Introduction

Gradient Thermal Cycler offers temperature setting range of 30 to 105℃. It features semiconductor Peltier technology, ensuring reliable amplification and long-term operational stability. Equipped with self-adjusting heated lid, it adapts to various tube heights and prevents sample evaporation. Incorporates automatic fault detection and alarm function for safe operation and enhanced laboratory safety. Thermal Cycler is ideal for gradient PCR, DNA amplification, genetic testing, and protocol optimization.

Manufactured under strict quality control processes, complying with international laboratory equipment standards. Provides 1-year warranty for main components and lifetime technical support.

Applications

Gradient Thermal Cycler is applied for gradient PCR, DNA amplification, protocol optimization, and genetic testing in molecular biology, food analysis, medical diagnostics, and clinical research laboratories.

Specifications

Capacity 96×0.2ml (0.2ml PCR tubes, 8-tube strips)
Gradient Temperature Range 30 to 105℃
Gradient Setting Range 0.1 to 30℃
Gradient Temperature Uniformity Less than or equal to ±0.2℃
Gradient Temperature Accuracy Less than or equal to ±0.2℃
Temperature Setting Range 4 to 105℃
Heated Lid Temperature Range 30 to 115℃ (adjustable)
Temperature Uniformity Less than or equal to ±0.2℃
Temperature Accuracy Less than or equal to ±0.2℃
Maximum Heating Rate 5℃/sec
Maximum Cooling Rate 4℃/sec
Temperature Display Resolution 0.1℃
Temperature Control Method Block/Tub
Display 7-inch color touch screen, 800×480 Pixel
Communication Interface USB
Tm Calculation Automated
Program Memory 10000+
Time Increment/Decrement -999 to 999s
Temperature Increment/Decrement -10 to 10℃
Temperature Change Rate 0.1 to 4℃/s
Single Step Time Range 0 to 9h59min59s/∞
Maximum Cycle Numbers 100
Maximum Program Steps 30
Program Pause Function Yes
16℃ Insulation Forever
On-Screen Report Yes
Fuse 250V 10A Ф5×20
Power Consumption 800W
Power Supply AC100 to 240V, 50 to 60Hz
Dimensions 420×260×245 mm
Weight 11 Kg

Features

Rapid heating and cooling

Easy programming

Procedure templates

Quick editing

On-screen reporting

Run completion display

FAQs

1. What is a Gradient Thermal Cycler?

A Gradient Thermal Cycler is a PCR machine designed for simultaneous testing of different annealing temperatures across a temperature gradient.

2. How does Gradient Thermal Cycler work?

It works by creating a temperature gradient across the block, enabling multiple PCR reactions to be run at slightly different temperatures in the same cycle.

3. Can Gradient Thermal Cycler be used for multiplex PCR?

It is ideal for multiplex PCR, as it helps determine a temperature that works efficiently for multiple primer sets in the same reaction.

4. How does Gradient Thermal Cycler improve PCR efficiency in research labs?

It improves PCR efficiency in research labs by reducing trial-and-error cycles and enabling faster optimization of reaction conditions.

5. Is Gradient Thermal Cycler useful in genotyping applications?

It is widely used in genotyping to optimize conditions for amplifying specific alleles or polymorphisms accurately.

6. How does Gradient Thermal Cycler support environmental DNA (eDNA) analysis?

It supports eDNA analysis by enabling rapid optimization of PCR conditions for complex, low-abundance DNA targets in environmental samples.

7. Is Gradient Thermal Cycler beneficial for forensic DNA analysis?

It is highly beneficial for forensic DNA analysis, where precise optimization is required to amplify minute or degraded DNA samples reliably.

8. How does Gradient Thermal Cycler support microbiome or metagenomics studies?

It supports microbiome and metagenomics studies by enabling precise amplification of complex microbial DNA mixtures with optimized conditions.

9. Can Gradient Thermal Cycler be used for pathogen detection in food safety testing?

It is commonly used in food safety testing to optimize PCR conditions for detecting bacterial, viral, or fungal pathogens in food samples.

10. How does Gradient Thermal Cycler support molecular diagnostics in clinical labs?

It supports molecular diagnostics by allowing rapid and accurate PCR optimization for disease-specific genetic markers.

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