Horizontal Electrophoresis System LHES-T2010

Introduction

Horizontal Electrophoresis System has a buffer volume of 550 ml to deliver efficient, stable, and economical performance. It offers easy sample loading, reducing preparation time, and user-friendly operation even for high-throughput workflows. The clear band visibility allows for precise observation and documentation of results. Its leak-proof gel tray safeguards against buffer spillage, helping maintain lab cleanliness. Our horizontal electrophoresis system has consistent voltage output for uniform electrophoretic separation and reproducible and accurate outcomes.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Horizontal Electrophoresis System is a laboratory apparatus that separates nucleic acids, primarily and , through an agarose gel under an electric field. Molecular biology, genomics, and forensic science commonly use it for fragment analysis.

Specifications

Gel Dimensions (W × L)	120 × 120 mm, 120 × 60 mm, 60 × 120 mm, 60 × 60 mm
Buffer Volume	550 ml
Sample Capacity	1 to 100
Power Supply	220 V | 50 Hz
Dimensions (L × W × H)	300 × 160 × 75 mm
Weight	1.1 kg

Features

Adjustable bottom footers

Gel tray drift prevention

Efficient cooling system

Stable pH maintenance

Dismountable quick electrodes

FAQs

1. What is a horizontal electrophoresis system used for?

horizontal electrophoresis system is a critical tool in molecular biology labs used to separate, analyze, and visualize nucleic acids namely and based on their size and electrical charge. When an electric field is applied across the gel, negatively charged fragments migrate toward the positive electrode, with smaller fragments moving faster than larger ones. This allows researchers to determine the size and integrity of the genetic material. Horizontal systems are most suitable for agarose gel electrophoresis and are ideal for processes like checking the success of cloning, verification, genetic fingerprinting, restriction digestion analysis, and plasmid isolation. systems are optimized to deliver consistent, high-resolution results, even for low-concentration samples.

2. What components are included in a horizontal electrophoresis system?

It provides a fully integrated setup that includes a gel casting tray (or trays of different sizes), a buffer tank with built-in platinum electrodes, combs for forming wells, a lid with safety interlock features, and often a gel-casting stand. High-performance models may also include bubble levels, gel rulers, and buffer recirculation options. The platinum wire electrodes are corrosion-resistant and ensure even current distribution across the gel. It designs each component to be modular and easy to assemble or clean, making the system adaptable for different experimental needs, from classroom education to advanced research labs.

3. How do I prepare a gel for horizontal electrophoresis system?

It recommends preparing an agarose gel by accurately weighing agarose powder and dissolving it in an appropriate buffer (like or ) through heating. Once the solution is clear and uniform, it is poured into a gel casting tray fitted with combs to form wells. After cooling and solidifying, the gel is carefully placed into the tank and fully submerged in buffer. Samples are then loaded into the wells along with a ladder or marker for comparison. gel trays are designed to resist warping and are marked for easy alignment, ensuring the gel sets evenly and without air bubbles, which can affect the accuracy of results.

4. What buffer should I use for horizontal electrophoresis system?

It supports the use of both (Tris-acetate-) and (Tris-borate-) buffers, depending on the nature of the analysis. is preferred when a faster migration rate is needed or when needs to be extracted from the gel for downstream applications like cloning. On the other hand, provides better resolution for smaller fragments and is preferred for longer runs due to its stronger buffering capacity. systems are engineered to maintain stable pH and current flow with either buffer, preventing overheating and ensuring consistent band patterns.

5. How do I ensure even migration of in the gel in horizontal electrophoresis system?

Some common mistakes when using an electrophoresis power supply include using incorrect voltage settings, improper gel loading, and connecting electrodes in the wrong orientation. Other errors include not using fresh buffer solutions, failing to check for damaged cables, and running the gel for too long or too short a time. These mistakes can lead to poor separation, sample loss, or even damage to the gel and equipment. power supplies come with clear display indicators, safety alarms, and user-friendly controls, helping researchers minimize errors and achieve consistent, high-quality results.