Gradient PCR T-GP-501

Introduction

Gradient PCR is small in size with compact structure. It is a gene amplification instrument with gradient function derived from ordinary PCR instrument. Equipped with 5 inch high definition color touch screen, intuitive and easy to operate. High accurate temperature with good uniformity, quick heating up and cooling down. Data is quickly transferred to USB flash memory and programs are easily backed up to improve data storage capabilities. Features advanced semiconductor refrigeration technology.

Manufactured under strict quality control processes, complying with international laboratory equipment standards. Provides 1-year warranty for main components and lifetime technical support.

Applications

Widely applied in the field of molecular biology, medicine, food industry, genetic testing and other fields

Selection Guide

Gradient PCR is a versatile and efficient thermal cycler designed for applications such as gene cloning, mutagenesis, molecular diagnostics, and PCR protocol optimization. Its gradient function allows simultaneous testing of multiple annealing temperatures, making it perfect for researchers developing or refining assays. With a 96 × 0.2 ml sample capacity, accurate temperature control, and fast heating/cooling rates, it ensures accurate and reproducible results. The user-friendly interface simplifies programming and operation, even for complex protocols. Certified with ISO and CE markings, this model meets international standards for quality and safety, making it a reliable choice for research and diagnostic laboratories seeking consistent and high-throughput PCR performance.

Specifications

Temperature control mode	Block or tube
Module Temperature Range	4 to 99.9°C
Timing Range	1s to 99 min 59sec/infinite length
Module temperature accuracy	±0.2°C
Module Temperature Uniformity	±0.25°C
Heating Rate	3°C
Cooling Rate	2.5°C
Maximum Heating Speed	4.5°C/S
Maximum Cooling Speed	4.0°C
Input Power	AC 100 to 240V, 50/60 Hz
Gradient Temperature Uniformity	±0.3°C
Temperature Module Displays the Accuracy	0.1°C
Thermal Cap Temperature Range	Room Temperature 30°C to 105°C
Smart Hot Cap Function	Sample Temperature is below 30°C or at the end of the program, the hot cap closes automatically
Number of programs stored	>100 pcs
Maximum Number of cycles	99
Maximum Steps	30
Program Pause Function	Yes
16°C insulation Function	Infinitely Long
Real Time Running Status Display	Graphic Display Mode
Communication Interface	USB 2.0
Dimensions (W×D×H)	200×300×170 mm
Net Weight	4.5 Kg
Sample Size	96×0.2 ml (Skirt, Skirtless) 12×8 intertube, 8×12 coupling, single tube( height 20 to 23 mm)

Features

Excellent Amplification due to high quality semiconductor
Industrial grade operation system and can work 7×24 hours
30°C gradient range, ideal for protocol optimization
PID temperature control technology
High Temperature control accuracy and module temperature uniformity
Extremely quiet

FAQs

1. What is a Gradient PCR

A Gradient PCR is a laboratory instrument designed for polymerase chain reaction (PCR) with a gradient function that allows temperature optimization across multiple samples.

2. Can Gradient PCR be used for genotyping applications?

Gradient PCR is ideal for genotyping by enabling precise temperature optimization.

3. How can Gradient PCR be used in mutation detection?

Gradient PCR helps detect mutations by enabling temperature gradient PCR, which can differentiate between wild-type and mutant sequences through optimized annealing conditions.

4. Can Gradient PCR be used for cloning applications?

Gradient PCR is ideal for cloning applications as it allows optimization of PCR conditions to ensure high-fidelity amplification of target DNA sequences.

5. How does Gradient PCR assist in forensic DNA analysis?

Gradient PCR aids forensic DNA analysis by enabling precise temperature control to amplify degraded or low-concentration DNA samples efficiently.

6. Can Gradient PCR be used for pathogen identification?

Gradient PCR enables temperature optimization for accurate pathogen identification through PCR amplification.

7. How is Gradient PCR useful in environmental DNA studies?

Gradient PCR supports environmental DNA studies by optimizing amplification from diverse and low-concentration DNA samples.

8. How does Gradient PCR assist in cancer mutation screening?

Gradient PCR aids cancer mutation screening by enabling temperature optimization to detect mutations with high specificity.

9. Can Gradient PCR be used for metagenomic studies?

Gradient PCR facilitates metagenomic studies by allowing gradient optimization for complex microbial community DNA amplification.

10. Can Gradient PCR support viral genome detection in clinical diagnostics?

Gradient PCR supports viral genome detection in diagnostics by optimizing primer annealing conditions for high sensitivity.

11. What is a Gradient PCR?

A Gradient PCR is an apparatus designed for identifying the optimal annealing temperature for a specific PCR reaction.

12. Is Gradient PCR faster than running multiple individual PCR reactions?

It can test multiple temperatures in one experiment thereby saving time and providing quick results.

13. Can Gradient PCR be used for different sets of primers?

It is used for testing different sets of primers to find the best annealing temperature for each set.

14. Is Gradient PCR suitable for all types of DNA?

It can be used with various types of DNA templates such as genomic DNA, plasmid DNA, and cDNA.

15. Can Gradient PCR help with low-abundance templates?

It can identify the best conditions for amplification of low-abundance templates thereby improving the chances of getting good results.

16. Can Gradient PCR be used for amplifying large DNA fragments?

It can be used to amplify large DNA fragments.

17. Can Gradient PCR be used for DNA amplification from environmental samples?

It can be used for amplifying DNA from environmental samples such as soil, water or biological samples.

18. Can Gradient PCR be used for diagnostic PCR assays?

It can be used for diagnostic PCR assays.