Gradient Thermal Cycler T-GT-L2201

Introduction

Gradient thermal cycler enabled with Peltier heating units which can process more than 99 cycles. It can accommodate 96×0.2 ml sample in wells. User-friendly programming is achieved via 5.7inch LCD with graphical display and windows operations system. This device can be applied across a temperature range of 0 to 99.9 °C with maximum ramping rate is 4.5 °C/s.

Manufactured under strict quality control processes, complying with international laboratory equipment standards. Provides 1-year warranty for main components and lifetime technical support.

Applications

Used in Gene expression analysis, Pathogen detection, DNA methylation analysis, Genotyping and gene scanning, miRNA research etc.

Widely applied in academic research laboratories, quality control departments of manufacturing enterprises, and testing institutions. Suitable for sample preparation, analysis, and storage in fields such as life sciences, materials science, environmental monitoring, and food safety.

Specifications

Capacity	96×0.2 ml
Temperature Range	0 to 99.9 °C
Maximum. Heating Rate	4.5 °C/s
Maximum. Cooling Rate	4 °C/s
Uniformity	≤ ± 0.3 °C
Accuracy	≤ ± 0.2 °C
Display Resolution	0.1 °C
Temperature Control	BlockTube
Ramping Rate Adjustable	0.1 to 4.5 °C
Gradient Uniformity	≤ ± 0.3 °C
Gradient Accuracy	≤ ± 0.2 °C
Gradient Temperature. Range	30 to 99.9 °C
Gradient Spread	1 to 30 °C
Hot Lid Temperature	30 to 110 °C
Hot Lid Height Adjustable	Step less Adjustable
Number Of Programs	200
Maximum .No.Of Segment	9
Maximum. No. Of Step	9
Maximum. No. Of Cycle	99
Increment/Decrement Time	1 Second to 9 Min 59 Second
Increment/Decrement Temperature	0.1 to 9.9 °C
Pause Function	Yes
Auto Data Protection	Yes
Graphical Display	Yes
Holding	4 °C
LCD	5.7-inch, 320×240 pels
Dimensions(L×W×H)	392×262×252 mm
Weight	10 kg
Power Consumption	600 W
Power Supply	85 to 264 V AC , 50 to 60 Hz

Features

Uniform heating of the samples is achieved with Peltier heating units with more than 99 cycles
Maximum. heating Rate is 4.5 °C/s with temperature range of 0 to 99.9 °C
It has the capability to accommodate 96×0.2 ml, 96×0.2 ml+77×0.5 ml with moveable hot lid which can fit different sizes of tubes
All the operations are controlled by a computer software system which instrument PCR protocols
It has power of operation recovery option which helps in continuing the same command after a recovering from power cut
To satisfy the customers need it is installed with special feature like the user can add commands while the process is going on to encounter different variations

FAQs

1. What is a Gradient Thermal Cycler

Gradient Thermal Cycler is a PCR machine that enables precise DNA amplification with programmable temperature gradients for optimizing annealing conditions.

2. How does a Gradient Thermal Cycler work?

Gradient Thermal Cycler operates on the principle of thermal cycling to amplify DNA through denaturation, annealing, and extension.

3. Can Gradient Thermal Cycler perform DNA methylation studies?

Gradient Thermal Cycler enables optimization for bisulfite-treated DNA amplification.

4. Can Gradient Thermal Cycler be used for genotyping experiments?

Gradient Thermal Cycler accommodates high-throughput genotyping with consistent performance.

5. Can Gradient Thermal Cycler help analyze microbial spoilage in packaged foods?

Gradient Thermal Cycler detects spoilage organisms by amplifying species-specific DNA markers.

6. Can Gradient Thermal Cycler be used to assess gene expression biomarkers in cancer diagnostics?

Gradient Thermal Cycler amplifies tumor-specific gene markers reliably for diagnostic panels.

7. Does Gradient Thermal Cycler support gene expression studies in enzyme production optimization?

Gradient Thermal Cycler assists in profiling gene expression to fine-tune industrial enzyme yields.

8. Can Gradient Thermal Cycler assist in fermentation process control through gene expression profiling?

Gradient Thermal Cycler enables batch-wise monitoring of microbial gene activity during industrial fermentation.

9. Is Gradient Thermal Cycler useful for veterinary pharmaceutical manufacturing for gene expression checks in vaccine strains?

Gradient Thermal Cycler accurately monitors antigen-related gene expression in vaccine development workflows.

10. What kind of maintenance does Advanced Gradient Thermal Cycler require?

Routine maintenance includes regular cleaning of the block and lid, ventilation checks, firmware updates, and annual calibration.

11. What is a Gradient Thermal Cycler?

A Gradient Thermal Cycler is a PCR machine designed for simultaneous testing of different annealing temperatures across a temperature gradient.

12. How does Gradient Thermal Cycler work?

It works by creating a temperature gradient across the block, enabling multiple PCR reactions to be run at slightly different temperatures in the same cycle.

13. Can Gradient Thermal Cycler be used for multiplex PCR?

It is ideal for multiplex PCR, as it helps determine a temperature that works efficiently for multiple primer sets in the same reaction.

14. How does Gradient Thermal Cycler improve PCR efficiency in research labs?

It improves PCR efficiency in research labs by reducing trial-and-error cycles and enabling faster optimization of reaction conditions.

15. Is Gradient Thermal Cycler useful in genotyping applications?

It is widely used in genotyping to optimize conditions for amplifying specific alleles or polymorphisms accurately.

16. How does Gradient Thermal Cycler support environmental DNA (eDNA) analysis?

It supports eDNA analysis by enabling rapid optimization of PCR conditions for complex, low-abundance DNA targets in environmental samples.

17. Is Gradient Thermal Cycler beneficial for forensic DNA analysis?

It is highly beneficial for forensic DNA analysis, where precise optimization is required to amplify minute or degraded DNA samples reliably.

18. How does Gradient Thermal Cycler support microbiome or metagenomics studies?

It supports microbiome and metagenomics studies by enabling precise amplification of complex microbial DNA mixtures with optimized conditions.

19. Can Gradient Thermal Cycler be used for pathogen detection in food safety testing?

It is commonly used in food safety testing to optimize PCR conditions for detecting bacterial, viral, or fungal pathogens in food samples.

20. How does Gradient Thermal Cycler support molecular diagnostics in clinical labs?

It supports molecular diagnostics by allowing rapid and accurate PCR optimization for disease-specific genetic markers.