Vertical Electrophoresis System LVES-T2012

Introduction

Vertical Electrophoresis System has a gel thickness of 1.0 mm, ensuring uniform electrophoresis results with consistent band resolution. It features low buffer consumption, optimizes reagent usage, and reduces operational costs. The system provides enhanced sample loading precision, minimizing errors and improving the accuracy of sample placement. Its heat dissipation efficiency prevents overheating, maintaining stable conditions for effective gel separation. Our system has minimized the risk of sample leakage, ensuring reliable and contamination-free results.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Vertical Electrophoresis System  is used to separate proteins or nucleic acids based on their size and charge through a vertical gel matrix. It is used in molecular biology, and clinical research for protein analysis, / separation, and genetic studies.

Specifications

Glass Plate Dimensions	216 × 110 mm
Gel Dimensions	186 × 95 mm
Gel Thickness	1.0 mm
Number of Gels	1 to 2 pcs
Maximum Buffer Volume	1800 ml
Sample Throughput	25 to 104
Dimension (L × W × H)	290 × 170 × 155 mm
Weight	3.2 kg

Features

Leak-Proof Gel Casting

Uniform Band Resolution

Versatile Gel Thickness Options

Easy Lid Opening Mechanism

Efficient Buffer Circulation System

FAQs

1. What types of gels can be used in Vertical Electrophoresis System?

Vertical Electrophoresis System is compatible with both polyacrylamide () and agarose gels, depending on the application. Polyacrylamide gels are typically used for protein separation, while agarose gels are more commonly used for and analysis. It provides detailed protocols and recommendations for preparing and loading different gel types, ensuring efficient separation and consistent results.

2. Can different running buffers be used with Vertical Electrophoresis System?

Yes, Vertical Electrophoresis System supports a variety of running buffers, such as Tris-Glycine, Tris-Tricine, and /, depending on the type of gel and the target molecules. Using the appropriate buffer ensures optimal conductivity and efficient migration of biomolecules. system design minimizes buffer leakage and maintains stable buffer conditions throughout the run, ensuring high-quality separation.

3. Is it possible to cast gels directly in Vertical Electrophoresis System?

Yes, Vertical Electrophoresis System is designed for in-situ gel casting, allowing researchers to cast gels directly within the unit. The system includes leak-proof gel casting clamps and spacers, ensuring uniform thickness and reducing the risk of gel deformation. system provides consistent polymerization conditions, preventing air bubble formation and ensuring a smooth gel surface. This feature eliminates the need for separate gel casting equipment, streamlining the workflow, and reducing handling errors.

4. Does Vertical Electrophoresis System have safety features to prevent overheating?

Vertical Electrophoresis System is equipped with advanced safety features to protect both the equipment and the user from overheating and electrical hazards. The system includes automatic shutdown mechanisms that activate if abnormal voltage fluctuations or overheating are detected during the run. Additionally, It has incorporated heat dissipation mechanisms into the system’s design, which maintain a stable running temperature, preventing gel distortion and sample degradation. These safety features enhance the longevity of the equipment while ensuring reliable performance over extended periods.

5. Can Vertical Electrophoresis System be used with different power supplies?

Yes, Vertical Electrophoresis System is compatible with a wide range of power supplies, providing flexibility in voltage and current settings based on experimental requirements. It recommends using power supplies with adjustable voltage and current limits to achieve optimal results. The system allows users to set the desired voltage for different applications, ranging from low-voltage runs for nucleic acids to higher voltages for protein separations. power supply units ensure steady and consistent power delivery, minimizing fluctuations and ensuring reproducible electrophoresis results.

6. Is it possible to run gradient gels in Vertical Electrophoresis System?

Yes, Vertical Electrophoresis System supports the use of gradient gels, which offer superior separation for molecules with similar molecular weights. Gradient gels create a gradual change in pore size across the gel, allowing for more effective separation of complex protein or nucleic acid mixtures. It provides protocols and guidelines for preparing and running gradient gels, ensuring accurate separation and high-resolution band patterns. This capability makes the system ideal for applications requiring high sensitivity and precision, such as protein characterization and differential expression analysis.

7. How should gels be stained and visualized after running on Vertical Electrophoresis System?

After completing electrophoresis, gels run using Vertical Electrophoresis System can be stained using a variety of staining methods to visualize separated biomolecules. For protein gels, commonly used stains include Coomassie Brilliant Blue, silver staining, and fluorescent dyes. For nucleic acid gels, ethidium bromide (EtBr), Green, and GelRed are often used. It provides detailed guidelines for staining protocols and recommends using high-sensitivity stains to enhance visualization. The system ensures clear band resolution, making downstream analysis more accurate and reliable.