Introduction
A horizontal electrophoresis system is a laboratory apparatus used to separate macromolecules, such as nucleic acids (DNA and RNA) and proteins, by applying an electric field across a gel matrix in which the molecules migrate at different rates according to their size, charge, and shape, and it is a fundamental tool in molecular biology and biochemistry. The system consists of an electrophoresis tank or chamber, typically rectangular, containing a buffer solution, electrodes at each end, and a support for the gel, such as a casting tray with combs that form sample wells; the gel, usually agarose for nucleic acids or polyacrylamide for proteins, is cast in the tray and submerged in the buffer, samples are loaded into the wells, and an electric current is applied, causing the negatively charged molecules to migrate toward the positive electrode, with smaller molecules moving faster and farther than larger ones, so that the molecules are separated by size along the length of the gel. After electrophoresis, the separated molecules are visualized by staining, such as with ethidium bromide or other fluorescent dyes for nucleic acids, or by blotting and detection for proteins. Horizontal electrophoresis systems are used in molecular biology, genetics, and forensic laboratories for analyzing DNA fragments, for PCR product verification, for restriction enzyme analysis, for genotyping, for RNA analysis, and for protein separation, and they are available in various sizes, from mini systems for quick runs to large systems for high-throughput or preparative separations. Features include even electric field distribution, buffer recirculation, and safety interlocks. With their central role in nucleic acid analysis, horizontal electrophoresis systems are essential instruments in molecular biology laboratories.
Horizontal Electrophoresis System LHES-T2011
Horizontal Electrophoresis System has gel dimensions of 140 × 140 mm, 140 × 70 mm, and 70 × 70 mm and supports flexible gel formats to suit various lab needs. It ensures efficient buf...
Horizontal Electrophoresis System LHES-T2012
Horizontal Electrophoresis System gel dimensions of 130 × 130 mm, 78 × 100 mm, and 48 × 75 mm accommodates multiple gel sizes. It delivers high-resolution separation for precise and s...
Horizontal Electrophoresis System LHES-T2010
Horizontal Electrophoresis System has a buffer volume of 550 ml to deliver efficient, stable, and economical performance. It offers easy sample loading, reducing preparation time, and us...
Horizontal Electrophoresis System LHES-T2014
Horizontal Electrophoresis System supports four different gel dimensions with a buffer capacity of 650 mL. It has a transparent lid that enables real-time observation of the electrophore...
Horizontal Electrophoresis System (DUAL TANK) LHES-T2010
Horizontal Electrophoresis System ( ) is designed with three platinum electrodes in which one stand for negative terminal and rest two are positive terminal. It is composed with a tank, ...
Horizontal Electrophoresis System LHES-T2012
Horizontal Electrophoresis System is a high resolution horizontal unit with 3 different gel dimensions (W × throughput. Large volume buffer of 1800 ml ensures cooling effects and stable...
Horizontal Electrophoresis System LHES-T2013
Horizontal Electrophoresis System is a high resolution horizontal unit with gel dimension (W × throughput. Large volume buffer of 2000 ml ensures cooling effects and stable pH value for...
Horizontal Electrophoresis System LHES-T2010
Horizontal Electrophoresis System is a high resolution horizontal unit with gel dimension (W × throughput. Buffer volume of 150 ml provides cooling effect and stable pH during process. ...
Horizontal Electrophoresis System LHES-T2011
Horizontal Electrophoresis System offers a 10–30 sample capacity, ensuring flexibility for low- and high-throughput use. It delivers accurate band resolution with minimal samples, reduc...
Standards
Complies with the above standards, but is not limited to them.
Features
1. Horizontal electrophoresis system for separation of nucleic acids in agarose gels.
2. Submarine gel design with buffer tank for DNA and RNA fragment separation.
3. Suitable for molecular biology, genetics, clinical and research laboratories.
4. Includes gel tray, comb, buffer tank, lid with safety interlock and electrodes.
5. Supports DNA/RNA fragment analysis, genotyping, cloning verification and screening.
FAQ
Q1: What testing scenarios is this system mainly applied to?
A: The horizontal electrophoresis system is used in molecular biology, genetics, clinical diagnostics, forensic science, agricultural biotechnology and research laboratories to separate DNA and RNA fragments by size using agarose gel electrophoresis. It supports PCR product analysis and verification, DNA genotyping and fingerprinting, restriction enzyme digestion analysis, cloning and plasmid verification, nucleic acid quality assessment, forensic DNA analysis, and educational molecular biology demonstrations. The horizontal submarine gel design is the standard method for routine nucleic acid separation, providing fast, reliable size-based separation of DNA and RNA fragments. It is essential for molecular biology, genetics, clinical and research laboratories requiring nucleic acid separation for PCR verification, genotyping, cloning, DNA fingerprinting, quality control and research, where agarose gel electrophoresis is a fundamental technique for analyzing DNA and RNA fragment sizes, verifying amplification products, assessing nucleic acid quality, and supporting genetic analysis, molecular cloning, diagnostics and research in life science and biomedical applications.
Q2: What standards does the electrophoresis system comply with?
A: The horizontal electrophoresis system complies with relevant IEC 61010 laboratory electrical safety standards, including safety interlock features for high-voltage electrophoresis. Molecular biology applications follow standard laboratory protocols and good laboratory practice. The system uses standard agarose gel electrophoresis methods widely referenced in molecular biology protocols and publications. Users should follow appropriate safety procedures for ethidium bromide or alternative nucleic acid stains.
Q3: What is the warranty period for the whole machine and key components?
A: The whole electrophoresis system is covered by a 12-month warranty. The buffer tank, lid, electrodes and safety interlock are covered, while gel trays, combs and casting accessories are consumables or accessories. Damage from improper use, buffer corrosion or unauthorized repair is excluded.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires basic training in molecular biology techniques, agarose gel preparation and electrophoresis. We provide an operation manual and remote guidance. Proper gel casting, buffer preparation, sample loading, voltage setting and safety procedures are important for successful electrophoresis and safe operation.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular maintenance required? What is the recommended calibration cycle?
A: Electrode condition and buffer tank cleanliness checked regularly. Safety interlock function tested periodically. Gel trays and combs inspected for damage. Routine cleaning after each use to prevent buffer residue and contamination. No formal metrological calibration is typically required for the electrophoresis unit itself (power supply may require calibration).
Q7: Are supporting consumables universal?
A: Agarose, electrophoresis buffer, nucleic acid stains, molecular weight markers and pipette tips are available from multiple molecular biology suppliers. Gel trays and combs may be model-specific. Long-term consumable cost depends on experiment volume and reagent choices.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes gel size, number of combs, buffer capacity, electrode type and accessories. Additional gel trays, combs and casting accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: No complex installation is required beyond setup and power connection to a separate electrophoresis power supply. The package includes the electrophoresis system and instruction manual. On-site training service is available at additional cost for molecular biology laboratory setup.
Q10: What types of samples are compatible with this equipment?
A: The horizontal electrophoresis system separates DNA and RNA fragments in agarose gels within the gel size and voltage range. Protein samples require vertical (SDS-PAGE) systems instead. Very large DNA fragments may require pulsed-field electrophoresis. Samples should be properly prepared with loading buffer and appropriate nucleic acid stain. Users should select appropriate agarose concentration for the expected fragment sizes, use proper electrophoresis buffer (TAE or TBE), ensure complete gel coverage with buffer, load samples carefully, use appropriate voltage and run time, follow safety procedures for high-voltage operation and nucleic acid stains (particularly ethidium bromide which is mutagenic), and clean the apparatus thoroughly between runs to prevent cross-contamination.
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