Introduction
A cryostat microtome is a precision instrument that combines a microtome with a freezing chamber, used to cut thin sections of frozen tissue specimens for microscopic examination, and it is used in histology, pathology, and research laboratories, especially for rapid diagnosis during surgery, known as frozen section analysis. The cryostat maintains the chamber at a low temperature, typically about -20 to -30 degrees Celsius, and the tissue specimen is frozen, embedded in a freezing medium, and mounted on the specimen holder; the microtome cuts sections of the frozen tissue, typically 5-10 micrometers thick, with the frozen tissue providing the rigidity needed for sectioning without embedding in paraffin, and the sections are collected on slides, fixed, and stained for microscopic examination; the cryostat allows the rapid preparation of sections for intraoperative diagnosis, and it is also used for histochemical and immunohistochemical procedures that require frozen sections, and the temperature is controlled to suit the tissue type. Cryostat microtomes are used for frozen section diagnosis in surgical pathology, for preparing sections for immunofluorescence and enzyme histochemistry, for research on fresh tissue, for preparing sections of fatty and other tissues that are difficult to section when embedded in paraffin, and for many other applications, and they are available as integrated instruments with the microtome and freezing chamber, with features such as temperature control, rapid freezing, and motorized or manual sectioning. With their rapid sectioning of frozen tissue, cryostat microtomes are essential for intraoperative diagnosis and research.
Cryostat Microtome LCRY-T2041
Cryostat Microtome features a frosting chamber that maintains a -10°C to -40°C range, ensuring optimal specimen integrity. It also offers an adjustable thickness range (1 to 100 µm) f...
Cryostat Microtome LCRY-T2021
Cryostat Microtome has a section thickness range that can be adjusted from 1 to 100 µm, and trimming thickness up to 600 µm. It combines an efficient freezing system with a semi-automa...
Cryostat Microtome LCRY-T2040
Cryostat Microtome features an adjustable thickness range (1.5µm increments) for ultra-thin, consistent tissue sections (5µm–100µm). It is the ultimate cutting-edge solution for high...
Cryostat Microtome LCRY-T2020
Cryostat Microtome is an enclosed and advanced semi-automatic microtome with adjustable section thickness range of 1µm to 100 µm. Equipped with freezing chamber of -10°C to -40°C tem...
Cryostat Microtome LCRY-T2030
Cryostat Microtome is a semi-automatic microprocessor-controlled microtome comprised with section thickness range of 1 to 100 µm. Features quick freezing shelf, -5°C to -35°C temperat...
Cryostat Microtome LCRY-T2010
Cryostat Microtome is a semi-automatic and advanced cryostat microtome comprised with retractable freezing chamber of -35°C to 40°C temperature range and quick-freezing shelf with -40...
Standards
ISO/TS 23824:2024-Medical laboratories-Guidance on application of ISO 15189 in anatomic pathology
Complies with the above standards, but is not limited to them.
Features
1. Cryostat microtome for sectioning frozen tissue samples at low temperature.
2. Integrated refrigerated chamber with microtome for frozen section preparation.
3. Suitable for pathology, histology, neuroscience, clinical and research laboratories.
4. Includes refrigerated chamber, microtome mechanism, blade holder, controller and manual.
5. Supports rapid frozen sectioning for intraoperative diagnosis and research.
FAQ
Q1: What testing scenarios is this microtome mainly applied to?
A: The cryostat microtome is used in surgical pathology, histology, neuroscience research, clinical diagnostics, pharmaceutical development and research to section frozen tissue samples at low temperature (typically -15 to -30 degrees C) for rapid histological examination, intraoperative diagnosis and research. It supports intraoperative frozen section diagnosis for surgical pathology, neuroscience brain slice preparation for research, clinical pathology rapid diagnosis, immunohistochemistry on frozen sections, and research requiring unfixed tissue sections. The cryostat combines refrigeration and microtomy in one unit, enabling rapid sectioning of frozen tissue without paraffin embedding, providing fast results for surgical decision-making.
Q2: What standards does the microtome comply with?
A: The cryostat microtome complies with relevant IEC 61010 laboratory electrical safety standards, applicable GB/T standards for histological and refrigeration equipment, and relevant medical device regulations for clinical use. The instrument follows applicable electromagnetic compatibility standards. Blades should be certified for histological use. Users should verify compliance with specific facility requirements including biosafety for potentially infectious tissue.
Q3: What is the warranty period for the whole machine and key components?
A: The whole cryostat microtome is covered by a 12-month warranty. The refrigeration system, microtome mechanism, controller, blade holder and housing are covered, while blades, disposable blade holders and fuses are consumables. Damage from improper use, blade damage or unauthorized repair is excluded. Extended service contracts are available for this clinical laboratory equipment.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained pathology or histology personnel familiar with frozen section technique, cryostat operation and biosafety. We provide comprehensive training, operation manuals and application support. Proper tissue embedding (OCT), temperature control, blade selection, section thickness, section handling, blade safety and biosafety procedures are critical for quality sections and safe operation.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Temperature calibration against a reference thermometer recommended every 6-12 months. Section thickness verification periodically. Blade holder and specimen holder alignment checked. Refrigeration system performance verified. Routine cleaning and decontamination recommended (critical for clinical use). Full system maintenance annually.
Q7: Are supporting consumables universal?
A: Cryostat blades, OCT compound, specimen holders, brushes and forceps are available from histology suppliers, though compatibility should be verified. Long-term consumable cost depends on sectioning volume and blade type.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes temperature range, section thickness range, chamber size, blade type, specimen holder, anti-roll plate, UV decontamination and accessories. Additional blade holders, specimen holders and accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, cryostat microtomes include on-site installation, commissioning, calibration and operator training by certified specialists. Site preparation including power and ventilation is recommended before delivery.
Q10: What types of samples are compatible with this equipment?
A: The cryostat microtome sections frozen tissue samples embedded in OCT (optimal cutting temperature) compound within the specimen size, section thickness and temperature specification. Different tissue types require specific sectioning temperatures (typically -15 to -30 degrees C, colder for fatty tissue). Tissues should be properly frozen (isopentane cooled with liquid nitrogen, or snap-frozen) to preserve morphology. Users should embed tissue properly in OCT, set appropriate chamber and specimen temperature, use sharp blades, use anti-roll plate for thin sections, follow proper biosafety procedures (potentially infectious tissue), decontaminate the chamber regularly, and handle sections carefully for mounting on slides. The cryostat should be allowed to reach operating temperature before use. For intraoperative diagnosis, rapid sectioning and staining protocols should be established. The chamber should be cleaned and decontaminated between specimens, especially for infectious cases. Waste should be disposed of per biological waste regulations. The refrigeration system should be maintained per manufacturer schedule, and the blade should be replaced when dull to prevent section compression or chatter.
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