Single Beam UV-Visible Spectrophotometer LUS-T312

Introduction

Single Beam UV-Visible Spectrophotometer features a wavelength resolution of 0.1 nm for analyzing complex samples or subtle spectral shifts with high precision. The spectrophotometer has tungsten and deuterium lamps, ensuring complete spectral coverage. It includes holographic diffraction grating for uniform spectral efficiency across the wavelength range. Our single beam UV-visible spectrophotometer offers three adjustable scanning speeds to balance scan duration and data quality.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Single Beam UV-Visible Spectrophotometer  passes monochromatic light through a sample to determine chemical characteristics. It is applied in biochemical analysis, environmental monitoring and pharmaceutical industries in quality control.

Specifications

Spectral Bandwidth	4nm (optional 2nm)
Wavelength Range	320 to 1100 nm
Optical System	Single beam path, holographic diffraction grating
Wavelength Accuracy	± 0.8 nm
Wavelength Repeatability	0.2 nm or less
Wavelength Setting	Automatic Wavelength Setting
Wavelength Resolution	0.1 nm
Luminosity Range	0 to 200% T, -0.3 to 3A, 0 to 9999 C, 0 to 9999 F
Photometric Accuracy	±0.002A (0 to 0.5A), ±0.004A (0.5 to1A), ±0.3%T (0 to100%T)
Photometric Repeatability	≤ 0.001A (0 to 0.5A), ≤0.002A (0.5 to 1A), ≤0.2%T (0 to 100%T)
Stray Light	≤0.1%T (220/360nm)
Baseline Drift	≤0.002A/ h (500nm,0A)
Noise	± 0.2%T (0%T line) ±0.4%T (100%T line) ±0.001A (500nm)
Baseline Flatness	±0.003A
Scan Speed	Three adjustable speeds: high, medium and low
Detector	Imported silicon photodiode
Light Source	Imported long-life tungsten and deuterium lamps
Display	3-inch 128× 64 dot matrix display
Keyboard	Tap film keys
Data Interface	Port
Printout	Microprinter, PC (Optional)
Power Supply	AC 902 to50V, 50/60Hz
Overall Dimensions	430 ×320 ×195mm
Weight	10 kg

Features

Wide Wavelength Range

Standard Curve Display

Silicon Photodiode Sensors

Dual Lamp Sources

Adjustable Scan Speed

FAQs

1. How does the Single Beam UV-Visible Spectrophotometer F12 reduce stray light effects at high absorbance?

Single Beam UV-Visible Spectrophotometer F12 Single Beam UV-Visible Spectrophotometer minimizes stray light—unwanted wavelengths that can distort measurements at absorbance over ~2?AU—by using high-grade holographic gratings and tight optical seals, ensuring more accurate results in high-concentration assays. Its stray light performance is validated using cutoff filters like sodium nitrite or potassium chloride, and should meet or exceed pharmacopeial standards for dynamic range. Proper external checks and maintenance ensure this capability remains intact over its service life.

2. How does the Single Beam UV-Visible Spectrophotometer F12 maintain wavelength accuracy and resolution over time?

Single Beam UV-Visible Spectrophotometer incorporates automated wavelength calibration using certified standards like mercury or holmium filters, allowing users to self-verify and adjust peak positioning within ±0.1?nm accuracy. Its high-resolution holographic diffraction grating further ensures crisp spectral resolution so closely spaced peaks remain identifiable. Routine performance checks help catch minimal drift early, maintaining consistent analytical precision for critical quantitative applications.

3. How can I troubleshoot ghost peaks or spectral anomalies on the Single Beam UV-Visible Spectrophotometer F12?

Single Beam UV-Visible Spectrophotometer may occasionally display ghost peaks due to internal reflections or diffraction from mirrors, gratings, or particles within the optical path. To combat this, clean and inspect all optics, ensure the monochromator slits are free from debris, and verify that internal surfaces remain well-aligned. Regular stray-light tests using cutoff filters can expose specific sources of unwanted signals, enabling precise corrective action.

4. What causes non-linear Beer Lambert behavior in the Single Beam UV-Visible Spectrophotometer F12, and how to correct it?

It supplies advanced Single Beam UV-Visible Spectrophotometer , deviations from linear absorbance arise when sample absorbance exceeds ~1?AU due to stray light, saturation, or chemical interactions like aggregation or refractive index shifts. To maintain Beer Lambert compliance, dilute samples, use shorter pathlength cuvettes, filter turbid solutions, and validate linearity with calibration curves. These strategies preserve quantitation integrity across concentration ranges.

5. How does the F12 manage lamp-switch artifacts in UV–Vis scans?

Single Beam UV-Visible Spectrophotometer transitions between deuterium and tungsten lamps around 320–360?nm; this switch can introduce artifacts if not synchronized well. To reduce crossover noise, the system delays data acquisition during lamp transitions, employs precise lamp alignment routines, and allows users to adjust the switch threshold in software. Periodic maintenance ensures smooth lamp toggling and continuity of spectra across the UV–Vis boundary.