Introduction
A split beam UV-Vis spectrophotometer is an analytical instrument used to measure the absorption and the transmittance of the light by the samples over the ultraviolet and the visible wavelength regions, with the split beam optical design, and it is used in the chemistry, the biology, the clinical, the pharmaceutical, and the many other laboratories. The split beam UV-Vis spectrophotometer measures the absorbance of the light by the sample, with the light beam split into the sample beam and the reference beam by a beam splitter, and the ratio of the intensities is measured, which compensates for the fluctuations of the light source, providing the stable and the accurate measurements; the split beam UV-Vis spectrophotometer consists of a light source, a monochromator, a beam splitter, the sample and the reference compartments, a detector, and a control and a data analysis system, and it provides features such as the wavelength scanning and the absorbance measurement; the split beam UV-Vis spectrophotometer is used for the quantitative and the qualitative analysis, for the measurement of the concentration of the samples, for the quality control, for the research, and for many other applications, and it is an essential instrument for the UV-Vis analysis. Split beam UV-Vis spectrophotometers are used in the chemistry and the biology laboratories, in the clinical laboratories, in the pharmaceutical industry, in the food industry, and for many other applications. With their split beam design, split beam UV-Vis spectrophotometers are essential for the accurate absorbance measurements.
Split Beam UV-Vis Spectrophotometer T-US-D100
Standards
European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible
ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
Complies with the above standards, but is not limited to them.
Features
1. Split-beam UV-visible spectrophotometer for accurate absorbance measurement.
2. Split-beam optical design compensates for light source drift during measurement.
3. Wide wavelength range with scanning capability for spectral analysis.
4. Suitable for pharmaceutical, chemical, environmental and research laboratories.
5. Software supports spectral scanning, quantitation, kinetics and data management.
FAQ
Q1: What testing scenarios is this instrument mainly applied to?
A: The split-beam UV-visible spectrophotometer is used in pharmaceutical, chemical, environmental, food and research laboratories for qualitative and quantitative analysis by absorbance measurement in the ultraviolet and visible ranges. It supports drug content and impurity analysis, compound identification by spectral scanning, enzyme kinetics, nucleic acid and protein quantitation, water quality testing and method development. The split-beam design provides improved stability compared with single-beam instruments by splitting the light beam to compensate for source drift, making it suitable for longer scans and kinetic measurements where source stability affects result accuracy. It is ideal for laboratories requiring scanning capability and reliable performance for both routine analysis and research applications at a moderate cost point between single-beam and double-beam instruments.
Q2: What standards does the instrument comply with?
A: The instrument supports analytical methods referenced in USP, EP and ChP monographs, ASTM E169 for UV-Vis quantitative analysis, and relevant GB/T standards for spectrophotometry. The hardware complies with IEC 61010 laboratory safety standards. Wavelength and absorbance accuracy should be verified with certified reference materials.
Q3: What is the warranty period for the whole machine and key components?
A: The whole spectrophotometer is covered by a 12-month warranty. The optical system, detector, light source and controller are covered, while lamps, cuvettes and calibration standards are consumables excluded from warranty. Extended warranty is available.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained analysts familiar with spectrophotometric principles. We provide operation manuals, application notes and training. Routine fixed-wavelength measurement and spectral scanning can be performed by trained operators with software guidance.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Wavelength and absorbance calibration with certified reference materials is recommended every 6-12 months. Routine verification before each analytical batch is recommended for regulated work. Lamp replacement follows the manufacturer schedule.
Q7: Are supporting consumables universal?
A: Lamps, cuvettes and calibration standards are available from multiple qualified suppliers. Long-term consumable cost is moderate.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes wavelength range, resolution, sample compartment accessories and software modules. Additional accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Standard delivery includes the spectrophotometer and accessories with an operation manual. Remote commissioning guidance is provided. On-site installation and training can be arranged for laboratory setup projects.
Q10: What types of samples are compatible with this equipment?
A: The instrument analyzes liquid samples within the wavelength and absorbance range. Samples must be clear and homogeneous; turbid samples cause scattering errors. Highly corrosive samples require compatible cuvette material. Very high absorbance samples should be diluted to the linear range. Users should perform proper baseline correction and allow lamp warm-up before measurement for stable and accurate results.
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