0 optimal; above 1.0 stray light and nonlinearity cause errors; dilute concentrated samples); blank correction (always measure blank with same cuvette and solvent; for colorimetric assays, reagent blank); and wavelength selection (measure at lambda max for maximum sensitivity; verify with spectral scan if unknown).