Introduction
Advanced Thermal Cycler (Gradient) B200 features wide temperature range from 0°C to 100°C. It features dual block configuration with 48 wells for 0.2 mL tubes and 48 wells for 0.5 mL tubes. Equipped with USB or LAN updates for new features and software enhancements. Incorporated touchscreen interface simplifies programming and operation. Advanced Thermal Cycler (Gradient) supports PCR, DNA analysis, gene expression, pathogen detection, and environmental testing.
Manufactured under strict quality control processes, complying with international laboratory equipment standards. Provides 1-year warranty for main components and lifetime technical support.
Applications
Advanced Thermal Cycler (Gradient) is widely used for PCR, DNA quantification, and gene expression studies. It also supports applications like pathogen detection and environmental testing across various fields.
Specifications
| Block Capacity | 48 wells × 0.2 mL + 48 wells × 0.5 mL |
| Block Temperature Range | 0 ℃ to 100 ℃ |
| Temperature Accuracy | ≤ ± 0.2℃ (35 ℃ ~ 100 ℃) |
| Temperature Uniformity | ≤ ± 0.2℃ |
| Ramping Rate | Adjustable |
| Heating Rate | 4℃/s |
| Cooling Rate | 3.5 ℃/s |
| Display Resolution | 0.1℃ |
| Gradient Temperature Range | 30 ℃ to 99.9 ℃ |
| Gradient Temperature Accuracy | ≤ ± 0.3 ℃ (35 ℃ - 99.9 ℃) |
| Gradient Temperature Uniformity | ≤ ± 0.3 ℃ |
| Gradient Capacity | 12 Columns (Vertical) |
| Temperature Differential Range | 1℃ - 30 ℃ |
| Hot-lid Temperature Range | 30 ℃ to 105 ℃ |
| Max No. of Steps | 30 |
| Max No. of Program | 120 |
| Max No. of Cycles | 100 |
| Increment / Decrement Time | 1 to 120 sec (Long PCR) |
| Increment / Decrement Temperature | 0.1 ℃ to 10.0 ℃ (Touchdown PCR) |
| Display | 5.7 |
| Data Output Port | USB 2.0 / RS232 Port |
| Power Consumption | 600 W |
| Power Supply | AC 175 / 275 V; 50 / 60 Hz |
| Dimension | 335 × 240 × 270 mm |
| Net Weight | 9.8 Kg |
Features
Fast thermal ramping
Android OS with programs
Compact design with air vents
Auto self-closing hot lid
Corrosion-resistant aluminum build
Adjustable settings mid-run
Power-off recovery system
FAQs
1. What is an Advanced Thermal Cycler (Gradient)
An Advanced Thermal Cycler (Gradient) is a high-precision PCR machine with gradient temperature capability, designed for efficient nucleic acid amplification and molecular biology applications.
2. How does Advanced Thermal Cycler (Gradient) work?
An Advanced Thermal Cycler (Gradient) operates on the principle of thermal cycling, involving repeated heating and cooling to denature DNA, anneal primers, and extend new strands during the PCR process.
3. Can Advanced Thermal Cycler (Gradient) be used for genetic testing in human or animal tissues?
The Advanced Thermal Cycler (Gradient) amplifies DNA from tissue samples for genetic screening, disease diagnostics, or trait analysis.
4. Is Advanced Thermal Cycler (Gradient) suitable for plant DNA analysis?
Advanced Thermal Cycler (Gradient) efficiently amplifies plant genomic DNA for GMO detection, phylogenetic analysis and breeding research.
5. Can Advanced Thermal Cycler (Gradient) be used for blood-based disease detection?
Advanced Thermal Cycler (Gradient) is used to extract DNA or RNA from blood, to detect pathogens or genetic markers linked to diseases.
6. Is Advanced Thermal Cycler (Gradient) useful for detecting viral infections?
Advanced Thermal Cycler (Gradient) amplifies viral RNA or DNA to detect viruses such as influenza or COVID-19.
7. Can Advanced Thermal Cycler (Gradient) be used to analyze microbial DNA from soil or water samples?
Advanced Thermal Cycler (Gradient) is suitable for eDNA studies to detect microbial or fungal species in soil or water samples.
8. Can Advanced Thermal Cycler (Gradient) be used for detecting GMOs in food products?
Advanced Thermal Cycler (Gradient) amplifies specific DNA sequences to identify genetically modified organisms in food and agricultural products.
9. Is Advanced Thermal Cycler (Gradient) applicable for forensic DNA analysis?
Advanced Thermal Cycler (Gradient) amplifies trace DNA samples for use in forensic identification.
10. What kind of maintenance does Advanced Thermal Cycler (Gradient) require?
Regular maintenance includes cleaning the block, lid, and vents, and performing regular temperature calibration for accurate results.
11. Can Advanced Thermal Cycler be used for DNA damage and repair studies?
It is used in DNA damage and repair studies to amplify damaged or repaired sequences and analyze repair pathway activity.
12. What is an Advanced Thermal Cycler?
An Advanced Thermal Cycler is a laboratory apparatus designed to amplify DNA segments by precisely controlling the temperature cycles required for polymerase chain reaction (PCR).
13. How does Advanced Thermal Cycler work?
It works by rapidly heating and cooling samples through programmed temperature steps, allowing DNA denaturation, annealing, and extension to occur during PCR.
14. How does Advanced Thermal Cycler support forensic DNA analysis?
It supports forensic DNA analysis by amplifying trace amounts of DNA from crime scene evidence for identification purposes.
15. How is Advanced Thermal Cycler applied in clinical diagnostics?
It is applied in clinical diagnostics to detect genetic disorders, infectious diseases, and cancer biomarkers through PCR-based tests.
16. Can Advanced Thermal Cycler be used in food safety testing?
It is used in food safety testing to detect bacterial contamination, allergens, and genetically modified organisms (GMOs).
17. Can Advanced Thermal Cycler be used in pharmacogenomics research in the lab?
It is used in pharmacogenomics to analyze genetic variations that affect drug metabolism and response, supporting personalized medicine.
18. How does Advanced Thermal Cycler support high-throughput screening in the lab?
It supports high-throughput screening by enabling simultaneous PCR amplification of multiple targets across 96- or 384-well plates.
19. Can Advanced Thermal Cycler be used for detecting antibiotic resistance genes in the lab?
It is used to amplify and detect antibiotic resistance genes in bacterial isolates through targeted PCR assays.
20. What role does Advanced Thermal Cycler play in metagenomic sample preparation?
It is used in metagenomics to amplify target microbial DNA from environmental or clinical samples prior to sequencing or analysis.
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