Introduction
A UV-Vis spectrophotometer is an analytical instrument used to measure the absorbance or transmittance of light by a sample in the ultraviolet and visible wavelength regions, typically from about 190 to 1100 nanometers, and it is one of the most widely used instruments in chemistry, biochemistry, biology, clinical, pharmaceutical, and environmental laboratories. In operation, light from a source, such as a deuterium lamp for the UV region and a tungsten or halogen lamp for the visible region, is passed through a monochromator that selects the measurement wavelength, and the light beam passes through the sample in a cuvette; the instrument measures the intensity of the light transmitted through the sample relative to a reference, and the absorbance, which is related to the concentration of the absorbing species by the Beer-Lambert law, is displayed, and the instrument can perform wavelength scans, measuring the absorbance spectrum of the sample, and quantitative measurements at specific wavelengths. UV-Vis spectrophotometers are used for quantifying proteins, nucleic acids, and other biomolecules, for measuring the concentration of analytes in solutions, for colorimetric analysis, for enzyme assays, for monitoring reactions, for analyzing pharmaceuticals, for water and environmental analysis, for food analysis, for studying the optical properties of materials, and for many other applications, and they are available as benchtop instruments and portable models, with features such as wavelength scanning, multiple measurement modes, and data analysis software. With their versatility and importance, UV-Vis spectrophotometers are essential instruments for absorbance measurements.
Ultra Micro UV-Vis Spectrophotometer LMVS-T2011
Ultra Micro UV-Vis Spectrophotometer supports a minimum sample size of just 0.5 to 2 µL for applications requiring ultra-low volume analysis with high accuracy. It includes built-in tem...
Ultra Micro UV-Vis Spectrophotometer LMVS-T2010
Ultra Micro UV-Vis Spectrophotometer requires a minimum sample size of 0.5 to 2 µL for applications with limited sample volumes. It supports both micro-volume and cuvette-based measurem...
Ultra Micro UV-Vis Spectrophotometer LMVS-T2012
Ultra Micro UV-Vis Spectrophotometer operates with a minimal sample volume of 0.5 to 2 µL, making it ideal for analyzing small samples. It offers a wide detection concentration range fo...
Ultra Micro UV-Vis Spectrophotometer LMVS-T2011
Ultra Micro UV-Vis Spectrophotometer features a minimum sample size of 0.5µL for highly sensitive testing. It supports a wavelength range from 190 to 850 nm with a wavelength accuracy o...
Split Beam UV-Vis Spectrophotometer LUS-T2011
Split Beam UV-Vis Spectrophotometer a wavelength range of 190 to 1100 nm. It offers a wavelength accuracy of ±0.1 nm at 656.1nm and ±0.3 nm across all wavelengths, ensuring reliable re...
Ultra Micro UV-Vis Spectrophotometer LMVS-T2010
Ultra Micro UV-Vis Spectrophotometer offers extensive range from 190nm to 900nm, allowing for comprehensive sample analysis across broad spectrum. Its integrated advanced optical system ...
Split Beam UV-Vis Spectrophotometer LUS-T2010
Split Beam UV-Vis Spectrophotometer has an processed core and has a wavelength range of 190 to 1100 nm. Equipped with automatic wavelength setting and certain wavelength setting function...
Standards
European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible
ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
USP General Chapter <857>-Ultraviolet-Visible Spectroscopy
Complies with the above standards, but is not limited to them.
Features
1. UV-Vis spectrophotometer for measuring absorbance in ultraviolet and visible range.
2. Precision wavelength scanning for qualitative and quantitative spectroscopic analysis.
3. Suitable for chemical, biological, pharmaceutical, environmental, food and research labs.
4. Includes light source, monochromator, sample compartment, detector, software and manual.
5. Supports absorbance measurement for concentration, purity and kinetic analysis.
FAQ
Q1: What testing scenarios is this spectrophotometer mainly applied to?
A: The UV-Vis spectrophotometer is used in chemical analysis, biological research, pharmaceutical testing, environmental monitoring, food and beverage, clinical diagnostics, materials science and research to measure the absorbance of samples in the ultraviolet (190-400 nm) and visible (400-780 nm) wavelength range for quantitative concentration determination, purity assessment, kinetic studies and qualitative analysis. It supports pharmaceutical assay and impurity testing (drug concentration, purity), biological analysis (protein, DNA, enzyme activity), environmental water quality monitoring (nitrate, nitrite, heavy metals by colorimetry), food and beverage analysis (color, additives, contaminants), clinical chemistry analysis, material characterization, and general laboratory spectroscopic analysis. UV-Vis spectroscopy is one of the most widely used analytical techniques, providing fast, sensitive measurement for a wide range of applications.
Q2: What standards does the spectrophotometer comply with?
A: The UV-Vis spectrophotometer follows relevant ISO, ASTM and GB/T standards for UV-Vis spectrophotometry, including ISO 15529, ASTM E2728, and relevant pharmacopoeial methods (USP, EP, ChP). The hardware complies with IEC 61010 laboratory safety standards and IEC 61326 EMC standards. Calibration should use certified reference materials (e.g., holmium oxide, didymium filters, NIST-traceable standards) traceable to international standards. Pharmaceutical use follows GMP and data integrity requirements (21 CFR Part 11).
Q3: What is the warranty period for the whole machine and key components?
A: The whole UV-Vis spectrophotometer is covered by a 12-month warranty. The light source (deuterium, tungsten), monochromator, detector, electronics, software and housing are covered, while cuvettes, lamps and fuses are consumables. Damage from improper use or unauthorized repair is excluded. Extended service contracts are standard for this analytical instrument.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained analytical personnel familiar with UV-Vis spectroscopy, sample preparation and data interpretation. We provide operation manuals, training and application support. Proper wavelength calibration, blank correction, sample preparation, cuvette handling, method selection and result interpretation are important for accurate, reliable analysis.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Wavelength and absorbance calibration with certified reference materials recommended every 6-12 months. Routine verification before each analytical batch for regulated work. Light source (deuterium, tungsten) replacement per usage hours. Cuvette condition checked. Full system performance verification annually. Software updates and data integrity checks per regulatory requirements.
Q7: Are supporting consumables universal?
A: Cuvettes (glass, quartz, plastic), lamps, calibration standards and cleaning supplies are available from laboratory suppliers, though cuvette type should match the wavelength range (quartz for UV). Long-term consumable cost depends on sample volume and lamp usage.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes optical system (single beam, double beam), wavelength range, resolution, detector type, sample compartment accessories (sipper, microplate, Peltier), software features and accessories. Additional cuvettes, lamps and accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, UV-Vis spectrophotometers include on-site installation, commissioning, calibration and operator training by certified specialists. Site preparation guidance is provided before delivery.
Q10: What types of samples are compatible with this equipment?
A: The UV-Vis spectrophotometer measures absorbance of liquid samples in cuvettes (or solid samples with appropriate accessories) within the wavelength range, absorbance range and specification. Samples should be clear and homogeneous; turbid samples cause scattering and may require filtration or correction. Different wavelength ranges require specific cuvettes (quartz for UV below 350 nm, glass/plastic for visible only). Users should select appropriate wavelength for the analyte (lambda max), use matched cuvettes, perform proper blank correction (reagent blank), calibrate with certified standards, follow Beer-Lambert law for quantitative analysis (linear range), account for matrix interferences, report results with wavelength and method specified, and for pharmaceutical use, follow validated methods and pharmacopoeial requirements. The instrument should be allowed proper warm-up time (typically 15-30 minutes) before measurement. Cuvettes should be handled by the frosted sides only (not the optical surfaces) and cleaned properly between samples. For UV measurements, ensure the solvent does not absorb in the measurement range (use quartz cuvettes and UV-transparent solvents). For high-accuracy work, use double-beam instruments and proper temperature control. The light source should be replaced when intensity degrades (deuterium lamps typically last 1000-2000 hours). Waste should be disposed of per applicable regulations. For regulated use, maintain proper data integrity and audit trails per 21 CFR Part 11 / Annex 11.
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