Water Jacketed CO2 Incubator LWJI-T2014

Introduction

Water Jacketed CO₂ Incubator offers 0 to 20 % CO₂ range, water jacketed heating method and polished stainless steel chamber having 170 L capacity. Chamber is incorporated with filter to maintain aseptic condition, prevent culture cross contamination, and to effectively filter out the outside bacteria & dust particles. Features microbial efficient filter with diameter <0.3 um at CO₂ inlet with 99.99% efficiency provides effective filtration (removal of bacteria and dust) and supplies pure CO₂ into the incubator. Designed with imported (IR) Infrared sensor to keep CO₂ concentration inside the chamber stable and uniform as door is open frequently. It provides a stable and controlled environment for cell culture, microbial growth, and other biological experiments. The precise temperature and humidity control ensure consistent experimental conditions and reliable results.

Applications

Water jacketed CO2 incubators are widely used for a broad range of applications that includes tissue engineering, In vitro fertilization, neuroscience, cancer research, stem cell research, regenerative medicine and mammalian cell research.

Specifications

Heating method	Water Jacketed
Chamber volume	170 L
CO2 range	0 to 20 %
CO2 control resolution	± 0.1% (IR Sensor)
CO2 recovery	(Door open 30 s, recovery to 5%) ≤ 3 min
Temperature range	RT + 5 to 50°C
Ambient temperature	5 to 30°C
Temperature stability	± 0.1°C
Temperature recovery	(Door open 30 s, recovery to 37℃ ) ≤ 8 min
Humidity method	Natural vaporization > 95 %
No. of shelves	3 pcs
Sterilization method	UV lamp sterilization
Interior dimension	530×460×720 mm
Exterior dimension	684×700×960 mm
Power consumption	700 W
Power supply	AC 220 V, 50 Hz
Packaging dimension	820×810×1230 mm
Packaging weight	125 kg (1 )

Features

Water Jacketed Type Heating Method

Microprocessor controlled system

Semicircular arcs at corners for easy cleaning

3 pcs of adjustable shelves inside the chamber

Faster CO₂ concentration restoration speed

Built-in UV germicidal lamp in the rear of top of the box of effective sterilization

Door temperature controller prevents dewfall on glass door of incubator effectively

Pt100 Temperature sensor to ensure accurate temperature

Can adjust heating power in real time

Touch screen controller display (shows real time curve with curve summary function)

Can run continuously up to 72 hours

filter: Cleanliness level 100 (air quality inside chamber) is recovered on 5 minutes door closing

Audible and visible temperature-limiting alarm system to ensure safe running of the experiments

Auto-controller of fan speed to prevent damage to the samples

Alarm function for CO₂ over concentration and concentration difference, door open time, UV lamp working status

FAQs

1. How to calibrate the temperature and humidity controls in the laboratory incubator?

Calibration should be performed by qualified personnel using calibrated temperature and humidity sensors: 1) Place the calibrated sensors at different locations inside the incubator chamber to ensure uniform distribution; 2) Set the incubator to the desired temperature and humidity levels and allow it to stabilize for at least 24 hours; 3) Compare the readings from the incubator's display with the calibrated sensors; 4) Adjust the controls according to the manufacturer's instructions if discrepancies are found; 5) Document the calibration results and schedule regular recalibration (every 6-12 months).

2. What is the optimal way to arrange samples inside the incubator for uniform conditions?

To ensure uniform conditions: 1) Avoid overcrowding the incubator, leaving sufficient space between samples for air circulation; 2) Place samples away from the chamber walls, heating/cooling elements, and door to prevent temperature fluctuations; 3) Use shelves or racks that allow air to circulate freely around all samples; 4) Avoid placing large or heat-generating equipment inside the incubator; 5) Rotate samples periodically if long-term incubation is required to account for any minor temperature variations.

3. Can the incubator be used for anaerobic or CO2-enriched environments?

Specialized incubators (anaerobic incubators, CO2 incubators) are designed for these environments. Standard incubators may not maintain the required gas concentrations or oxygen-free conditions. For anaerobic applications, use an incubator equipped with a gas control system that maintains oxygen levels below 1% and may include catalysts to remove oxygen. For CO2-enriched environments, use a CO2 incubator that maintains precise CO2 concentrations (typically 5-10%) and humidity levels, essential for cell culture applications.

4. How to clean and disinfect the incubator to prevent contamination?

Regular cleaning and disinfection steps: 1) Turn off the incubator and allow it to cool down to room temperature; 2) Remove all samples and shelves; 3) Wipe the interior surfaces with a mild detergent solution, followed by a disinfectant compatible with the incubator materials (such as 70% ethanol); 4) For stubborn contamination, use a specialized laboratory disinfectant according to the manufacturer's instructions; 5) Clean the shelves and any accessories separately; 6) Allow all surfaces to dry completely before reassembling and restarting the incubator; 7) Perform this procedure monthly or more frequently if contamination is suspected.

5. What should be done if the incubator temperature fluctuates significantly?

If temperature fluctuations occur: 1) Check if the incubator door is properly closed and sealed; 2) Ensure the incubator is not placed near heat sources, air conditioning vents, or drafty areas; 3) Verify that the incubator is not overloaded, which can restrict air circulation; 4) Check the air filter (if equipped) and replace it if dirty; 5) Inspect the heating and cooling elements for proper operation; 6) If the problem persists, contact technical support for further diagnosis and repair, as it may indicate a malfunction in the temperature control system.