Uv spectrometer

Introduction

A UV spectrometer is an analytical instrument used to measure the absorption and the transmittance of the light by the samples in the ultraviolet (UV) wavelength region, and it is used in the chemistry, the biology, the biochemistry, the pharmaceutical, and the many other industries for the analysis of the samples. The UV spectrometer measures the absorbance of the light by the sample over the UV wavelength range, typically from about 190 to 400 nanometers, and the spectrum provides the information about the electronic transitions of the molecules, which are related to the concentration and the structure of the absorbing species, and the concentration is determined by the Beer-Lambert law; the UV spectrometer consists of a light source, a monochromator, a sample holder, a detector, and a control and a display unit, and it provides features such as the wavelength scanning, the absorbance measurement, and the standard curve analysis; the UV spectrometer is used for the quantitative and the qualitative analysis of the compounds, for the measurement of the concentration of the solutions, for the analysis of the nucleic acids and the proteins, for the quality control, for the purity analysis, and for many other applications, and it is available as the benchtop and the portable instruments; the UV spectrometer is an essential instrument for the UV analysis. UV spectrometers are used in the chemistry and the biology laboratories, in the pharmaceutical industry, in the clinical laboratories, in the food industry, and for many other applications. With their UV measurement, UV spectrometers are essential for the analysis of the UV-absorbing compounds.

UV Spectrometer LUVSP-T2011

UV Spectrometer offers a lightweight design that covers the ultraviolet region with a wavelength range of 200 to 535 nm. It features a stable grating and supports a customizable slit, en...

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UV Spectrometer LUVSP-T2010

UV Spectrometer offers a compact design with a spectral range of 200 to 425 nm. It has a high-resolution linear detector and supports fast data acquisition via 2.0 connectivity. The ligh...

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Standards

European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible

ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers

Complies with the above standards, but is not limited to them.

Features

1. Ultraviolet (UV) spectrometer for measuring absorption spectra in the 190-400 nm range.

2. Deuterium light source with UV-optimized optics for ultraviolet spectral analysis.

3. Suitable for chemical, pharmaceutical, environmental, biological, food and research laboratories.

4. Includes deuterium lamp, monochromator, sample compartment, UV detector, controller, software and manual.

5. Supports UV absorption spectroscopy for compound identification, purity analysis and concentration measurement.

FAQ

Q1: What testing scenarios is this spectrometer mainly applied to?

A: The UV spectrometer is used in chemical analysis, pharmaceutical, environmental monitoring, biochemistry, food and beverage, cosmetics, and research to measure the absorption spectra of samples in the ultraviolet range (approximately 190-400 nm) for compound identification, purity analysis, concentration determination, and reaction monitoring. It supports pharmaceutical analysis (API identification and assay, impurity profiling, dissolution testing, content uniformity - many drug molecules absorb in UV), chemical analysis (aromatic compounds, conjugated systems, transition metal complexes, organic compound identification and purity), environmental monitoring (nitrate, nitrite, ozone, PAHs, pesticides, phenols in water and air), biochemistry (nucleic acid quantification (A260), protein quantification (A280), enzyme assays), food and beverage (vitamins (A, C, E), preservatives (benzoic acid, sorbic acid), colorants, antioxidants), and cosmetics (UV absorber analysis (sunscreen SPF testing), preservatives, fragrances).

Q2: What standards does the spectrometer comply with?

A: The UV spectrometer follows relevant ISO, ASTM, USP and GB/T standards including ASTM E131 (wavelength calibration), USP <857> (UV-Vis spectrophotometry), ISO standards for UV analysis, and GB/T standards for UV spectrophotometry. The hardware complies with IEC 61010 safety standards and EMC standards. Calibration should use certified wavelength standards (holmium oxide glass (UV absorption peaks at 241.7, 287.7, 361.5 nm), mercury-argon lamp (253.7 nm line)) and photometric standards (NIST-traceable potassium dichromate solution (absorbance at 235, 257, 313, 350 nm), neutral density filters).

Q3: What is the warranty period for the whole spectrometer and key components?

A: The whole UV spectrometer is covered by a 12-month warranty. The deuterium lamp, monochromator, sample compartment, UV detector (silicon photodiode or PMT), controller, software and housing are covered, while deuterium lamp (~1000 hour lifespan), quartz cuvettes, calibration standards and fuses are consumables or accessories. Damage from improper use, lamp failure, optical contamination or unauthorized repair is excluded. Extended warranty may be available.

Q4: Is this device difficult to operate? Is professional training required?

A: Operation requires trained personnel familiar with UV spectroscopy and proper technique. We provide operation manuals and training. Proper instrument preparation (warm-up 15-30 minutes for deuterium lamp stabilization, wavelength calibration, blank/reference measurement), cuvette handling (MUST use quartz cuvettes (glass absorbs UV below 340 nm), matched set, handle by frosted sides, wipe clear sides, no bubbles), measurement parameters (wavelength selection at lambda max, spectral scan range 190-400 nm, scan speed), sample preparation (use UV-transparent solvents (water, methanol, ethanol, acetonitrile; avoid benzene, toluene, chloroform which absorb strongly in UV), dilute to A=0.1-1.0, filter turbid samples), data interpretation (Beer-Lambert law, calibration curves, spectral library matching for identification), and maintenance (lamp replacement, cleaning) are important for accurate UV measurements.

Q5: What is the typical lead-time for order delivery?

A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.

Q6: Is regular calibration required? What is the recommended calibration cycle?

A: Wavelength calibration recommended every 3-6 months using holmium oxide glass or mercury-argon lamp (verify at 253.7 nm Hg line and holmium peaks). Photometric/absorbance calibration every 6-12 months using NIST-traceable potassium dichromate solution (verify at 235, 257, 313, 350 nm) or neutral density filters. Stray light check every 6-12 months (critical for UV - use sodium nitrite solution (absorbs all light below 400 nm) or cutoff filter; stray light is more problematic in UV due to higher photon energy and optical scattering). Routine wavelength and intensity verification before each use. Full spectrometer performance verification annually. For pharmaceutical use, follow USP <857> and GMP schedules.

Q7: Are supporting consumables universal?

A: Deuterium lamp (D2, specific to spectrometer model), quartz cuvettes (1 cm standard, matched sets; required for UV - glass cannot be used below 340 nm), calibration standards (holmium oxide glass, mercury-argon lamp, potassium dichromate standard solution), sample holders (cuvette holder, Peltier controller, sipper, auto-sampler, micro-volume adapter), printer and fuses are available from laboratory suppliers, though lamps and some accessories may be spectrometer-specific. Long-term consumable cost includes deuterium lamp replacement (expensive, ~1000 hour lifespan) and quartz cuvettes (more expensive than glass, breakable).

Q8: Can I customize parameters or supporting accessories?

A: Customization includes wavelength range (190-400 nm UV only, or extend to visible 190-700/1100 nm with tungsten lamp), wavelength accuracy, resolution (0.1-5 nm), detector type (silicon photodiode, photomultiplier tube (PMT) for higher sensitivity in far UV), instrument type (single-beam, double-beam), sample compartment accessories (auto-sampler, sipper, Peltier temperature controller, micro-volume adapter (for DNA/protein), fiber optic probe, integrating sphere), software (basic, advanced (spectral scanning, kinetics, DNA/protein module, spectral library, data export, SDK, 21 CFR Part 11), language and accessories. Additional accessories can be ordered separately.

Q9: Does the purchase include on-site installation and commissioning service?

A: Standard delivery includes the UV spectrometer, deuterium lamp, power cord, dust cover, basic software, one pair of quartz cuvettes (sometimes) and instruction manual. On-site installation, calibration and training are not included for standard orders but can be arranged at additional cost. Remote guidance is provided.

Q10: What types of samples are compatible with this equipment?

A: The UV spectrometer measures absorption of various samples including liquids (solutions of organic compounds with UV chromophores (aromatic rings, conjugated double bonds, carbonyl groups, transition metal complexes), pharmaceutical compounds, nucleic acids (A260), proteins (A280), environmental pollutants (nitrate, PAHs, pesticides), vitamins, preservatives - in quartz cuvettes; use UV-transparent solvents; dilute to A=0.1-1.0), solids (thin films, coatings, crystals, polymers - in solid sample holder; UV-transparent substrates (quartz, CaF2) for transmission measurement), gases (in gas cells with quartz or CaF2 windows - UV-absorbing gases (ozone, NO2, SO2, benzene, formaldehyde vapors)), and biological samples (nucleic acid solutions (DNA, RNA concentration and purity at A260/A280), protein solutions (A280 for pure proteins), cell-free extracts, enzyme assay mixtures) within the wavelength range (190-400 nm), photometric range, and sample holder specification. IMPORTANT UV-specific considerations: (1) Quartz cuvettes are REQUIRED (glass and plastic absorb UV below 340-350 nm; only quartz (fused silica) transmits down to 190 nm; disposable plastic cuvettes are only for Vis range). (2) Solvent selection is critical (water, methanol, ethanol, acetonitrile, hexane are UV-transparent down to ~190-210 nm; chloroform absorbs below 245 nm, benzene below 280 nm, toluene below 285 nm, DMSO below 265 nm - these cannot be used as solvents for UV measurement below their cutoff; always check solvent UV cutoff before use). (3) Far UV (190-220 nm) measurements are challenging (oxygen absorbs below 200 nm (Schumann-Runge bands), requiring nitrogen purging of sample compartment; many solvents and cuvette materials absorb in far UV; far UV is used for peptide bond absorption (A190-220) and some compound classes). (4) UV spectra provide compound identification information (chromophore type, conjugation length, aromatic substitution patterns) via spectral shape and lambda max, but UV alone is not definitive for identification (use with IR, MS, NMR for confirmation; spectral library matching can assist). (5) Purity analysis (UV can detect impurities with different chromophores (e.g., impurity with different lambda max appears as shoulder or peak in the spectrum; ratio of absorbances at two wavelengths can indicate purity (e.g., A260/A280 for nucleic acid purity)).