2D Gel Electrophoresis System LDGES-T2010

Introduction

2D Gel Electrophoresis System first-dimension tube gel electrophoresis system features 120 mm and is compatible with various sample sizes. It has an advanced gel imaging system, providing high-resolution visualization for precise analysis. The system supports rapid electrophoresis processing, reducing experiment time while maintaining accuracy. Its minimal buffer consumption optimizes reagent usage, making it cost-effective. Our system has consistent cooling efficiency, maintains stable operating conditions, and ensures reliable and reproducible results.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

2D Gel Electrophoresis System is a specialized technique to separate complex protein mixtures based on molecular weight and isoelectric point. It is widely used in proteomics research, and functional genomics to analyze protein expression and identify biomarkers.

Specifications

Length of 1st D Tube gel Electrophoresis	120 mm
Gel Diameter of 1st D Tube Gel Electrophoresis	1.0 mm, 2.00 mm
Gel Dimensions of 2nd Vertical Electrophoresis (W × L)	143 × 139 mm
Gel Thickness of 2nd Vertical Electrophoresis	1.0 mm, 2.00 mm
Number of Gels	2 pcs
Maximum Buffer Volume	2500 ml
Sample Throughput	17 to 58
Dimension (L × W × H)	460 × 230 × 400 mm
Weight	13.2 kg

Features

Independent electrophoresis tanks

Efficient cooling water system

Easy-open lid design

Uniform sample separation 

Precise electrophoresis control

FAQs

1. How is the gel prepared for 2D Gel Electrophoresis?

In 2D Gel Electrophoresis, gel preparation involves two main steps: preparing the isoelectric focusing () gel for the first dimension and the - gel for the second dimension. The gel is used to separate proteins by their isoelectric point, and it is typically prepared using polyacrylamide gels with a pH gradient. After the first dimension, the gel is laid on top of the - gel, and the proteins are separated by their molecular weight in the second dimension. 2D Gel Electrophoresis Systems are designed to simplify and optimize the gel preparation process, ensuring reproducible results and efficient protein separation.

2. What is the benefit of using 2D Gel Electrophoresis over one-dimensional methods?

The primary benefit of 2D Gel Electrophoresis over one-dimensional methods is the higher resolution and the ability to separate proteins based on two different parameters (isoelectric point and molecular weight). This allows for the separation of thousands of proteins in a single experiment, offering significantly better proteome coverage. Additionally, it enables the analysis of complex biological samples where many proteins are present in similar molecular weight ranges. 2D Gel Electrophoresis Systems provide exceptional resolution and sensitivity, ensuring researchers can analyze complex protein mixtures with precision.

3. How long does the 2D Gel Electrophoresis process take?

The 2D Gel Electrophoresis process typically takes several hours to a full day, depending on the complexity of the sample and the specific protocols being used. The first dimension (isoelectric focusing) can take several hours, depending on the gradient used and the type of proteins being separated. The second dimension (-) usually takes a few hours. After running the gels, additional steps such as staining and imaging can also take additional time. 2D Gel Electrophoresis Systems are designed for efficient, high-throughput workflows, allowing for rapid processing while maintaining high-quality results.

4. Can 2D Gel Electrophoresis be used for quantitative analysis?

Yes, 2D Gel Electrophoresis can be used for quantitative protein analysis. After protein separation, the intensity of the spots or bands on the gel can be quantified using imaging systems that measure the light absorption or fluorescence of stained proteins. This allows for the comparison of protein expression levels across different conditions or samples. systems are equipped with advanced image analysis software that can perform spot detection, quantification, and normalization, providing accurate and reproducible quantitative data from 2D gels.

5. What types of stains can be used in 2D Gel Electrophoresis?

Several types of stains can be used in 2D Gel Electrophoresis, including Coomassie Brilliant Blue, silver stain, and fluorescent dyes. Coomassie Brilliant Blue is commonly used for general protein detection due to its sensitivity and ease of use. Silver stain is more sensitive and can detect proteins at lower concentrations, but it is more labor-intensive. Fluorescent dyes, such as Ruby, offer even higher sensitivity and can be used for multiplexing in mass spectrometry applications. 2D Gel Electrophoresis Systems are compatible with all these staining techniques, providing flexibility based on the researcher’s needs.

6. What is the role of the first dimension () in 2D Gel Electrophoresis?

The first dimension, Isoelectric Focusing (), is crucial because it separates proteins based on their isoelectric point (pI). Each protein has a unique pI at which it is electrically neutral, and takes advantage of this by applying a pH gradient to the gel. As the sample is subjected to an electric field, proteins migrate until they reach the point where their net charge is zero (their pI). This step allows for highly efficient separation of proteins, especially when dealing with complex samples. systems optimize by providing precise control over the pH gradient and electrical field, ensuring high-resolution protein separation.