Introduction
A single beam UV-Vis spectrophotometer is an analytical instrument that measures the absorption of ultraviolet and visible light by a sample using a single optical beam, and it is a simple, economical, and widely used instrument for quantitative analysis in chemistry, biochemistry, biology, and many other fields. In operation, light from a source, such as a deuterium lamp for the UV region and a tungsten-halogen lamp for the visible region, passes through a monochromator, which selects a specific wavelength, and then through the sample cell; the instrument measures the intensity of the light transmitted through the sample and compares it with a reference measurement taken with the blank or solvent, and calculates the absorbance, which is related to the concentration of the absorbing species by the Beer-Lambert law, and the measurement is performed at selected wavelengths or by scanning a wavelength range to produce an absorption spectrum. Single beam instruments are simpler and more affordable than double beam instruments, and they require the reference measurement to be taken separately before the sample measurement; they provide accurate results when operated carefully, and they are used for quantifying analytes in solutions, for determining the concentration of proteins, nucleic acids, and other compounds, for measuring the color of products, for monitoring reactions, for water and environmental analysis, for food and beverage quality control, and for educational purposes, in research, quality control, clinical, and teaching laboratories. Features include wavelength scanning or fixed-wavelength measurement, digital displays, and data output. With their simplicity and economy, single beam UV-Vis spectrophotometers are essential instruments for routine spectrophotometric analysis.
Single Beam UV-Visible Spectrophotometer LUS-T2040
Single Beam UV-Visible Spectrophotometer is equipped with advanced large display screen and 4 cell holder capacity. It has full functionality from 190 – 1100 nm range of wavelength. It ...
Single Beam UV-Visible Spectrophotometer LUS-T2011
Single Beam UV-Visible Spectrophotometer is formulated with large display screen and 8 cell holder capacity. It has full functionality from 190 – 1100 nm range of wavelength. It comes w...
Single Beam UV-Visible Spectrophotometer LUS-T2010
Single Beam UV-Visible Spectrophotometer is formulated with large display screen and 4 cell holder capacity. It has full functionality from 190 – 1100 nm range of wavelength. It comes w...
Single Beam UV-Visible Spectrophotometer LUS-T2052
Single Beam UV-Visible Spectrophotometer is bench top device with 190 nm to 1100 nm wavelength range, and offers spectral bandwidth of 0.5/1/2/4/5 nm. With T, A, C, E work mode it is des...
Single Beam UV-Visible Spectrophotometer LUS-T2051
Single Beam UV-Visible Spectrophotometer covers wavelength range of 190 nm to 1100 nm for versatile spectroscopic measurements. Its touchscreen display provides user-friendly navigation ...
Single Beam UV-Visible Spectrophotometer LUS-T2050
Single Beam UV-Visible Spectrophotometer offers optical system with 1200 lines/mm grating ensures high-precision spectral analysis. Features automatic wavelength calibration ensuring acc...
Standards
GB/T 26798-2010-Single-beam ultraviolet-visible spectrophotometers
European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible
ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
USP General Chapter <857>-Ultraviolet-Visible Spectroscopy
Complies with the above standards, but is not limited to them.
Features
1. Single-beam UV-Visible spectrophotometer for absorbance measurement.
2. Simple, reliable design with wavelength scanning for routine analysis.
3. Suitable for chemical, biological, pharmaceutical, food, educational and quality control.
4. Includes light source, monochromator, sample compartment, detector and software.
5. Supports concentration measurement, spectral scanning and routine quantitative analysis.
FAQ
Q1: What testing scenarios is this spectrophotometer mainly applied to?
A: The single-beam UV-Visible spectrophotometer is used in chemical analysis, biological research, pharmaceutical quality control, food testing, environmental monitoring, educational institutions and quality control laboratories to measure the absorbance of samples at ultraviolet and visible wavelengths for qualitative and quantitative analysis. It supports chemical compound concentration determination, nucleic acid and protein quantification, pharmaceutical content and purity testing, food additive and nutrient measurement, environmental pollutant analysis, educational chemistry and biology experiments, and routine quality control testing. The single-beam design provides a simple, reliable, cost-effective platform for routine absorbance measurement, making it ideal for teaching and standard analytical applications. It is essential for chemical, biological, pharmaceutical, food, environmental and educational laboratories requiring routine UV-Visible absorbance measurement for quantitative analysis, compound identification, quality control, educational experiments and research, where the single-beam design provides a simple, reliable, cost-effective approach to spectrophotometric analysis based on Beer-Lambert law, supporting chemical analysis, life science, pharmaceutical quality control, food testing, environmental monitoring and educational applications requiring accurate, reproducible absorbance measurement without the complexity of double-beam instruments.
Q2: What standards does the spectrophotometer comply with?
A: The single-beam UV-Visible spectrophotometer supports methods referenced in USP, EP and ChP monographs, ASTM E169 for UV-Vis quantitative analysis, ISO standards, and relevant GB/T standards. The hardware complies with IEC 61010 laboratory safety standards. Wavelength and absorbance accuracy should be verified with certified reference materials (holmium oxide, didymium filters).
Q3: What is the warranty period for the whole machine and key components?
A: The whole spectrophotometer is covered by a 12-month warranty. The light source, monochromator, detector, controller and software are covered, while lamps, cuvettes and calibration standards are consumables excluded from warranty. Damage from improper use or unauthorized repair is excluded. Extended service contracts are available.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained analysts familiar with spectrophotometric principles, sample handling and data interpretation. We provide operation manuals, training and application support. Proper wavelength selection, blank correction, sample handling, calibration and result interpretation are important for accurate analysis. Single-beam instruments require baseline correction before measurement.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Wavelength and absorbance calibration with certified reference materials recommended every 6-12 months. Routine verification before each analytical batch for regulated work. Light source replacement per usage hours. Full system performance verification annually. Sample compartment and optics cleaned per manufacturer schedule.
Q7: Are supporting consumables universal?
A: Lamps, cuvettes and calibration standards are available from multiple qualified suppliers. Long-term consumable cost is moderate depending on usage and light source type.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes wavelength range, resolution, detector type, sample compartment accessories, software features and automation. Additional accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Standard delivery includes the spectrophotometer and accessories with an operation manual. On-site installation, calibration and training are not included for standard orders but can be arranged for quality control laboratory setup. Remote guidance is provided.
Q10: What types of samples are compatible with this equipment?
A: The single-beam UV-Visible spectrophotometer analyzes liquid samples in cuvettes within the wavelength and absorbance range. Samples must be clear and homogeneous; turbid samples cause scattering errors. Highly corrosive samples require compatible cuvette material. Very high absorbance samples should be diluted to the linear range. Users should perform baseline/blank correction before measurement (important for single-beam), select appropriate wavelength for the analyte, use matched cuvettes, calibrate with certified standards, allow lamp warm-up, verify wavelength accuracy regularly, and follow Beer-Lambert law principles. For UV measurements below 300nm, quartz cuvettes are required (glass absorbs UV). The instrument should be allowed proper warm-up time, and baseline correction should be performed whenever the wavelength or lamp is changed for accurate single-beam measurement.
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