Electroporator LGE-T2010

Introduction

Electroporator is a compact, modular electroporation system, where electrical pulse is used to create temporary pores in cell membranes through which substances like nucleic acids can pass into cells. The instrument is of a complete set with man-computer dialog interface. Once optimum electroporation conditions achieved, this Gene Electroporator transfects a large number of cells in a short time. The detailed setting range of capacitance and resistance makes the electro-perforation experiment of cells more selective under the related conditions. This electroporator is designed to meet the rigorous requirements of modern laboratory operations, ensuring high precision, reliability, and ease of use for various experimental applications.

Applications

It finds application in the electro-perforation of microorganism cells of animals and plants and is also used in cell hybridization, electroporation and silencing across molecular biology, genetic engineering and other research fields.

Specifications

High Output voltage	400 to 2500 V
Low output voltage	100 to 450 V
High voltage capacitor	1, 5, 6, 25, 30, 31 µF
Low voltage capacitor	100 µF，125 µF，150 µF …1675 µF, one grades of 25 µF
Resistance	50 Ω, 100 Ω, 150 Ω, 1600 Ω, increment total 30 grades
Operation system	Microcomputer control
Output waveform	With RC time constant of the exponential decay of wave
Dimension	368 × 316 × 229 mm
Weight	10.5 kg
Packing dimension	480 × 420 × 280 mm

Features

Exponential wave form output

Easy and rapid transfection of large number of cells in short time

Intelligent network system for on-site measurement and controlling parameters close to network

Networking of gene importer system; Two-way communication functions

Microprocessor controlled technology

User-friendly, simple operations

FAQs

1. What are the key factors to consider when selecting this electroporator for laboratory use?

When selecting this electroporator, key factors include: 1) The instrument's precision and accuracy to meet experimental requirements; 2) Compatibility with the types of samples and applications you will be working with; 3) Ease of use and maintenance requirements; 4) Safety features to protect operators and samples; 5) Calibration and certification requirements; 6) Cost-effectiveness and long-term reliability; 7) Technical support and service availability from the supplier. Evaluating these factors ensures that the instrument meets your laboratory's specific needs.

2. How often should this electroporator be calibrated to ensure accurate results?

It is recommended to calibrate this electroporator at least once every 6-12 months under normal usage conditions. More frequent calibration (every 3-6 months) may be necessary if: 1) The instrument is used continuously for extended periods; 2) It is exposed to harsh environmental conditions (temperature fluctuations, humidity, dust); 3) It is used for critical applications requiring high precision; 4) The instrument has been moved or subjected to physical shock; 5) Calibration checks indicate significant deviations from standard values. Always follow the manufacturer's calibration guidelines.

3. What are the common maintenance procedures required for this electroporator?

Common maintenance procedures include: 1) Regular cleaning of the instrument's exterior and sample contact surfaces to prevent contamination and ensure proper operation; 2) Inspection of moving parts (if applicable) for wear and lubrication as recommended by the manufacturer; 3) Replacement of consumable parts (filters, bulbs, reagents) according to usage guidelines; 4) Verification of performance parameters (temperature, pressure, voltage) regularly; 5) Storage of the instrument in a clean, dry environment when not in use; 6) Keeping a maintenance log to track service activities and identify potential issues early; 7) Following the manufacturer's specific maintenance schedule for optimal performance and longevity.

4. Can this electroporator be used with different types of samples, and are there any compatibility considerations?

Yes, this electroporator can be used with a variety of sample types, but compatibility considerations are important: 1) Check if the instrument's materials are compatible with the chemical composition of your samples (avoid corrosive or reactive substances that may damage the instrument); 2) Ensure that the sample volume and concentration are within the instrument's recommended range; 3) For biological samples, consider sterility requirements and potential cross-contamination risks; 4) For volatile or hazardous samples, ensure proper ventilation and safety precautions are in place; 5) Consult the manufacturer's documentation for specific sample compatibility guidelines, and perform compatibility tests with new sample types before regular use.

5. What should be done if the electroporator malfunctions or produces inconsistent results?

If malfunctions or inconsistent results occur: 1) First, check the instrument's user manual for troubleshooting guidelines specific to the issue; 2) Verify that the instrument is properly calibrated and within its calibration period; 3) Inspect for obvious issues such as loose connections, damaged parts, or incorrect settings; 4) Clean the instrument thoroughly to remove any contamination that may affect results; 5) Repeat the experiment with a known standard sample to determine if the issue is with the instrument or the sample; 6) If the problem persists, discontinue use of the instrument to prevent further damage or inaccurate results; 7) Contact technical support or a qualified service technician for diagnosis and repair, providing details of the issue and any troubleshooting steps already performed.