Introduction

Single Beam UV-Visible spectrophotometer offers a spectral bandwidth of 4 nm, enabling clear peak resolution while maintaining a high signal-to-noise ratio. The system features a dot-matrix readouts with simple graphic visuals. It includes a blank-sample correction mode for eliminating background absorbance from cuvettes and solvents. Our single-beam UV-visible spectrophotometer features low-stray light performance, ensuring high photometric accuracy.

This analytical equipment is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Single Beam UV-Visible Spectrophotometer  used for quantitative analysis of compound concentration via the Beer-Lambert Law. It plays a key role in chemistry, biochemistry, pharmaceutical, clinical, environmental, water testing, food and beverage sector.

Specifications

Spectral Bandwidth 4nm
Wavelength Range 190 to 1020 nm
Optical System Single beam path, holographic diffraction grating
Wavelength Accuracy ± 2.0 nm
Wavelength Repeatability 0.5 nm or less
Wavelength Setting Automatic Wavelength Setting
Wavelength Resolution 0.1 nm
Luminosity Range 0 to 200% T, -0.3 to 3A, 0 to 9999 C, 0 to 9999 F
Photometric Accuracy ±0.002A (0 to 0.5A), ±0.004A (0.5 to1A), ±0.3%T (0 to100%T)
Photometric Repeatability ≤ 0.001A (0 to 0.5A), ≤0.002A (0.5 to 1A), ≤0.2%T (0 to 100%T)
Stray Light ≤0.1%T (220/360nm)
Baseline Drift ≤0.002A/h (500nm,0A)
Noise ± 0.2%T (0%T line)
±0.4%T (100%T line)
±0.001A (500nm)
Baseline Flatness ±0.003A
Scan Speed Three adjustable speeds: high, medium, and low
Detector Imported silicon photodiode
Light Source Imported long-life tungsten and deuterium lamps
Display 3-inch 128× 64 dot matrix display
Keyboard Tap film keys
Data Interface Port
Printout Microprinter, PC (Optional)
Power Supply AC 90 to 250V, 50 /60Hz
Overall Dimensions 430 ×320 ×195mm
Weight 12 kg

Features

Multi-Wavelength Testing

Narrow Spectral Bandwidth 

Automatic Wavelength Calibration

UV‑Vis Light Switching 

Dark‑Current Correction 

FAQs

1. How can user compensate for baseline drift during long-wavelength scans in Single Beam UV-Visible Spectrophotometer ?

It supplies premium Single Beam UV-Visible Spectrophotometer . Baseline drift often stems from light source instability or temperature changes, particularly in extended UV–Vis scans. To compensate, warm up the instrument for at least 20–30 minutes, perform blank measurements regularly, and keep the instrument in a temperature-controlled environment. Using baseline subtraction against a reference sample and periodically re-zeroing throughout long scans also maintains a flat baseline. Sealing cuvette holder lids tightly prevents stray light intrusion, further reducing drift. Cleaning dust and moisture from the optics as part of routine maintenance also supports stability.

2. Why do user see noise or peaks around lamp crossover wavelengths in Single Beam UV-Visible Spectrophotometer ?

It supplies premium Single Beam UV-Visible Spectrophotometer . Noise or ghost peaks around the UV–Vis lamp switch point (typically 320–360?nm) result from mechanical switching delays or misalignment between deuterium and tungsten lamps. Such artifacts appear during spectral scans when the instrument transitions lamps without pausing data capture. Regular alignment checks and ensuring clean optical paths reduce switching artifacts. Some models allow adjusting or smoothing the lamp crossover point during operation. If persistent, recalibrate using certified filters and inspect the lamp-switching mechanism for wear or contamination.

3. How do I detect stray light and why does it matter in Single Beam UV-Visible Spectrophotometer ?

Single Beam UV-Visible Spectrophotometer have advanced features. Stray light, defined as unwanted wavelengths reaching the detector, can lead to inaccurate absorbance readings. You can detect it by placing a cutoff filter or holmium oxide solution and scanning below its cutoff wavelength—non-zero transmittance indicates stray light. Minimizing stray light requires using narrow slit widths, clean optics, and proper alignment. Instruments with holographic diffraction gratings also reduce stray light by suppressing ghost orders. For critical applications, periodically test stray light levels and clean optical surfaces.

4. What causes nonlinear absorbance at high analyte concentrations in Single Beam UV-Visible Spectrophotometer ?

Single Beam UV-Visible Spectrophotometer have advanced features. Nonlinear absorbance (deviation from Beer–Lambert law) occurs at high concentrations due to self-association, refractive index effects, or stray light interference. To address this, dilute your sample or reduce path length to bring absorbance below ~1?AU. For turbid or colloidal samples, filtration or centrifugation minimizes scattering effects. Recording spectra at multiple concentrations and using calibration curves under real sample conditions also helps correct for nonlinear behaviour and improve accuracy.

5. How do I identify and fix dark-current errors in Single Beam UV-Visible Spectrophotometer have advanced features?

It supplies premium Single Beam UV-Visible Spectrophotometer . Dark-current errors indicate the detector is receiving light when it should be blocked (or the detector itself is faulty). To diagnose, ensure the beam shutter/lid properly closes during zeroing; any light leak will trigger the error. After confirming darkness, allow full lamp warm-up and inspect/re-align the photodetector and light path. If the issue persists, check for worn photodiodes or PMTs and replace the detector or amplify circuitry. Maintaining cleanliness and serviceable shutters prevents recurrence.

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