Stainless Steel Bioreactor LSSB-T2010

Introduction

Stainless Steel Bioreactor has a total capacity of 30L with a working volume of 21L. This allows for efficient mixing, aeration, and enough headspace for proper gas exchange during fermentation. To control the temperature, the bioreactor uses a jacketed water bath that surrounds the tank. A circulating pump keeps the water moving through the jacket, ensuring even and consistent heat distribution. Our stainless-steel bioreactor is ideal for fermentation and bioproduction and is widely used in industries such as pharmaceuticals, food, energy, agriculture, and scientific research.

This bioreactor is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Stainless Steel Bioreactor is widely used in pharmaceuticals, food, energy, agriculture, and research for fermentation and bioproduction processes.

Specifications

Total Volume	30L
Working Volume	21L (70%)
Tank Material	SUS316L
Jacket Material	SUS304
Tank Shape Ratio	Diameter: Height = 1: 2.2 to 2.5 (good aeration)
Internal Surface Finish	Mirror polish, Ra < 0.4 µm
External Surface Finish	Matt or brushed
Sight Glass	Long, with wide viewing angle
Tank Lid Ports	Defoaming electrode, inoculation port, exhaust port, 4 feeding holes
Tank Bottom	Fermentation bottom valve, discharge valve, seed transfer pipeline; no dead corners; steam sterilizable
Interfaces	Temperature, pH, DO electrode interfaces, sampling valve
Design Pressure	Tank: 0.1 to 0.3 MPa; Jacket: -0.1 to 0.4 MPa
Sterilization	Online steam sterilization
Air Intake	1 (automatic)
Ventilation Method	Deep-layer, detachable annular air distributor
Air Control	(repeatability: 0.2% FS, stability <1%/year)
Max Ventilation	2 ; 0 to 350 L/min
Exhaust System Components	Exhaust gas condenser, separate sterilization/fume discharge, bypass
Exhaust Pipe	≥3° slope, liquid-free, bacteria-free
Pressure Measurement	Mechanical gauge + pressure sensor
Valve Control	Manual (automatic optional)
Exhaust System Display Range	0 to 0.4 MPa (±0.01 bar), control range: 0 to 0.25 MPa (±0.05 bar)
Exhaust System Controller Functions	DO-related control, analysis, batch report, alarms
Tank Sterilization Temp	100 to 130°C
Time	0 to 60 min
Control	High-quality diaphragm valve
Process Steps	Filter line sterilization, tank sterilization, sterile water system
Mixing System Stirring Type	Top-mounted mechanical
Shaft	Special alloy, precision balanced, stable, long-lasting
Mixing System Blade Design	1 quadriclinal + 2 six-flat blade (3-stage system)
Speed	30 to 418 rpm ±0.5%
Power	1 kW (customizable for higher speed)
Mixing System Controller Functions	DO cascade, curve analysis, data storage, sequence (10+ segments)
Heating Method	Electrically heated jacketed water bath
Heat Transfer	High efficiency with circulation
Sensor	Pt100, sterilizable, software calibration
Control Range	+5°C above cooling water to 50°C ±0.5°C
Display Range	0 150°C ±0.1°C
Controller Functions	control, sequence control (10+ stages)
pH Control Range	0.00 to 14.00 ±0.01; Control: 2.00 to 12.00 ±0.05
DO Control Range	0 to 150% ±3%
Control Method	Cascade with speed, feed, airflow
Foam Control Method	Defoaming electrode + peristaltic pump
Dimension	1650 × 1220 × 2130 mm
Packaging Dimension	1750 × 1350 × 2250 mm
Net Weight	480 Kg
Gross Weight	530 Kg

Features

Sight glass

Automatic air control

Exhaust gas condenser

Defoaming electrode

Alarm

Data logging

FAQs

1. What are the main features that make It Stainless Steel Bioreactors suitable for industrial fermentation and bioproduction?

It Stainless Steel Bioreactors are designed with both efficiency and precision in mind. These bioreactors are made using high-quality stainless-steel materials (typically SUS316L for the tank and SUS304 for the jacket), ensuring durability and corrosion resistance. They come equipped with features such as a sight glass for easy monitoring, automatic air control, defoaming electrodes, and advanced sensors for measuring pH, DO (dissolved oxygen), and temperature. The bioreactors also have a jacketed water bath with circulating pumps for even heat distribution, ensuring stable and consistent temperature control. These features make It bioreactors reliable tools for fermentation processes across a wide range of industries like pharmaceuticals, food production, agriculture, and scientific research.

2. How does It ensure proper temperature control in its stainless-steel bioreactors?

It uses a jacketed water bath system to manage temperature in its stainless-steel bioreactors. This jacket surrounds the main fermentation tank and is connected to a circulating pump that keeps water flowing continuously. This setup allows for even and consistent heat transfer throughout the tank. The system is electrically heated and includes Pt100 sensors, which are sterilizable and software-calibrated for precise temperature readings. The controller uses advanced (Proportional-Integral-Derivative) technology to maintain the desired temperature range, which can be set between +5°C above the cooling water and up to 50°C, with high accuracy (±0.5°C). This ensures optimal conditions for microbial or cell growth during fermentation.

3. What mixing system is used in It Stainless Steel Bioreactors and why is it effective?

It bioreactors use a top-mounted mechanical stirring system designed for stable and efficient mixing. The system includes a precision-balanced shaft made of a special alloy for long-lasting performance. It operates with a 3-stage blade design one quadriclinal blade and two six-flat blades—which helps create thorough mixing and enhances oxygen transfer within the fermentation medium. The speed is adjustable from 30 to 418 rpm with high accuracy (±0.5%), allowing users to fine-tune the mixing process based on the specific requirements of the biological culture. This robust and flexible mixing setup is essential for maintaining homogeneity and promoting healthy microbial or cell growth.

4. How does It support sterility and safety in its stainless-steel bioreactors?

Sterility is critical in fermentation processes, and It bioreactors are designed to meet this need. The bioreactors support online steam sterilization (also known as Sterilization In Place), allowing the tank, filters, and connected lines to be sterilized without dismantling. The internal surfaces are mirror-polished to a smoothness of Ra < 0.4 µm, reducing the risk of contamination from residues. The tank bottom is designed without dead corners and includes multiple sterilizable valves, such as a fermentation bottom valve and a discharge valve. Exhaust systems include condensers and separate sterilization/fume discharge units to handle waste gases safely. Combined with alarms, data logging, and automatic controls, these features ensure both sterility and operator safety.

5. In what industries can It Stainless Steel Bioreactors be used, and what processes do they support?

It Stainless Steel Bioreactors are highly versatile and find applications across several major industries. In the pharmaceutical industry, they are used for producing antibiotics, enzymes, vaccines, and recombinant proteins. In the food and beverage sector, they assist in the fermentation of products like yogurt, alcohol, and probiotics. In agriculture, they help in the production of biofertilizers and biopesticides. They are also useful in the energy sector for the development of biofuels and in scientific research for studying cell behavior and metabolic processes. Thanks to their precision, control systems, and scalability, It bioreactors support both small-scale R&D and large-scale industrial bioproduction.