Vertical Electrophoresis System LVES-T2017

Introduction

Vertical Electrophoresis System has gel dimensions of 82 × 88 mm, providing a suitable platform for various electrophoresis applications. It features a rapid gel casting process that minimizes preparation time and enhances efficiency. The system ensures accurate band separation, delivering precise and reliable results. It has adjustable running parameters users optimize conditions for different sample types. Our system has a shockproof lid design that enhances user safety by preventing accidental exposure during operation.

This electrophoresis system is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

Vertical Electrophoresis System  is used to separate proteins or nucleic acids based on their size and charge through a vertical gel matrix. It is used in molecular biology, and clinical research for protein analysis, / separation, and genetic studies.

Specifications

Glass Plate Dimensions	100 × 100 mm
Gel Dimensions	82 × 88 mm
Gel Thickness	0.75 mm, 1.0 mm, 1.5 mm
Number of Gels	1 to 2 pcs
Maximum Buffer Volume	750 ml
Sample Throughput	11 to 30
Dimension (L × W × H)	150 × 120 × 115 mm
Weight	1.5 kg

Features

Precise Voltage Control

Stable Gel Migration

Seamless Lid Locking

Compact Laboratory Footprint

Easy Post-Run Cleanup

FAQs

1. How does gel thickness affect the resolution in Vertical Electrophoresis System?

Gel thickness is a critical parameter that directly influences the resolution and separation efficiency in electrophoresis. Thicker gels allow for higher sample capacity but may slow down the migration and reduce band sharpness due to increased resistance. On the other hand, thinner gels provide better resolution by allowing faster migration and sharper band separation but may limit the sample volume that can be loaded. Vertical Electrophoresis System is designed to support multiple gel thickness options, enabling researchers to select the ideal thickness depending on the molecular size and type of analysis.

2. Can the gel plates and combs be reused in Vertical Electrophoresis System?

Yes, the gel plates and combs in Vertical Electrophoresis System are built from high-quality, durable materials that allow for multiple uses with appropriate care. To ensure longevity and consistent performance, It recommends cleaning the plates and combs immediately after each run using mild detergent and deionized water. Any residual buffer, gel fragments, or contaminants should be carefully removed to prevent interference with subsequent runs. It advises against using harsh chemicals that may damage the surfaces and recommends periodically inspecting the plates and combs for cracks or warping.

3. Is it necessary to equilibrate gels before running them in Vertical Electrophoresis System?

Yes, gel equilibration is an important step that ensures uniform migration and prevents inconsistencies in separation. Before running the gel, It recommends equilibrating it in the running buffer for at least 15–30 minutes to balance the ionic environment and remove any unpolymerized acrylamide or polymer residues. This process also stabilizes the gel matrix and helps to maintain an even electric field across the gel. Skipping equilibration can lead to uneven band migration and poor resolution. detailed operating manual highlights the importance of proper equilibration procedures to achieve optimal and reproducible results.

4. How does Vertical Electrophoresis System prevent gel overheating during extended runs?

Vertical Electrophoresis System includes built-in heat dissipation mechanisms that protect the gel from overheating during prolonged electrophoresis runs. Heat buildup can affect sample integrity, leading to distorted band patterns or smearing. system promotes buffer recirculation to maintain consistent temperature levels. For high-voltage applications or extended runs, It recommends using an external cooling system or reducing the voltage slightly to prevent excessive heat generation.

5. How can the buffer volume be adjusted to accommodate different gel sizes in Vertical Electrophoresis System?

The buffer volume in Vertical Electrophoresis System should be adjusted according to the gel size to ensure uniform migration and prevent buffer depletion during the run. Larger gels may require a higher buffer volume to maintain consistent ionic strength, while smaller gels can run efficiently with less buffer. It recommends filling the upper and lower reservoirs adequately to ensure that the electrodes remain fully submerged and the electric field remains stable. Insufficient buffer levels can result in uneven migration, while excessive buffer may dilute the ionic strength, impacting resolution. user manual provides buffer volume recommendations tailored for different gel sizes and applications.

6. How can air bubbles be minimized in gel wells while using Vertical Electrophoresis System?

Air bubbles trapped in the wells or between the plates can interfere with sample migration and lead to distorted or irregular bands. To minimize bubble formation, It recommends degassing the gel solution before pouring to remove dissolved gases that may cause bubbles during polymerization. Additionally, inserting the combs gently at a slight angle while pouring the gel helps prevent air entrapment. precision-molded combs and plates further reduce the risk of air bubbles by ensuring uniform gel formation. Careful handling and proper preparation techniques ensure smooth sample loading and consistent results.

7. How does Vertical Electrophoresis System ensure proper alignment of gel plates?

Proper alignment of gel plates is essential to prevent buffer leakage and ensure an even electric field during electrophoresis. Vertical Electrophoresis System features precision-molded spacers and clamps that hold the plates securely in place, preventing misalignment. During assembly, It advises ensuring that the plates are parallel and properly tightened to avoid any gaps that could result in buffer leakage. Misalignment can lead to uneven migration and inconsistent band patterns, but detailed instructions guide users through the correct plate assembly process to achieve optimal results.